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A simple method of drying polyacrylamide slab gels that eliminates cracking
The polyacrylamide slab gel is the most common gel format for analyzing protein samples by electrophoresis. Drying these gels is useful in many biological applications; for example, autoradiography, in which radiolabeled proteins are separated to enable their detection and identification. Dried prot...
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Future Science Ltd
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7852843/ https://www.ncbi.nlm.nih.gov/pubmed/33222512 http://dx.doi.org/10.2144/btn-2020-0117 |
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author | Heda, Ghanshyam D |
author_facet | Heda, Ghanshyam D |
author_sort | Heda, Ghanshyam D |
collection | PubMed |
description | The polyacrylamide slab gel is the most common gel format for analyzing protein samples by electrophoresis. Drying these gels is useful in many biological applications; for example, autoradiography, in which radiolabeled proteins are separated to enable their detection and identification. Dried protein gels can also serve as an ideal method of preserving the gel itself for permanent record-keeping and allowing densitometry at a convenient time. Here I describe a simple and highly reproducible gel-drying method that results in dried gels without the cracks that are frequently encountered with many existing gel-drying methods. |
format | Online Article Text |
id | pubmed-7852843 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Future Science Ltd |
record_format | MEDLINE/PubMed |
spelling | pubmed-78528432021-02-05 A simple method of drying polyacrylamide slab gels that eliminates cracking Heda, Ghanshyam D Biotechniques Benchmark The polyacrylamide slab gel is the most common gel format for analyzing protein samples by electrophoresis. Drying these gels is useful in many biological applications; for example, autoradiography, in which radiolabeled proteins are separated to enable their detection and identification. Dried protein gels can also serve as an ideal method of preserving the gel itself for permanent record-keeping and allowing densitometry at a convenient time. Here I describe a simple and highly reproducible gel-drying method that results in dried gels without the cracks that are frequently encountered with many existing gel-drying methods. Future Science Ltd 2020-11-23 2020-10 /pmc/articles/PMC7852843/ /pubmed/33222512 http://dx.doi.org/10.2144/btn-2020-0117 Text en © 2020 Ghanshyam D Heda This work is licensed under the Attribution-NonCommercial-NoDerivatives 4.0 Unported License (http://creativecommons.org/licenses/by-nc-nd/4.0/) |
spellingShingle | Benchmark Heda, Ghanshyam D A simple method of drying polyacrylamide slab gels that eliminates cracking |
title | A simple method of drying polyacrylamide slab gels that eliminates cracking |
title_full | A simple method of drying polyacrylamide slab gels that eliminates cracking |
title_fullStr | A simple method of drying polyacrylamide slab gels that eliminates cracking |
title_full_unstemmed | A simple method of drying polyacrylamide slab gels that eliminates cracking |
title_short | A simple method of drying polyacrylamide slab gels that eliminates cracking |
title_sort | simple method of drying polyacrylamide slab gels that eliminates cracking |
topic | Benchmark |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7852843/ https://www.ncbi.nlm.nih.gov/pubmed/33222512 http://dx.doi.org/10.2144/btn-2020-0117 |
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