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Quantification of lipid bodies in monocytes from patients with periodontitis

OBJECTIVES: For the first time in the history of periodontics, the production of lipid bodies by monocytes was assessed from blood of patients with periodontitis in comparison to systemically healthy individuals. The purpose of this study was to compare the lipid body frequency within monocytes betw...

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Autores principales: Naiff, Priscilla F., Kuckelhaus, Selma A. S., Corazza, Danilo, Leite, Luciana M., Couto, Shirley, deOliveira, Mariangela S., Santiago, Luander M., Silva, Larissa F., Oliveira, Laudimar A., Grisi, Daniela C., Carneiro, Valeria M. A., Guimarães, Maria do C. M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7853883/
https://www.ncbi.nlm.nih.gov/pubmed/33188556
http://dx.doi.org/10.1002/cre2.340
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author Naiff, Priscilla F.
Kuckelhaus, Selma A. S.
Corazza, Danilo
Leite, Luciana M.
Couto, Shirley
deOliveira, Mariangela S.
Santiago, Luander M.
Silva, Larissa F.
Oliveira, Laudimar A.
Grisi, Daniela C.
Carneiro, Valeria M. A.
Guimarães, Maria do C. M.
author_facet Naiff, Priscilla F.
Kuckelhaus, Selma A. S.
Corazza, Danilo
Leite, Luciana M.
Couto, Shirley
deOliveira, Mariangela S.
Santiago, Luander M.
Silva, Larissa F.
Oliveira, Laudimar A.
Grisi, Daniela C.
Carneiro, Valeria M. A.
Guimarães, Maria do C. M.
author_sort Naiff, Priscilla F.
collection PubMed
description OBJECTIVES: For the first time in the history of periodontics, the production of lipid bodies by monocytes was assessed from blood of patients with periodontitis in comparison to systemically healthy individuals. The purpose of this study was to compare the lipid body frequency within monocytes between healthy patients and those with periodontal disease. MATERIALS AND METHODS: A total of 30 participants (11 males and 19 females), were divided between orally healthy control subjects (C, n = 16) and periodontitis subjects (P, n = 14), in a cross‐sectional study. Both groups were systemically healthy. The following clinical periodontal parameters were assessed: probing depth, clinical attachment level, visible plaque index and gingival bleeding on probing index. Blood samples were collected to obtain monocytes containing lipid bodies, which were analyzed by light microscopy. RESULTS: The periodontitis group demonstrated a higher corpuscular index than the control group (nonopsonized p = .0296 or opsonized p = .0459; Mann–Whitney). The frequency of monocyte cells containing lipid bodies (basal p = .0147, opsonized p = .0084 or nonopsonized, p = .026; Mann–Whitney) was also higher compared to those observed in healthy individuals. CONCLUSIONS: The data suggest that periodontitis may contribute to a higher production of lipid bodies. It was also hypothesized that a major production of lipid bodies by monocytes in severe periodontitis, compared to orally healthy subjects, could interfere with the innate immune response or represents a higher reservoir of cholesterol esters within macrophages and a major risk to systemic implications, such as atherosclerosis.
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spelling pubmed-78538832021-02-05 Quantification of lipid bodies in monocytes from patients with periodontitis Naiff, Priscilla F. Kuckelhaus, Selma A. S. Corazza, Danilo Leite, Luciana M. Couto, Shirley deOliveira, Mariangela S. Santiago, Luander M. Silva, Larissa F. Oliveira, Laudimar A. Grisi, Daniela C. Carneiro, Valeria M. A. Guimarães, Maria do C. M. Clin Exp Dent Res Original Articles OBJECTIVES: For the first time in the history of periodontics, the production of lipid bodies by monocytes was assessed from blood of patients with periodontitis in comparison to systemically healthy individuals. The purpose of this study was to compare the lipid body frequency within monocytes between healthy patients and those with periodontal disease. MATERIALS AND METHODS: A total of 30 participants (11 males and 19 females), were divided between orally healthy control subjects (C, n = 16) and periodontitis subjects (P, n = 14), in a cross‐sectional study. Both groups were systemically healthy. The following clinical periodontal parameters were assessed: probing depth, clinical attachment level, visible plaque index and gingival bleeding on probing index. Blood samples were collected to obtain monocytes containing lipid bodies, which were analyzed by light microscopy. RESULTS: The periodontitis group demonstrated a higher corpuscular index than the control group (nonopsonized p = .0296 or opsonized p = .0459; Mann–Whitney). The frequency of monocyte cells containing lipid bodies (basal p = .0147, opsonized p = .0084 or nonopsonized, p = .026; Mann–Whitney) was also higher compared to those observed in healthy individuals. CONCLUSIONS: The data suggest that periodontitis may contribute to a higher production of lipid bodies. It was also hypothesized that a major production of lipid bodies by monocytes in severe periodontitis, compared to orally healthy subjects, could interfere with the innate immune response or represents a higher reservoir of cholesterol esters within macrophages and a major risk to systemic implications, such as atherosclerosis. John Wiley and Sons Inc. 2020-11-14 /pmc/articles/PMC7853883/ /pubmed/33188556 http://dx.doi.org/10.1002/cre2.340 Text en © 2020 The Authors. Clinical and Experimental Dental Research published by John Wiley & Sons Ltd. This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Articles
Naiff, Priscilla F.
Kuckelhaus, Selma A. S.
Corazza, Danilo
Leite, Luciana M.
Couto, Shirley
deOliveira, Mariangela S.
Santiago, Luander M.
Silva, Larissa F.
Oliveira, Laudimar A.
Grisi, Daniela C.
Carneiro, Valeria M. A.
Guimarães, Maria do C. M.
Quantification of lipid bodies in monocytes from patients with periodontitis
title Quantification of lipid bodies in monocytes from patients with periodontitis
title_full Quantification of lipid bodies in monocytes from patients with periodontitis
title_fullStr Quantification of lipid bodies in monocytes from patients with periodontitis
title_full_unstemmed Quantification of lipid bodies in monocytes from patients with periodontitis
title_short Quantification of lipid bodies in monocytes from patients with periodontitis
title_sort quantification of lipid bodies in monocytes from patients with periodontitis
topic Original Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7853883/
https://www.ncbi.nlm.nih.gov/pubmed/33188556
http://dx.doi.org/10.1002/cre2.340
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