Production and Purification of Dengue Virus-like Particles from COS-1 Cells

Non-infectious virus-like particles (VLPs) containing dengue virus (DENV) pre-membrane (prM) and envelope (E) proteins have been demonstrated to be highly immunogenic and can be used as a potential vaccine candidate as well as a tool for serodiagnostic assays. Successful application of VLPs requires...

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Detalles Bibliográficos
Autores principales: Galula, Jedhan Ucat, Chang, Gwong-Jen J., Chao, Day-Yu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Bio-Protocol 2019
Materias:
Methods Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7854098/
https://www.ncbi.nlm.nih.gov/pubmed/33654796
http://dx.doi.org/10.21769/BioProtoc.3280
Descripción
Sumario:Non-infectious virus-like particles (VLPs) containing dengue virus (DENV) pre-membrane (prM) and envelope (E) proteins have been demonstrated to be highly immunogenic and can be used as a potential vaccine candidate as well as a tool for serodiagnostic assays. Successful application of VLPs requires abundant, and high-purity production methods. Here, we describe a robust protocol for producing DENV VLPs from transiently-transformed or stable COS-1 cells and further provide an easily adaptable antigen purification method by sucrose gradient centrifugation.

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