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Creation of Universal Primers Targeting Nonconserved, Horizontally Mobile Genes: Lessons and Considerations
Effective and accurate primer design is an increasingly important skill, as the use of PCR-based diagnostics in clinical and environmental settings is on the rise. While universal primer sets have been successfully designed for highly conserved core genes, such as 16S rRNA, and characteristic genes,...
| Autores principales: | , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
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American Society for Microbiology
2021
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| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7851684/ https://www.ncbi.nlm.nih.gov/pubmed/33277268 http://dx.doi.org/10.1128/AEM.02181-20 |