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Hepatic endoplasmic reticulum calcium fluxes: effect of free fatty acids and K(ATP) channel involvement
As a common sequel to obesity, plasma and intracellular free fatty acid (FFA) concentrations are elevated and, as a consequence, manifold disturbances in metabolism may ensue. Biochemical processes in the cytosol and organelles, such as mitochondria and endoplasmic reticulum (ER), can be disturbed....
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Portland Press Ltd.
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7851409/ https://www.ncbi.nlm.nih.gov/pubmed/33442738 http://dx.doi.org/10.1042/BSR20202940 |
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author | Al-Rawi, Rawan Wang, Xudong McCormick, Kenneth |
author_facet | Al-Rawi, Rawan Wang, Xudong McCormick, Kenneth |
author_sort | Al-Rawi, Rawan |
collection | PubMed |
description | As a common sequel to obesity, plasma and intracellular free fatty acid (FFA) concentrations are elevated and, as a consequence, manifold disturbances in metabolism may ensue. Biochemical processes in the cytosol and organelles, such as mitochondria and endoplasmic reticulum (ER), can be disturbed. In the ER, the maintenance of a high calcium gradient is indispensable for viability. In sarcoplasmic reticulum, selective FFA can induce ER stress by disrupting luminal calcium homeostasis; however, there are limited studies in hepatic microsomes. Our studies found that FFA has a noxious effect on rat hepatic microsomal calcium flux, and the extent of which depended on the number of double bonds and charge. Furthermore, insofar as the FFA had no effect on microsomal calcium efflux, their inhibitory action primarily involves calcium influx. Finally, other cationic channels have been found in hepatic ER, and evidence is presented of their interaction with the Ca(2+) ATPase pump. |
format | Online Article Text |
id | pubmed-7851409 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Portland Press Ltd. |
record_format | MEDLINE/PubMed |
spelling | pubmed-78514092021-02-08 Hepatic endoplasmic reticulum calcium fluxes: effect of free fatty acids and K(ATP) channel involvement Al-Rawi, Rawan Wang, Xudong McCormick, Kenneth Biosci Rep Cell Membranes, Excitation & Transport As a common sequel to obesity, plasma and intracellular free fatty acid (FFA) concentrations are elevated and, as a consequence, manifold disturbances in metabolism may ensue. Biochemical processes in the cytosol and organelles, such as mitochondria and endoplasmic reticulum (ER), can be disturbed. In the ER, the maintenance of a high calcium gradient is indispensable for viability. In sarcoplasmic reticulum, selective FFA can induce ER stress by disrupting luminal calcium homeostasis; however, there are limited studies in hepatic microsomes. Our studies found that FFA has a noxious effect on rat hepatic microsomal calcium flux, and the extent of which depended on the number of double bonds and charge. Furthermore, insofar as the FFA had no effect on microsomal calcium efflux, their inhibitory action primarily involves calcium influx. Finally, other cationic channels have been found in hepatic ER, and evidence is presented of their interaction with the Ca(2+) ATPase pump. Portland Press Ltd. 2021-02-01 /pmc/articles/PMC7851409/ /pubmed/33442738 http://dx.doi.org/10.1042/BSR20202940 Text en © 2021 The Author(s). https://creativecommons.org/licenses/by/4.0/ This is an open access article published by Portland Press Limited on behalf of the Biochemical Society and distributed under the Creative Commons Attribution License 4.0 (CC BY) (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Cell Membranes, Excitation & Transport Al-Rawi, Rawan Wang, Xudong McCormick, Kenneth Hepatic endoplasmic reticulum calcium fluxes: effect of free fatty acids and K(ATP) channel involvement |
title | Hepatic endoplasmic reticulum calcium fluxes: effect of free fatty acids and K(ATP) channel involvement |
title_full | Hepatic endoplasmic reticulum calcium fluxes: effect of free fatty acids and K(ATP) channel involvement |
title_fullStr | Hepatic endoplasmic reticulum calcium fluxes: effect of free fatty acids and K(ATP) channel involvement |
title_full_unstemmed | Hepatic endoplasmic reticulum calcium fluxes: effect of free fatty acids and K(ATP) channel involvement |
title_short | Hepatic endoplasmic reticulum calcium fluxes: effect of free fatty acids and K(ATP) channel involvement |
title_sort | hepatic endoplasmic reticulum calcium fluxes: effect of free fatty acids and k(atp) channel involvement |
topic | Cell Membranes, Excitation & Transport |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7851409/ https://www.ncbi.nlm.nih.gov/pubmed/33442738 http://dx.doi.org/10.1042/BSR20202940 |
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