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Simiaosan alleviates the symptoms of gouty arthritis via the NALP3/IL-1β pathway
Previous studies have suggested that the herbal medicine simiaosan has beneficial effects on gouty arthritis (GA), for which conventional Western medicines are insufficient (particularly in cases of multiple episodes). The objective of the present study was to investigate the mechanism by which simi...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
D.A. Spandidos
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7851828/ https://www.ncbi.nlm.nih.gov/pubmed/33655332 http://dx.doi.org/10.3892/mmr.2021.11862 |
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author | Zhang, Xianzhen Ding, Huideng Hu, Gang Lu, Ying |
author_facet | Zhang, Xianzhen Ding, Huideng Hu, Gang Lu, Ying |
author_sort | Zhang, Xianzhen |
collection | PubMed |
description | Previous studies have suggested that the herbal medicine simiaosan has beneficial effects on gouty arthritis (GA), for which conventional Western medicines are insufficient (particularly in cases of multiple episodes). The objective of the present study was to investigate the mechanism by which simiaosan alleviated the symptoms of GA. Sprague-Dawley rat models of acute GA were successfully established, as verified by pathological analyses. Additionally, an NLR family pyrin domain containing 3 (NLRP3) overexpression vector was constructed and a high transfection efficiency was confirmed by reverse transcription PCR. The following five treatment groups were established: i) Normal control; ii) model + saline; iii) model + simiaosan; iv) model + NALP3-overexpressing adenovirus + simiaosan; and v) model + empty vector adenovirus + simiaosan. The samples from mice in each group were subjected to hematoxylin and eosin (H&E) staining for assessing the histopathological changes, enzyme-linked immunosorbent assays for determining IL-1β and TGF-β1 levels and western blotting for evaluating NALP3 expression. H&E staining indicated that simiaosan could reduce the infiltration of inflammatory cells, while NALP3 overexpression aggravated the inflammatory response in tissues. Expression levels of IL-1β, TGF-β1 and NALP3 were significantly higher in the model and the model + NALP3-overexpressing adenovirus + simiaosan groups compared with the normal control group. Levels of IL-1β, TGF-β1 and NALP3 were significantly lower in the model + simiaosan and model + empty vector adenovirus + simiaosan groups compared with the model group. These results indicated that the effects of simiaosan were mediated through NALP3 inhibition. Therefore, the herbal medicine simiaosan was revealed to possess an ability to alleviate the symptoms of GA by regulating the NALP3/IL-1β signaling pathway. |
format | Online Article Text |
id | pubmed-7851828 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | D.A. Spandidos |
record_format | MEDLINE/PubMed |
spelling | pubmed-78518282021-02-17 Simiaosan alleviates the symptoms of gouty arthritis via the NALP3/IL-1β pathway Zhang, Xianzhen Ding, Huideng Hu, Gang Lu, Ying Mol Med Rep Articles Previous studies have suggested that the herbal medicine simiaosan has beneficial effects on gouty arthritis (GA), for which conventional Western medicines are insufficient (particularly in cases of multiple episodes). The objective of the present study was to investigate the mechanism by which simiaosan alleviated the symptoms of GA. Sprague-Dawley rat models of acute GA were successfully established, as verified by pathological analyses. Additionally, an NLR family pyrin domain containing 3 (NLRP3) overexpression vector was constructed and a high transfection efficiency was confirmed by reverse transcription PCR. The following five treatment groups were established: i) Normal control; ii) model + saline; iii) model + simiaosan; iv) model + NALP3-overexpressing adenovirus + simiaosan; and v) model + empty vector adenovirus + simiaosan. The samples from mice in each group were subjected to hematoxylin and eosin (H&E) staining for assessing the histopathological changes, enzyme-linked immunosorbent assays for determining IL-1β and TGF-β1 levels and western blotting for evaluating NALP3 expression. H&E staining indicated that simiaosan could reduce the infiltration of inflammatory cells, while NALP3 overexpression aggravated the inflammatory response in tissues. Expression levels of IL-1β, TGF-β1 and NALP3 were significantly higher in the model and the model + NALP3-overexpressing adenovirus + simiaosan groups compared with the normal control group. Levels of IL-1β, TGF-β1 and NALP3 were significantly lower in the model + simiaosan and model + empty vector adenovirus + simiaosan groups compared with the model group. These results indicated that the effects of simiaosan were mediated through NALP3 inhibition. Therefore, the herbal medicine simiaosan was revealed to possess an ability to alleviate the symptoms of GA by regulating the NALP3/IL-1β signaling pathway. D.A. Spandidos 2021-03 2021-01-24 /pmc/articles/PMC7851828/ /pubmed/33655332 http://dx.doi.org/10.3892/mmr.2021.11862 Text en Copyright: © Zhang et al. This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License (https://creativecommons.org/licenses/by-nc-nd/4.0/) , which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made. |
spellingShingle | Articles Zhang, Xianzhen Ding, Huideng Hu, Gang Lu, Ying Simiaosan alleviates the symptoms of gouty arthritis via the NALP3/IL-1β pathway |
title | Simiaosan alleviates the symptoms of gouty arthritis via the NALP3/IL-1β pathway |
title_full | Simiaosan alleviates the symptoms of gouty arthritis via the NALP3/IL-1β pathway |
title_fullStr | Simiaosan alleviates the symptoms of gouty arthritis via the NALP3/IL-1β pathway |
title_full_unstemmed | Simiaosan alleviates the symptoms of gouty arthritis via the NALP3/IL-1β pathway |
title_short | Simiaosan alleviates the symptoms of gouty arthritis via the NALP3/IL-1β pathway |
title_sort | simiaosan alleviates the symptoms of gouty arthritis via the nalp3/il-1β pathway |
topic | Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7851828/ https://www.ncbi.nlm.nih.gov/pubmed/33655332 http://dx.doi.org/10.3892/mmr.2021.11862 |
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