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Reversible inhibition of sperm under guidance as an intratubular and reversible contraception in female rats: An experimental study

BACKGROUND: Reversible inhibition of sperm under guidance (“RISUGⓇ”) is a promising intravasal male contraceptive. OBJECTIVE: An exploratory study was conducted with a concept of non-invasive, transcervical, single-intervention and reversible contraception using RISUGⓇ in females. MATERIALS AND METH...

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Autores principales: Salam Ansari, Abdul, Sevliya, Kiran, Badar, Ayesha, Lohiya, Nirmal Kumar
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Knowledge E 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7851472/
https://www.ncbi.nlm.nih.gov/pubmed/33554002
http://dx.doi.org/10.18502/ijrm.v19i1.8179
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author Salam Ansari, Abdul
Sevliya, Kiran
Badar, Ayesha
Lohiya, Nirmal Kumar
author_facet Salam Ansari, Abdul
Sevliya, Kiran
Badar, Ayesha
Lohiya, Nirmal Kumar
author_sort Salam Ansari, Abdul
collection PubMed
description BACKGROUND: Reversible inhibition of sperm under guidance (“RISUGⓇ”) is a promising intravasal male contraceptive. OBJECTIVE: An exploratory study was conducted with a concept of non-invasive, transcervical, single-intervention and reversible contraception using RISUGⓇ in females. MATERIALS AND METHODS: In this experimental study, 60 adult Wistar albino female rats weighing 150-155 g, 3-4 months old were divided into four groups: group I: sham-operated control; group II: tubal occlusion with RISUG for 90 days; group III: tubal occlusion with RISUGⓇ for 90 days and reversal with dimethyl sulphoxide and group IV: tubal occlusion with RISUGⓇ for 90 days and reversal with 5% NaHCO. Animals were subjected to bilateral fallopian tube occlusion with RISUGⓇ and reversal with DMSO and NaHCO [Formula: see text]. The estrous cycle, fertility and histology of fallopian tube were evaluated. RESULTS: Group I showed 100% fertility during all mating schedules. Animals of experimental groups indicated positive mating, but 0% fertility was evident following 30, 60, and 90 days of tubal occlusion. However, after reversal, fertility steadily increased to normalcy in groups III (50% at 45 days, 80% at 105 days, 100% at 150 and 195 days) and IV (70% at 45 and 105 days, 100% at 150 and 195 days) animals. Group II illustrated disorganized inner cell linings and eosinated RISUGⓇimplant-filled lumen. Reversal groups (III and IV) revealed complete restoration of cellular histo-architecture. Regular estrous cycle was noticed in all experimental groups. CONCLUSION: RISUGⓇ is suitable for single intervention, intratubular, reversible contraception in female rats.
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spelling pubmed-78514722021-02-05 Reversible inhibition of sperm under guidance as an intratubular and reversible contraception in female rats: An experimental study Salam Ansari, Abdul Sevliya, Kiran Badar, Ayesha Lohiya, Nirmal Kumar Int J Reprod Biomed Research Article BACKGROUND: Reversible inhibition of sperm under guidance (“RISUGⓇ”) is a promising intravasal male contraceptive. OBJECTIVE: An exploratory study was conducted with a concept of non-invasive, transcervical, single-intervention and reversible contraception using RISUGⓇ in females. MATERIALS AND METHODS: In this experimental study, 60 adult Wistar albino female rats weighing 150-155 g, 3-4 months old were divided into four groups: group I: sham-operated control; group II: tubal occlusion with RISUG for 90 days; group III: tubal occlusion with RISUGⓇ for 90 days and reversal with dimethyl sulphoxide and group IV: tubal occlusion with RISUGⓇ for 90 days and reversal with 5% NaHCO. Animals were subjected to bilateral fallopian tube occlusion with RISUGⓇ and reversal with DMSO and NaHCO [Formula: see text]. The estrous cycle, fertility and histology of fallopian tube were evaluated. RESULTS: Group I showed 100% fertility during all mating schedules. Animals of experimental groups indicated positive mating, but 0% fertility was evident following 30, 60, and 90 days of tubal occlusion. However, after reversal, fertility steadily increased to normalcy in groups III (50% at 45 days, 80% at 105 days, 100% at 150 and 195 days) and IV (70% at 45 and 105 days, 100% at 150 and 195 days) animals. Group II illustrated disorganized inner cell linings and eosinated RISUGⓇimplant-filled lumen. Reversal groups (III and IV) revealed complete restoration of cellular histo-architecture. Regular estrous cycle was noticed in all experimental groups. CONCLUSION: RISUGⓇ is suitable for single intervention, intratubular, reversible contraception in female rats. Knowledge E 2021-01-25 /pmc/articles/PMC7851472/ /pubmed/33554002 http://dx.doi.org/10.18502/ijrm.v19i1.8179 Text en Copyright © 2021 Ansari et al. https://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. which permits unrestricted use and redistribution provided that the original author and source are credited.
spellingShingle Research Article
Salam Ansari, Abdul
Sevliya, Kiran
Badar, Ayesha
Lohiya, Nirmal Kumar
Reversible inhibition of sperm under guidance as an intratubular and reversible contraception in female rats: An experimental study
title Reversible inhibition of sperm under guidance as an intratubular and reversible contraception in female rats: An experimental study
title_full Reversible inhibition of sperm under guidance as an intratubular and reversible contraception in female rats: An experimental study
title_fullStr Reversible inhibition of sperm under guidance as an intratubular and reversible contraception in female rats: An experimental study
title_full_unstemmed Reversible inhibition of sperm under guidance as an intratubular and reversible contraception in female rats: An experimental study
title_short Reversible inhibition of sperm under guidance as an intratubular and reversible contraception in female rats: An experimental study
title_sort reversible inhibition of sperm under guidance as an intratubular and reversible contraception in female rats: an experimental study
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7851472/
https://www.ncbi.nlm.nih.gov/pubmed/33554002
http://dx.doi.org/10.18502/ijrm.v19i1.8179
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