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A proteome comparison between human fetal and mature renal extracellular matrix identifies EMILIN1 as a regulator of renal epithelial cell adhesion

Cell-based approaches using tissue engineering and regenerative medicine to replace damaged renal tissue with 3D constructs is a promising emerging therapy for kidney disease. Besides living cells, a template provided by a scaffold based on biomaterials and bioactive factors is needed for successful...

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Autores principales: Louzao-Martinez, Laura, van Dijk, Christian G.M., Xu, Yan Juan, Korn, Amber, Bekker, Nicolaas J., Brouwhuis, Romi, Nicese, Maria Novella, Demmers, Jeroen A.A., Goumans, Marie-José T.H., Masereeuw, Rosalinde, Duncker, Dirk J., Verhaar, Marianne C., Cheng, Caroline
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7852202/
https://www.ncbi.nlm.nih.gov/pubmed/33543009
http://dx.doi.org/10.1016/j.mbplus.2019.100011
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author Louzao-Martinez, Laura
van Dijk, Christian G.M.
Xu, Yan Juan
Korn, Amber
Bekker, Nicolaas J.
Brouwhuis, Romi
Nicese, Maria Novella
Demmers, Jeroen A.A.
Goumans, Marie-José T.H.
Masereeuw, Rosalinde
Duncker, Dirk J.
Verhaar, Marianne C.
Cheng, Caroline
author_facet Louzao-Martinez, Laura
van Dijk, Christian G.M.
Xu, Yan Juan
Korn, Amber
Bekker, Nicolaas J.
Brouwhuis, Romi
Nicese, Maria Novella
Demmers, Jeroen A.A.
Goumans, Marie-José T.H.
Masereeuw, Rosalinde
Duncker, Dirk J.
Verhaar, Marianne C.
Cheng, Caroline
author_sort Louzao-Martinez, Laura
collection PubMed
description Cell-based approaches using tissue engineering and regenerative medicine to replace damaged renal tissue with 3D constructs is a promising emerging therapy for kidney disease. Besides living cells, a template provided by a scaffold based on biomaterials and bioactive factors is needed for successful kidney engineering. Nature's own template for a scaffolding system is the extracellular matrix (ECM). Research has focused on mapping the mature renal ECM; however, the developing fetal ECM matches more the active environment required in 3D renal constructs. Here, we characterized the differences between the human fetal and mature renal ECM using spectrometry-based proteomics of decellularized tissue. We identified 99 different renal ECM proteins of which the majority forms an overlapping core, but also includes proteins enriched in either the fetal or mature ECM. Relative protein quantification showed a significant dominance of EMILIN1 in the fetal ECM. We functionally tested the role of EMILIN1 in the ECM using a novel methodology that permits the reliable anchorage of native cell-secreted ECM to glass coverslips. Depletion of EMILIN1 from the ECM layer using siRNA mediated knock-down technologies does not affect renal epithelial cell growth, but does promote migration. Lack of EMILIN1 in the ECM layer reduces the adhesion strength of renal epithelial cells, shown by a decrease in focal adhesion points and associated stress fibers. We showed in this study the importance of a human renal fetal and mature ECM catalogue for identifying promising ECM components that have high implementation potential in scaffolds for 3D renal constructs.
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spelling pubmed-78522022021-02-03 A proteome comparison between human fetal and mature renal extracellular matrix identifies EMILIN1 as a regulator of renal epithelial cell adhesion Louzao-Martinez, Laura van Dijk, Christian G.M. Xu, Yan Juan Korn, Amber Bekker, Nicolaas J. Brouwhuis, Romi Nicese, Maria Novella Demmers, Jeroen A.A. Goumans, Marie-José T.H. Masereeuw, Rosalinde Duncker, Dirk J. Verhaar, Marianne C. Cheng, Caroline Matrix Biol Plus Article Cell-based approaches using tissue engineering and regenerative medicine to replace damaged renal tissue with 3D constructs is a promising emerging therapy for kidney disease. Besides living cells, a template provided by a scaffold based on biomaterials and bioactive factors is needed for successful kidney engineering. Nature's own template for a scaffolding system is the extracellular matrix (ECM). Research has focused on mapping the mature renal ECM; however, the developing fetal ECM matches more the active environment required in 3D renal constructs. Here, we characterized the differences between the human fetal and mature renal ECM using spectrometry-based proteomics of decellularized tissue. We identified 99 different renal ECM proteins of which the majority forms an overlapping core, but also includes proteins enriched in either the fetal or mature ECM. Relative protein quantification showed a significant dominance of EMILIN1 in the fetal ECM. We functionally tested the role of EMILIN1 in the ECM using a novel methodology that permits the reliable anchorage of native cell-secreted ECM to glass coverslips. Depletion of EMILIN1 from the ECM layer using siRNA mediated knock-down technologies does not affect renal epithelial cell growth, but does promote migration. Lack of EMILIN1 in the ECM layer reduces the adhesion strength of renal epithelial cells, shown by a decrease in focal adhesion points and associated stress fibers. We showed in this study the importance of a human renal fetal and mature ECM catalogue for identifying promising ECM components that have high implementation potential in scaffolds for 3D renal constructs. Elsevier 2019-07-25 /pmc/articles/PMC7852202/ /pubmed/33543009 http://dx.doi.org/10.1016/j.mbplus.2019.100011 Text en © 2019 The Authors http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Article
Louzao-Martinez, Laura
van Dijk, Christian G.M.
Xu, Yan Juan
Korn, Amber
Bekker, Nicolaas J.
Brouwhuis, Romi
Nicese, Maria Novella
Demmers, Jeroen A.A.
Goumans, Marie-José T.H.
Masereeuw, Rosalinde
Duncker, Dirk J.
Verhaar, Marianne C.
Cheng, Caroline
A proteome comparison between human fetal and mature renal extracellular matrix identifies EMILIN1 as a regulator of renal epithelial cell adhesion
title A proteome comparison between human fetal and mature renal extracellular matrix identifies EMILIN1 as a regulator of renal epithelial cell adhesion
title_full A proteome comparison between human fetal and mature renal extracellular matrix identifies EMILIN1 as a regulator of renal epithelial cell adhesion
title_fullStr A proteome comparison between human fetal and mature renal extracellular matrix identifies EMILIN1 as a regulator of renal epithelial cell adhesion
title_full_unstemmed A proteome comparison between human fetal and mature renal extracellular matrix identifies EMILIN1 as a regulator of renal epithelial cell adhesion
title_short A proteome comparison between human fetal and mature renal extracellular matrix identifies EMILIN1 as a regulator of renal epithelial cell adhesion
title_sort proteome comparison between human fetal and mature renal extracellular matrix identifies emilin1 as a regulator of renal epithelial cell adhesion
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7852202/
https://www.ncbi.nlm.nih.gov/pubmed/33543009
http://dx.doi.org/10.1016/j.mbplus.2019.100011
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