Production and Purification of Dengue Virus-like Particles from COS-1 Cells

Non-infectious virus-like particles (VLPs) containing dengue virus (DENV) pre-membrane (prM) and envelope (E) proteins have been demonstrated to be highly immunogenic and can be used as a potential vaccine candidate as well as a tool for serodiagnostic assays. Successful application of VLPs requires...

Descripción completa

Detalles Bibliográficos
Autores principales: Galula, Jedhan Ucat, Chang, Gwong-Jen J., Chao, Day-Yu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Bio-Protocol 2019
Materias:
Methods Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7854098/
https://www.ncbi.nlm.nih.gov/pubmed/33654796
http://dx.doi.org/10.21769/BioProtoc.3280
_version_ 1783646057372057600
author Galula, Jedhan Ucat
Chang, Gwong-Jen J.
Chao, Day-Yu
author_facet Galula, Jedhan Ucat
Chang, Gwong-Jen J.
Chao, Day-Yu
author_sort Galula, Jedhan Ucat
collection PubMed
description Non-infectious virus-like particles (VLPs) containing dengue virus (DENV) pre-membrane (prM) and envelope (E) proteins have been demonstrated to be highly immunogenic and can be used as a potential vaccine candidate as well as a tool for serodiagnostic assays. Successful application of VLPs requires abundant, and high-purity production methods. Here, we describe a robust protocol for producing DENV VLPs from transiently-transformed or stable COS-1 cells and further provide an easily adaptable antigen purification method by sucrose gradient centrifugation.
format Online
Article
Text
id pubmed-7854098
institution National Center for Biotechnology Information
language English
publishDate 2019
publisher Bio-Protocol
record_format MEDLINE/PubMed
spelling pubmed-78540982021-03-01 Production and Purification of Dengue Virus-like Particles from COS-1 Cells Galula, Jedhan Ucat Chang, Gwong-Jen J. Chao, Day-Yu Bio Protoc Methods Article Non-infectious virus-like particles (VLPs) containing dengue virus (DENV) pre-membrane (prM) and envelope (E) proteins have been demonstrated to be highly immunogenic and can be used as a potential vaccine candidate as well as a tool for serodiagnostic assays. Successful application of VLPs requires abundant, and high-purity production methods. Here, we describe a robust protocol for producing DENV VLPs from transiently-transformed or stable COS-1 cells and further provide an easily adaptable antigen purification method by sucrose gradient centrifugation. Bio-Protocol 2019-06-20 /pmc/articles/PMC7854098/ /pubmed/33654796 http://dx.doi.org/10.21769/BioProtoc.3280 Text en ©Copyright Galula et al. http://creativecommons.org/licenses/by/4.0/ This article is distributed under the terms of the Creative Commons Attribution License (CC BY 4.0).
spellingShingle Methods Article
Galula, Jedhan Ucat
Chang, Gwong-Jen J.
Chao, Day-Yu
Production and Purification of Dengue Virus-like Particles from COS-1 Cells
title Production and Purification of Dengue Virus-like Particles from COS-1 Cells
title_full Production and Purification of Dengue Virus-like Particles from COS-1 Cells
title_fullStr Production and Purification of Dengue Virus-like Particles from COS-1 Cells
title_full_unstemmed Production and Purification of Dengue Virus-like Particles from COS-1 Cells
title_short Production and Purification of Dengue Virus-like Particles from COS-1 Cells
title_sort production and purification of dengue virus-like particles from cos-1 cells
topic Methods Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7854098/
https://www.ncbi.nlm.nih.gov/pubmed/33654796
http://dx.doi.org/10.21769/BioProtoc.3280
work_keys_str_mv AT galulajedhanucat productionandpurificationofdengueviruslikeparticlesfromcos1cells
AT changgwongjenj productionandpurificationofdengueviruslikeparticlesfromcos1cells
AT chaodayyu productionandpurificationofdengueviruslikeparticlesfromcos1cells

Ejemplares similares