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Quantitative proteomic analysis identified differentially expressed proteins with tail/rump fat deposition in Chinese thin- and fat-tailed lambs

Tail adipose as one of the important functional tissues can enhance hazardous environments tolerance for sheep. The objective of this study was to gain insight into the underlying development mechanisms of this trait. A quantitative analysis of protein abundance in ovine tail/rump adipose tissue was...

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Autores principales: Han, Jilong, Guo, Tingting, Yue, Yaojing, Lu, Zengkui, Liu, Jianbin, Yuan, Chao, Niu, Chune, Yang, Min, Yang, Bohui
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7853479/
https://www.ncbi.nlm.nih.gov/pubmed/33529214
http://dx.doi.org/10.1371/journal.pone.0246279
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author Han, Jilong
Guo, Tingting
Yue, Yaojing
Lu, Zengkui
Liu, Jianbin
Yuan, Chao
Niu, Chune
Yang, Min
Yang, Bohui
author_facet Han, Jilong
Guo, Tingting
Yue, Yaojing
Lu, Zengkui
Liu, Jianbin
Yuan, Chao
Niu, Chune
Yang, Min
Yang, Bohui
author_sort Han, Jilong
collection PubMed
description Tail adipose as one of the important functional tissues can enhance hazardous environments tolerance for sheep. The objective of this study was to gain insight into the underlying development mechanisms of this trait. A quantitative analysis of protein abundance in ovine tail/rump adipose tissue was performed between Chinese local fat- (Kazakh, Hu and Lanzhou) and thin-tailed (Alpine Merino, Tibetan) sheep in the present study by using lable-free approach. Results showed that 3400 proteins were identified in the five breeds, and 804 were differentially expressed proteins, including 638 up regulated proteins and 83 down regulated proteins in the tail adipose tissues between fat- and thin-tailed sheep, and 8 clusters were distinguished for all the DEPs’ expression patterns. The differentially expressed proteins are mainly associated with metabolism pathways and peroxisome proliferator activated receptor signaling pathway. Furthermore, the proteomics results were validated by quantitative real-time PCR and Western Blot. Our research has also suggested that the up-regulated proteins ACSL1, HSD17β4, FABP4 in the tail adipose tissue might contribute to tail fat deposition by facilitating the proliferation of adipocytes and fat accumulation in tail/rump of sheep. Particularly, FABP4 highly expressed in the fat-tail will play an important role for tail fat deposition. Our study might provide a novel view to understanding fat accumulation in special parts of the body in sheep and other animals.
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spelling pubmed-78534792021-02-09 Quantitative proteomic analysis identified differentially expressed proteins with tail/rump fat deposition in Chinese thin- and fat-tailed lambs Han, Jilong Guo, Tingting Yue, Yaojing Lu, Zengkui Liu, Jianbin Yuan, Chao Niu, Chune Yang, Min Yang, Bohui PLoS One Research Article Tail adipose as one of the important functional tissues can enhance hazardous environments tolerance for sheep. The objective of this study was to gain insight into the underlying development mechanisms of this trait. A quantitative analysis of protein abundance in ovine tail/rump adipose tissue was performed between Chinese local fat- (Kazakh, Hu and Lanzhou) and thin-tailed (Alpine Merino, Tibetan) sheep in the present study by using lable-free approach. Results showed that 3400 proteins were identified in the five breeds, and 804 were differentially expressed proteins, including 638 up regulated proteins and 83 down regulated proteins in the tail adipose tissues between fat- and thin-tailed sheep, and 8 clusters were distinguished for all the DEPs’ expression patterns. The differentially expressed proteins are mainly associated with metabolism pathways and peroxisome proliferator activated receptor signaling pathway. Furthermore, the proteomics results were validated by quantitative real-time PCR and Western Blot. Our research has also suggested that the up-regulated proteins ACSL1, HSD17β4, FABP4 in the tail adipose tissue might contribute to tail fat deposition by facilitating the proliferation of adipocytes and fat accumulation in tail/rump of sheep. Particularly, FABP4 highly expressed in the fat-tail will play an important role for tail fat deposition. Our study might provide a novel view to understanding fat accumulation in special parts of the body in sheep and other animals. Public Library of Science 2021-02-02 /pmc/articles/PMC7853479/ /pubmed/33529214 http://dx.doi.org/10.1371/journal.pone.0246279 Text en © 2021 Han et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Han, Jilong
Guo, Tingting
Yue, Yaojing
Lu, Zengkui
Liu, Jianbin
Yuan, Chao
Niu, Chune
Yang, Min
Yang, Bohui
Quantitative proteomic analysis identified differentially expressed proteins with tail/rump fat deposition in Chinese thin- and fat-tailed lambs
title Quantitative proteomic analysis identified differentially expressed proteins with tail/rump fat deposition in Chinese thin- and fat-tailed lambs
title_full Quantitative proteomic analysis identified differentially expressed proteins with tail/rump fat deposition in Chinese thin- and fat-tailed lambs
title_fullStr Quantitative proteomic analysis identified differentially expressed proteins with tail/rump fat deposition in Chinese thin- and fat-tailed lambs
title_full_unstemmed Quantitative proteomic analysis identified differentially expressed proteins with tail/rump fat deposition in Chinese thin- and fat-tailed lambs
title_short Quantitative proteomic analysis identified differentially expressed proteins with tail/rump fat deposition in Chinese thin- and fat-tailed lambs
title_sort quantitative proteomic analysis identified differentially expressed proteins with tail/rump fat deposition in chinese thin- and fat-tailed lambs
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7853479/
https://www.ncbi.nlm.nih.gov/pubmed/33529214
http://dx.doi.org/10.1371/journal.pone.0246279
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