Cargando…
The effect of dexmedetomidine on gastric ischemia reperfusion injury in rats. Biochemical and histopathological evaluation
PURPOSE: To evaluate the protective effect of dexmedetomidine on gastric injury induced by ischemia reperfusion (I/R) in rats. METHODS: A total of 18 male albino Wistar rats were divided groups as: gastric ischemia reperfusion (GIR), gastric ischemia reperfusion and 50 μg/kg dexmedetomidine (DGIR) a...
Autores principales: | , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Sociedade Brasileira para o Desenvolvimento da Pesquisa em
Cirurgia
2021
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7853698/ https://www.ncbi.nlm.nih.gov/pubmed/33533828 http://dx.doi.org/10.1590/ACB360104 |
_version_ | 1783646014150803456 |
---|---|
author | Kuyrukluyildiz, Ufuk Delen, Leman Acun Onk, Didem Yazici, Gulce Naz Gulaboglu, Mine Suleyman, Halis |
author_facet | Kuyrukluyildiz, Ufuk Delen, Leman Acun Onk, Didem Yazici, Gulce Naz Gulaboglu, Mine Suleyman, Halis |
author_sort | Kuyrukluyildiz, Ufuk |
collection | PubMed |
description | PURPOSE: To evaluate the protective effect of dexmedetomidine on gastric injury induced by ischemia reperfusion (I/R) in rats. METHODS: A total of 18 male albino Wistar rats were divided groups as: gastric ischemia reperfusion (GIR), gastric ischemia reperfusion and 50 μg/kg dexmedetomidine (DGIR) and sham operation (HG) group. After the third hour of reperfusion, the biochemical and histopathological examinations were performed on the removed stomach tissue. RESULTS: Malondialdehyde (MDA) and myeloperoxidase (MPO) levels were found to be significantly higher in GIR compared to HG (p < 0.05). A statistically significant decrease was observed at the DGIR compared to the GIR for oxidants levels. Total glutathione (tGSH) and superoxide dismutase (SOD) levels were statistically significantly decreased at the GIR, and antioxidants levels were found to be significantly higher in the DGIR (p < 0.05) There was no significant difference between HG and DGIR in terms of SOD (p = 0.097). The DGIRs’ epitheliums, glands and vascular structures were close to normal histological formation. CONCLUSIONS: Dexmedetomidine is found to prevent oxidative damage on the stomach by increasing the antioxidant effect. These results indicate that dexmedetomidine may be useful in the treatment of ischemia-reperfusion-related gastric damage. |
format | Online Article Text |
id | pubmed-7853698 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Sociedade Brasileira para o Desenvolvimento da Pesquisa em
Cirurgia |
record_format | MEDLINE/PubMed |
spelling | pubmed-78536982021-02-09 The effect of dexmedetomidine on gastric ischemia reperfusion injury in rats. Biochemical and histopathological evaluation Kuyrukluyildiz, Ufuk Delen, Leman Acun Onk, Didem Yazici, Gulce Naz Gulaboglu, Mine Suleyman, Halis Acta Cir Bras Original Article PURPOSE: To evaluate the protective effect of dexmedetomidine on gastric injury induced by ischemia reperfusion (I/R) in rats. METHODS: A total of 18 male albino Wistar rats were divided groups as: gastric ischemia reperfusion (GIR), gastric ischemia reperfusion and 50 μg/kg dexmedetomidine (DGIR) and sham operation (HG) group. After the third hour of reperfusion, the biochemical and histopathological examinations were performed on the removed stomach tissue. RESULTS: Malondialdehyde (MDA) and myeloperoxidase (MPO) levels were found to be significantly higher in GIR compared to HG (p < 0.05). A statistically significant decrease was observed at the DGIR compared to the GIR for oxidants levels. Total glutathione (tGSH) and superoxide dismutase (SOD) levels were statistically significantly decreased at the GIR, and antioxidants levels were found to be significantly higher in the DGIR (p < 0.05) There was no significant difference between HG and DGIR in terms of SOD (p = 0.097). The DGIRs’ epitheliums, glands and vascular structures were close to normal histological formation. CONCLUSIONS: Dexmedetomidine is found to prevent oxidative damage on the stomach by increasing the antioxidant effect. These results indicate that dexmedetomidine may be useful in the treatment of ischemia-reperfusion-related gastric damage. Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia 2021-02-01 /pmc/articles/PMC7853698/ /pubmed/33533828 http://dx.doi.org/10.1590/ACB360104 Text en https://creativecommons.org/licenses/by/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Article Kuyrukluyildiz, Ufuk Delen, Leman Acun Onk, Didem Yazici, Gulce Naz Gulaboglu, Mine Suleyman, Halis The effect of dexmedetomidine on gastric ischemia reperfusion injury in rats. Biochemical and histopathological evaluation |
title | The effect of dexmedetomidine on gastric ischemia reperfusion injury
in rats. Biochemical and histopathological evaluation |
title_full | The effect of dexmedetomidine on gastric ischemia reperfusion injury
in rats. Biochemical and histopathological evaluation |
title_fullStr | The effect of dexmedetomidine on gastric ischemia reperfusion injury
in rats. Biochemical and histopathological evaluation |
title_full_unstemmed | The effect of dexmedetomidine on gastric ischemia reperfusion injury
in rats. Biochemical and histopathological evaluation |
title_short | The effect of dexmedetomidine on gastric ischemia reperfusion injury
in rats. Biochemical and histopathological evaluation |
title_sort | effect of dexmedetomidine on gastric ischemia reperfusion injury
in rats. biochemical and histopathological evaluation |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7853698/ https://www.ncbi.nlm.nih.gov/pubmed/33533828 http://dx.doi.org/10.1590/ACB360104 |
work_keys_str_mv | AT kuyrukluyildizufuk theeffectofdexmedetomidineongastricischemiareperfusioninjuryinratsbiochemicalandhistopathologicalevaluation AT delenlemanacun theeffectofdexmedetomidineongastricischemiareperfusioninjuryinratsbiochemicalandhistopathologicalevaluation AT onkdidem theeffectofdexmedetomidineongastricischemiareperfusioninjuryinratsbiochemicalandhistopathologicalevaluation AT yazicigulcenaz theeffectofdexmedetomidineongastricischemiareperfusioninjuryinratsbiochemicalandhistopathologicalevaluation AT gulaboglumine theeffectofdexmedetomidineongastricischemiareperfusioninjuryinratsbiochemicalandhistopathologicalevaluation AT suleymanhalis theeffectofdexmedetomidineongastricischemiareperfusioninjuryinratsbiochemicalandhistopathologicalevaluation AT kuyrukluyildizufuk effectofdexmedetomidineongastricischemiareperfusioninjuryinratsbiochemicalandhistopathologicalevaluation AT delenlemanacun effectofdexmedetomidineongastricischemiareperfusioninjuryinratsbiochemicalandhistopathologicalevaluation AT onkdidem effectofdexmedetomidineongastricischemiareperfusioninjuryinratsbiochemicalandhistopathologicalevaluation AT yazicigulcenaz effectofdexmedetomidineongastricischemiareperfusioninjuryinratsbiochemicalandhistopathologicalevaluation AT gulaboglumine effectofdexmedetomidineongastricischemiareperfusioninjuryinratsbiochemicalandhistopathologicalevaluation AT suleymanhalis effectofdexmedetomidineongastricischemiareperfusioninjuryinratsbiochemicalandhistopathologicalevaluation |