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A new genome allows the identification of genes associated with natural variation in aluminium tolerance in Brachiaria grasses

Toxic concentrations of aluminium cations and low phosphorus availability are the main yield-limiting factors in acidic soils, which represent half of the potentially available arable land. Brachiaria grasses, which are commonly sown as forage in the tropics because of their resilience and low deman...

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Autores principales: Worthington, Margaret, Perez, Juan Guillermo, Mussurova, Saule, Silva-Cordoba, Alexander, Castiblanco, Valheria, Cardoso Arango, Juan Andres, Jones, Charlotte, Fernandez-Fuentes, Narcis, Skot, Leif, Dyer, Sarah, Tohme, Joe, Di Palma, Federica, Arango, Jacobo, Armstead, Ian, De Vega, Jose J
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7853602/
https://www.ncbi.nlm.nih.gov/pubmed/33064149
http://dx.doi.org/10.1093/jxb/eraa469
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author Worthington, Margaret
Perez, Juan Guillermo
Mussurova, Saule
Silva-Cordoba, Alexander
Castiblanco, Valheria
Cardoso Arango, Juan Andres
Jones, Charlotte
Fernandez-Fuentes, Narcis
Skot, Leif
Dyer, Sarah
Tohme, Joe
Di Palma, Federica
Arango, Jacobo
Armstead, Ian
De Vega, Jose J
author_facet Worthington, Margaret
Perez, Juan Guillermo
Mussurova, Saule
Silva-Cordoba, Alexander
Castiblanco, Valheria
Cardoso Arango, Juan Andres
Jones, Charlotte
Fernandez-Fuentes, Narcis
Skot, Leif
Dyer, Sarah
Tohme, Joe
Di Palma, Federica
Arango, Jacobo
Armstead, Ian
De Vega, Jose J
author_sort Worthington, Margaret
collection PubMed
description Toxic concentrations of aluminium cations and low phosphorus availability are the main yield-limiting factors in acidic soils, which represent half of the potentially available arable land. Brachiaria grasses, which are commonly sown as forage in the tropics because of their resilience and low demand for nutrients, show greater tolerance to high concentrations of aluminium cations (Al(3+)) than most other grass crops. In this work, we explored the natural variation in tolerance to Al(3+) between high and low tolerant Brachiaria species and characterized their transcriptional differences during stress. We identified three QTLs (quantitative trait loci) associated with root vigour during Al(3+) stress in their hybrid progeny. By integrating these results with a new Brachiaria reference genome, we identified 30 genes putatively responsible for Al(3+) tolerance in Brachiaria. We observed differential expression during stress of genes involved in RNA translation, response signalling, cell wall composition, and vesicle location homologous to aluminium-induced proteins involved in limiting uptake or localizing the toxin. However, there was limited regulation of malate transporters in Brachiaria, which suggests that exudation of organic acids and other external tolerance mechanisms, common in other grasses, might not be relevant in Brachiaria. The contrasting regulation of RNA translation and response signalling suggests that response timing is critical in high Al(3+)-tolerant Brachiaria.
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spelling pubmed-78536022021-02-04 A new genome allows the identification of genes associated with natural variation in aluminium tolerance in Brachiaria grasses Worthington, Margaret Perez, Juan Guillermo Mussurova, Saule Silva-Cordoba, Alexander Castiblanco, Valheria Cardoso Arango, Juan Andres Jones, Charlotte Fernandez-Fuentes, Narcis Skot, Leif Dyer, Sarah Tohme, Joe Di Palma, Federica Arango, Jacobo Armstead, Ian De Vega, Jose J J Exp Bot Research Papers Toxic concentrations of aluminium cations and low phosphorus availability are the main yield-limiting factors in acidic soils, which represent half of the potentially available arable land. Brachiaria grasses, which are commonly sown as forage in the tropics because of their resilience and low demand for nutrients, show greater tolerance to high concentrations of aluminium cations (Al(3+)) than most other grass crops. In this work, we explored the natural variation in tolerance to Al(3+) between high and low tolerant Brachiaria species and characterized their transcriptional differences during stress. We identified three QTLs (quantitative trait loci) associated with root vigour during Al(3+) stress in their hybrid progeny. By integrating these results with a new Brachiaria reference genome, we identified 30 genes putatively responsible for Al(3+) tolerance in Brachiaria. We observed differential expression during stress of genes involved in RNA translation, response signalling, cell wall composition, and vesicle location homologous to aluminium-induced proteins involved in limiting uptake or localizing the toxin. However, there was limited regulation of malate transporters in Brachiaria, which suggests that exudation of organic acids and other external tolerance mechanisms, common in other grasses, might not be relevant in Brachiaria. The contrasting regulation of RNA translation and response signalling suggests that response timing is critical in high Al(3+)-tolerant Brachiaria. Oxford University Press 2020-10-16 /pmc/articles/PMC7853602/ /pubmed/33064149 http://dx.doi.org/10.1093/jxb/eraa469 Text en © The Author(s) 2020. Published by Oxford University Press on behalf of the Society for Experimental Biology. http://creativecommons.org/licenses/by/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Papers
Worthington, Margaret
Perez, Juan Guillermo
Mussurova, Saule
Silva-Cordoba, Alexander
Castiblanco, Valheria
Cardoso Arango, Juan Andres
Jones, Charlotte
Fernandez-Fuentes, Narcis
Skot, Leif
Dyer, Sarah
Tohme, Joe
Di Palma, Federica
Arango, Jacobo
Armstead, Ian
De Vega, Jose J
A new genome allows the identification of genes associated with natural variation in aluminium tolerance in Brachiaria grasses
title A new genome allows the identification of genes associated with natural variation in aluminium tolerance in Brachiaria grasses
title_full A new genome allows the identification of genes associated with natural variation in aluminium tolerance in Brachiaria grasses
title_fullStr A new genome allows the identification of genes associated with natural variation in aluminium tolerance in Brachiaria grasses
title_full_unstemmed A new genome allows the identification of genes associated with natural variation in aluminium tolerance in Brachiaria grasses
title_short A new genome allows the identification of genes associated with natural variation in aluminium tolerance in Brachiaria grasses
title_sort new genome allows the identification of genes associated with natural variation in aluminium tolerance in brachiaria grasses
topic Research Papers
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7853602/
https://www.ncbi.nlm.nih.gov/pubmed/33064149
http://dx.doi.org/10.1093/jxb/eraa469
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