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The initial inoculation ratio regulates bacterial coculture interactions and metabolic capacity

Coculture is an important model system in microbial ecology studies. As a key experimental parameter, the initial inoculation ratio has a crucial impact on the results of the coculture system. However, such an effect has never been investigated under multiple niche conditions. In this study, we esta...

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Autores principales: Gao, Chun-Hui, Cao, Hui, Cai, Peng, Sørensen, Søren J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7852870/
https://www.ncbi.nlm.nih.gov/pubmed/32887945
http://dx.doi.org/10.1038/s41396-020-00751-7
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author Gao, Chun-Hui
Cao, Hui
Cai, Peng
Sørensen, Søren J.
author_facet Gao, Chun-Hui
Cao, Hui
Cai, Peng
Sørensen, Søren J.
author_sort Gao, Chun-Hui
collection PubMed
description Coculture is an important model system in microbial ecology studies. As a key experimental parameter, the initial inoculation ratio has a crucial impact on the results of the coculture system. However, such an effect has never been investigated under multiple niche conditions. In this study, we established a simple coculture system with two model bacteria in various carbon sources and investigated the influence of initial inoculum ratios of 1:1000 to 1000:1 on community structure, function, and bacterial interaction. We found that the final ratio of the cocultures with different initial inoculum ratios differed in approximately five-sixths of the carbon sources, suggesting that the final ratio is highly dependent on the initial inoculum ratio, while the carbon source preferences of bacteria could not predict the final ratio of cocultures. Furthermore, we found that the initial ratio could regulate the metabolic capacity of the coculture, as only cocultures with initial ratios of 1:1 and 1000:1 gained high capacity on 14 specific carbon sources. The underlying reason may be that the pattern of species interaction is changed by the initial ratio. In conclusion, we showed that the initial ratio can induce emergent properties in coculture. These findings suggest that the initial ratio not only impacts the reproducibility of coculture experiments but also can influence our understanding of generic microbial ecology.
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spelling pubmed-78528702021-02-08 The initial inoculation ratio regulates bacterial coculture interactions and metabolic capacity Gao, Chun-Hui Cao, Hui Cai, Peng Sørensen, Søren J. ISME J Article Coculture is an important model system in microbial ecology studies. As a key experimental parameter, the initial inoculation ratio has a crucial impact on the results of the coculture system. However, such an effect has never been investigated under multiple niche conditions. In this study, we established a simple coculture system with two model bacteria in various carbon sources and investigated the influence of initial inoculum ratios of 1:1000 to 1000:1 on community structure, function, and bacterial interaction. We found that the final ratio of the cocultures with different initial inoculum ratios differed in approximately five-sixths of the carbon sources, suggesting that the final ratio is highly dependent on the initial inoculum ratio, while the carbon source preferences of bacteria could not predict the final ratio of cocultures. Furthermore, we found that the initial ratio could regulate the metabolic capacity of the coculture, as only cocultures with initial ratios of 1:1 and 1000:1 gained high capacity on 14 specific carbon sources. The underlying reason may be that the pattern of species interaction is changed by the initial ratio. In conclusion, we showed that the initial ratio can induce emergent properties in coculture. These findings suggest that the initial ratio not only impacts the reproducibility of coculture experiments but also can influence our understanding of generic microbial ecology. Nature Publishing Group UK 2020-09-04 2021-01 /pmc/articles/PMC7852870/ /pubmed/32887945 http://dx.doi.org/10.1038/s41396-020-00751-7 Text en © The Author(s) 2020 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Gao, Chun-Hui
Cao, Hui
Cai, Peng
Sørensen, Søren J.
The initial inoculation ratio regulates bacterial coculture interactions and metabolic capacity
title The initial inoculation ratio regulates bacterial coculture interactions and metabolic capacity
title_full The initial inoculation ratio regulates bacterial coculture interactions and metabolic capacity
title_fullStr The initial inoculation ratio regulates bacterial coculture interactions and metabolic capacity
title_full_unstemmed The initial inoculation ratio regulates bacterial coculture interactions and metabolic capacity
title_short The initial inoculation ratio regulates bacterial coculture interactions and metabolic capacity
title_sort initial inoculation ratio regulates bacterial coculture interactions and metabolic capacity
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7852870/
https://www.ncbi.nlm.nih.gov/pubmed/32887945
http://dx.doi.org/10.1038/s41396-020-00751-7
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