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Reversible inhibition of sperm under guidance as an intratubular and reversible contraception in female rats: An experimental study
BACKGROUND: Reversible inhibition of sperm under guidance (“RISUGⓇ”) is a promising intravasal male contraceptive. OBJECTIVE: An exploratory study was conducted with a concept of non-invasive, transcervical, single-intervention and reversible contraception using RISUGⓇ in females. MATERIALS AND METH...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Knowledge E
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7851472/ https://www.ncbi.nlm.nih.gov/pubmed/33554002 http://dx.doi.org/10.18502/ijrm.v19i1.8179 |
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author | Salam Ansari, Abdul Sevliya, Kiran Badar, Ayesha Lohiya, Nirmal Kumar |
author_facet | Salam Ansari, Abdul Sevliya, Kiran Badar, Ayesha Lohiya, Nirmal Kumar |
author_sort | Salam Ansari, Abdul |
collection | PubMed |
description | BACKGROUND: Reversible inhibition of sperm under guidance (“RISUGⓇ”) is a promising intravasal male contraceptive. OBJECTIVE: An exploratory study was conducted with a concept of non-invasive, transcervical, single-intervention and reversible contraception using RISUGⓇ in females. MATERIALS AND METHODS: In this experimental study, 60 adult Wistar albino female rats weighing 150-155 g, 3-4 months old were divided into four groups: group I: sham-operated control; group II: tubal occlusion with RISUG for 90 days; group III: tubal occlusion with RISUGⓇ for 90 days and reversal with dimethyl sulphoxide and group IV: tubal occlusion with RISUGⓇ for 90 days and reversal with 5% NaHCO. Animals were subjected to bilateral fallopian tube occlusion with RISUGⓇ and reversal with DMSO and NaHCO [Formula: see text]. The estrous cycle, fertility and histology of fallopian tube were evaluated. RESULTS: Group I showed 100% fertility during all mating schedules. Animals of experimental groups indicated positive mating, but 0% fertility was evident following 30, 60, and 90 days of tubal occlusion. However, after reversal, fertility steadily increased to normalcy in groups III (50% at 45 days, 80% at 105 days, 100% at 150 and 195 days) and IV (70% at 45 and 105 days, 100% at 150 and 195 days) animals. Group II illustrated disorganized inner cell linings and eosinated RISUGⓇimplant-filled lumen. Reversal groups (III and IV) revealed complete restoration of cellular histo-architecture. Regular estrous cycle was noticed in all experimental groups. CONCLUSION: RISUGⓇ is suitable for single intervention, intratubular, reversible contraception in female rats. |
format | Online Article Text |
id | pubmed-7851472 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Knowledge E |
record_format | MEDLINE/PubMed |
spelling | pubmed-78514722021-02-05 Reversible inhibition of sperm under guidance as an intratubular and reversible contraception in female rats: An experimental study Salam Ansari, Abdul Sevliya, Kiran Badar, Ayesha Lohiya, Nirmal Kumar Int J Reprod Biomed Research Article BACKGROUND: Reversible inhibition of sperm under guidance (“RISUGⓇ”) is a promising intravasal male contraceptive. OBJECTIVE: An exploratory study was conducted with a concept of non-invasive, transcervical, single-intervention and reversible contraception using RISUGⓇ in females. MATERIALS AND METHODS: In this experimental study, 60 adult Wistar albino female rats weighing 150-155 g, 3-4 months old were divided into four groups: group I: sham-operated control; group II: tubal occlusion with RISUG for 90 days; group III: tubal occlusion with RISUGⓇ for 90 days and reversal with dimethyl sulphoxide and group IV: tubal occlusion with RISUGⓇ for 90 days and reversal with 5% NaHCO. Animals were subjected to bilateral fallopian tube occlusion with RISUGⓇ and reversal with DMSO and NaHCO [Formula: see text]. The estrous cycle, fertility and histology of fallopian tube were evaluated. RESULTS: Group I showed 100% fertility during all mating schedules. Animals of experimental groups indicated positive mating, but 0% fertility was evident following 30, 60, and 90 days of tubal occlusion. However, after reversal, fertility steadily increased to normalcy in groups III (50% at 45 days, 80% at 105 days, 100% at 150 and 195 days) and IV (70% at 45 and 105 days, 100% at 150 and 195 days) animals. Group II illustrated disorganized inner cell linings and eosinated RISUGⓇimplant-filled lumen. Reversal groups (III and IV) revealed complete restoration of cellular histo-architecture. Regular estrous cycle was noticed in all experimental groups. CONCLUSION: RISUGⓇ is suitable for single intervention, intratubular, reversible contraception in female rats. Knowledge E 2021-01-25 /pmc/articles/PMC7851472/ /pubmed/33554002 http://dx.doi.org/10.18502/ijrm.v19i1.8179 Text en Copyright © 2021 Ansari et al. https://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. which permits unrestricted use and redistribution provided that the original author and source are credited. |
spellingShingle | Research Article Salam Ansari, Abdul Sevliya, Kiran Badar, Ayesha Lohiya, Nirmal Kumar Reversible inhibition of sperm under guidance as an intratubular and reversible contraception in female rats: An experimental study |
title | Reversible inhibition of sperm under guidance as an intratubular and reversible contraception in female rats: An experimental study |
title_full | Reversible inhibition of sperm under guidance as an intratubular and reversible contraception in female rats: An experimental study |
title_fullStr | Reversible inhibition of sperm under guidance as an intratubular and reversible contraception in female rats: An experimental study |
title_full_unstemmed | Reversible inhibition of sperm under guidance as an intratubular and reversible contraception in female rats: An experimental study |
title_short | Reversible inhibition of sperm under guidance as an intratubular and reversible contraception in female rats: An experimental study |
title_sort | reversible inhibition of sperm under guidance as an intratubular and reversible contraception in female rats: an experimental study |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7851472/ https://www.ncbi.nlm.nih.gov/pubmed/33554002 http://dx.doi.org/10.18502/ijrm.v19i1.8179 |
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