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Creation of Universal Primers Targeting Nonconserved, Horizontally Mobile Genes: Lessons and Considerations

Effective and accurate primer design is an increasingly important skill, as the use of PCR-based diagnostics in clinical and environmental settings is on the rise. While universal primer sets have been successfully designed for highly conserved core genes, such as 16S rRNA, and characteristic genes,...

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Detalles Bibliográficos
Autores principales: Brown, Damon C., Turner, Raymond J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Society for Microbiology 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7851684/
https://www.ncbi.nlm.nih.gov/pubmed/33277268
http://dx.doi.org/10.1128/AEM.02181-20

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