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A simple method of drying polyacrylamide slab gels that eliminates cracking

The polyacrylamide slab gel is the most common gel format for analyzing protein samples by electrophoresis. Drying these gels is useful in many biological applications; for example, autoradiography, in which radiolabeled proteins are separated to enable their detection and identification. Dried prot...

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Detalles Bibliográficos
Autor principal: Heda, Ghanshyam D
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Future Science Ltd 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7852843/
https://www.ncbi.nlm.nih.gov/pubmed/33222512
http://dx.doi.org/10.2144/btn-2020-0117
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author Heda, Ghanshyam D
author_facet Heda, Ghanshyam D
author_sort Heda, Ghanshyam D
collection PubMed
description The polyacrylamide slab gel is the most common gel format for analyzing protein samples by electrophoresis. Drying these gels is useful in many biological applications; for example, autoradiography, in which radiolabeled proteins are separated to enable their detection and identification. Dried protein gels can also serve as an ideal method of preserving the gel itself for permanent record-keeping and allowing densitometry at a convenient time. Here I describe a simple and highly reproducible gel-drying method that results in dried gels without the cracks that are frequently encountered with many existing gel-drying methods.
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spelling pubmed-78528432021-02-05 A simple method of drying polyacrylamide slab gels that eliminates cracking Heda, Ghanshyam D Biotechniques Benchmark The polyacrylamide slab gel is the most common gel format for analyzing protein samples by electrophoresis. Drying these gels is useful in many biological applications; for example, autoradiography, in which radiolabeled proteins are separated to enable their detection and identification. Dried protein gels can also serve as an ideal method of preserving the gel itself for permanent record-keeping and allowing densitometry at a convenient time. Here I describe a simple and highly reproducible gel-drying method that results in dried gels without the cracks that are frequently encountered with many existing gel-drying methods. Future Science Ltd 2020-11-23 2020-10 /pmc/articles/PMC7852843/ /pubmed/33222512 http://dx.doi.org/10.2144/btn-2020-0117 Text en © 2020 Ghanshyam D Heda This work is licensed under the Attribution-NonCommercial-NoDerivatives 4.0 Unported License (http://creativecommons.org/licenses/by-nc-nd/4.0/)
spellingShingle Benchmark
Heda, Ghanshyam D
A simple method of drying polyacrylamide slab gels that eliminates cracking
title A simple method of drying polyacrylamide slab gels that eliminates cracking
title_full A simple method of drying polyacrylamide slab gels that eliminates cracking
title_fullStr A simple method of drying polyacrylamide slab gels that eliminates cracking
title_full_unstemmed A simple method of drying polyacrylamide slab gels that eliminates cracking
title_short A simple method of drying polyacrylamide slab gels that eliminates cracking
title_sort simple method of drying polyacrylamide slab gels that eliminates cracking
topic Benchmark
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7852843/
https://www.ncbi.nlm.nih.gov/pubmed/33222512
http://dx.doi.org/10.2144/btn-2020-0117
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