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Simiaosan alleviates the symptoms of gouty arthritis via the NALP3/IL-1β pathway

Previous studies have suggested that the herbal medicine simiaosan has beneficial effects on gouty arthritis (GA), for which conventional Western medicines are insufficient (particularly in cases of multiple episodes). The objective of the present study was to investigate the mechanism by which simi...

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Autores principales: Zhang, Xianzhen, Ding, Huideng, Hu, Gang, Lu, Ying
Formato: Online Artículo Texto
Lenguaje:English
Publicado: D.A. Spandidos 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7851828/
https://www.ncbi.nlm.nih.gov/pubmed/33655332
http://dx.doi.org/10.3892/mmr.2021.11862
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author Zhang, Xianzhen
Ding, Huideng
Hu, Gang
Lu, Ying
author_facet Zhang, Xianzhen
Ding, Huideng
Hu, Gang
Lu, Ying
author_sort Zhang, Xianzhen
collection PubMed
description Previous studies have suggested that the herbal medicine simiaosan has beneficial effects on gouty arthritis (GA), for which conventional Western medicines are insufficient (particularly in cases of multiple episodes). The objective of the present study was to investigate the mechanism by which simiaosan alleviated the symptoms of GA. Sprague-Dawley rat models of acute GA were successfully established, as verified by pathological analyses. Additionally, an NLR family pyrin domain containing 3 (NLRP3) overexpression vector was constructed and a high transfection efficiency was confirmed by reverse transcription PCR. The following five treatment groups were established: i) Normal control; ii) model + saline; iii) model + simiaosan; iv) model + NALP3-overexpressing adenovirus + simiaosan; and v) model + empty vector adenovirus + simiaosan. The samples from mice in each group were subjected to hematoxylin and eosin (H&E) staining for assessing the histopathological changes, enzyme-linked immunosorbent assays for determining IL-1β and TGF-β1 levels and western blotting for evaluating NALP3 expression. H&E staining indicated that simiaosan could reduce the infiltration of inflammatory cells, while NALP3 overexpression aggravated the inflammatory response in tissues. Expression levels of IL-1β, TGF-β1 and NALP3 were significantly higher in the model and the model + NALP3-overexpressing adenovirus + simiaosan groups compared with the normal control group. Levels of IL-1β, TGF-β1 and NALP3 were significantly lower in the model + simiaosan and model + empty vector adenovirus + simiaosan groups compared with the model group. These results indicated that the effects of simiaosan were mediated through NALP3 inhibition. Therefore, the herbal medicine simiaosan was revealed to possess an ability to alleviate the symptoms of GA by regulating the NALP3/IL-1β signaling pathway.
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spelling pubmed-78518282021-02-17 Simiaosan alleviates the symptoms of gouty arthritis via the NALP3/IL-1β pathway Zhang, Xianzhen Ding, Huideng Hu, Gang Lu, Ying Mol Med Rep Articles Previous studies have suggested that the herbal medicine simiaosan has beneficial effects on gouty arthritis (GA), for which conventional Western medicines are insufficient (particularly in cases of multiple episodes). The objective of the present study was to investigate the mechanism by which simiaosan alleviated the symptoms of GA. Sprague-Dawley rat models of acute GA were successfully established, as verified by pathological analyses. Additionally, an NLR family pyrin domain containing 3 (NLRP3) overexpression vector was constructed and a high transfection efficiency was confirmed by reverse transcription PCR. The following five treatment groups were established: i) Normal control; ii) model + saline; iii) model + simiaosan; iv) model + NALP3-overexpressing adenovirus + simiaosan; and v) model + empty vector adenovirus + simiaosan. The samples from mice in each group were subjected to hematoxylin and eosin (H&E) staining for assessing the histopathological changes, enzyme-linked immunosorbent assays for determining IL-1β and TGF-β1 levels and western blotting for evaluating NALP3 expression. H&E staining indicated that simiaosan could reduce the infiltration of inflammatory cells, while NALP3 overexpression aggravated the inflammatory response in tissues. Expression levels of IL-1β, TGF-β1 and NALP3 were significantly higher in the model and the model + NALP3-overexpressing adenovirus + simiaosan groups compared with the normal control group. Levels of IL-1β, TGF-β1 and NALP3 were significantly lower in the model + simiaosan and model + empty vector adenovirus + simiaosan groups compared with the model group. These results indicated that the effects of simiaosan were mediated through NALP3 inhibition. Therefore, the herbal medicine simiaosan was revealed to possess an ability to alleviate the symptoms of GA by regulating the NALP3/IL-1β signaling pathway. D.A. Spandidos 2021-03 2021-01-24 /pmc/articles/PMC7851828/ /pubmed/33655332 http://dx.doi.org/10.3892/mmr.2021.11862 Text en Copyright: © Zhang et al. This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License (https://creativecommons.org/licenses/by-nc-nd/4.0/) , which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.
spellingShingle Articles
Zhang, Xianzhen
Ding, Huideng
Hu, Gang
Lu, Ying
Simiaosan alleviates the symptoms of gouty arthritis via the NALP3/IL-1β pathway
title Simiaosan alleviates the symptoms of gouty arthritis via the NALP3/IL-1β pathway
title_full Simiaosan alleviates the symptoms of gouty arthritis via the NALP3/IL-1β pathway
title_fullStr Simiaosan alleviates the symptoms of gouty arthritis via the NALP3/IL-1β pathway
title_full_unstemmed Simiaosan alleviates the symptoms of gouty arthritis via the NALP3/IL-1β pathway
title_short Simiaosan alleviates the symptoms of gouty arthritis via the NALP3/IL-1β pathway
title_sort simiaosan alleviates the symptoms of gouty arthritis via the nalp3/il-1β pathway
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7851828/
https://www.ncbi.nlm.nih.gov/pubmed/33655332
http://dx.doi.org/10.3892/mmr.2021.11862
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