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Late Male-Killing Viruses in Homona magnanima Identified as Osugoroshi Viruses, Novel Members of Partitiviridae

Late male-killing, a male-specific death after hatching, is a unique phenotype found in Homona magnanima, oriental tea tortrix. The male-killing agent was suspected to be an RNA virus, but details were unknown. We herein successfully isolated and identified the putative male-killing virus as Osugoro...

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Autores principales: Fujita, Ryosuke, Inoue, Maki N., Takamatsu, Takumi, Arai, Hiroshi, Nishino, Mayu, Abe, Nobuhiko, Itokawa, Kentaro, Nakai, Madoka, Urayama, Syun-ichi, Chiba, Yuto, Amoa-Bosompem, Michael, Kunimi, Yasuhisa
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7854922/
https://www.ncbi.nlm.nih.gov/pubmed/33552030
http://dx.doi.org/10.3389/fmicb.2020.620623
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author Fujita, Ryosuke
Inoue, Maki N.
Takamatsu, Takumi
Arai, Hiroshi
Nishino, Mayu
Abe, Nobuhiko
Itokawa, Kentaro
Nakai, Madoka
Urayama, Syun-ichi
Chiba, Yuto
Amoa-Bosompem, Michael
Kunimi, Yasuhisa
author_facet Fujita, Ryosuke
Inoue, Maki N.
Takamatsu, Takumi
Arai, Hiroshi
Nishino, Mayu
Abe, Nobuhiko
Itokawa, Kentaro
Nakai, Madoka
Urayama, Syun-ichi
Chiba, Yuto
Amoa-Bosompem, Michael
Kunimi, Yasuhisa
author_sort Fujita, Ryosuke
collection PubMed
description Late male-killing, a male-specific death after hatching, is a unique phenotype found in Homona magnanima, oriental tea tortrix. The male-killing agent was suspected to be an RNA virus, but details were unknown. We herein successfully isolated and identified the putative male-killing virus as Osugoroshi viruses (OGVs). The three RNA-dependent RNA polymerase genes detected were phylogenetically related to Partitiviridae, a group of segmented double-stranded RNA viruses. Purified dsRNA from a late male-killing strain of H. magnanima revealed 24 segments, in addition to the RdRps, with consensus terminal sequences. These segments included the previously found male-killing agents MK1068 (herein OGV-related RNA16) and MK1241 (OGV-related RNA7) RNAs. Ultramicroscopic observation of purified virions, which induced late male-killing in the progeny of injected moths, showed sizes typical of Partitiviridae. Mathematical modeling showed the importance of late male-killing in facilitating horizontal transmission of OGVs in an H. magnanima population. This study is the first report on the isolation of partiti-like virus from insects, and one thought to be associated with late male-killing, although the viral genomic contents and combinations in each virus are still unknown.
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spelling pubmed-78549222021-02-04 Late Male-Killing Viruses in Homona magnanima Identified as Osugoroshi Viruses, Novel Members of Partitiviridae Fujita, Ryosuke Inoue, Maki N. Takamatsu, Takumi Arai, Hiroshi Nishino, Mayu Abe, Nobuhiko Itokawa, Kentaro Nakai, Madoka Urayama, Syun-ichi Chiba, Yuto Amoa-Bosompem, Michael Kunimi, Yasuhisa Front Microbiol Microbiology Late male-killing, a male-specific death after hatching, is a unique phenotype found in Homona magnanima, oriental tea tortrix. The male-killing agent was suspected to be an RNA virus, but details were unknown. We herein successfully isolated and identified the putative male-killing virus as Osugoroshi viruses (OGVs). The three RNA-dependent RNA polymerase genes detected were phylogenetically related to Partitiviridae, a group of segmented double-stranded RNA viruses. Purified dsRNA from a late male-killing strain of H. magnanima revealed 24 segments, in addition to the RdRps, with consensus terminal sequences. These segments included the previously found male-killing agents MK1068 (herein OGV-related RNA16) and MK1241 (OGV-related RNA7) RNAs. Ultramicroscopic observation of purified virions, which induced late male-killing in the progeny of injected moths, showed sizes typical of Partitiviridae. Mathematical modeling showed the importance of late male-killing in facilitating horizontal transmission of OGVs in an H. magnanima population. This study is the first report on the isolation of partiti-like virus from insects, and one thought to be associated with late male-killing, although the viral genomic contents and combinations in each virus are still unknown. Frontiers Media S.A. 2021-01-20 /pmc/articles/PMC7854922/ /pubmed/33552030 http://dx.doi.org/10.3389/fmicb.2020.620623 Text en Copyright © 2021 Fujita, Inoue, Takamatsu, Arai, Nishino, Abe, Itokawa, Nakai, Urayama, Chiba, Amoa-Bosompem and Kunimi. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Fujita, Ryosuke
Inoue, Maki N.
Takamatsu, Takumi
Arai, Hiroshi
Nishino, Mayu
Abe, Nobuhiko
Itokawa, Kentaro
Nakai, Madoka
Urayama, Syun-ichi
Chiba, Yuto
Amoa-Bosompem, Michael
Kunimi, Yasuhisa
Late Male-Killing Viruses in Homona magnanima Identified as Osugoroshi Viruses, Novel Members of Partitiviridae
title Late Male-Killing Viruses in Homona magnanima Identified as Osugoroshi Viruses, Novel Members of Partitiviridae
title_full Late Male-Killing Viruses in Homona magnanima Identified as Osugoroshi Viruses, Novel Members of Partitiviridae
title_fullStr Late Male-Killing Viruses in Homona magnanima Identified as Osugoroshi Viruses, Novel Members of Partitiviridae
title_full_unstemmed Late Male-Killing Viruses in Homona magnanima Identified as Osugoroshi Viruses, Novel Members of Partitiviridae
title_short Late Male-Killing Viruses in Homona magnanima Identified as Osugoroshi Viruses, Novel Members of Partitiviridae
title_sort late male-killing viruses in homona magnanima identified as osugoroshi viruses, novel members of partitiviridae
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7854922/
https://www.ncbi.nlm.nih.gov/pubmed/33552030
http://dx.doi.org/10.3389/fmicb.2020.620623
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