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Proximal and Distal Parts of Sweetpotato Adventitious Roots Display Differences in Root Architecture, Lignin, and Starch Metabolism and Their Developmental Fates

Sweetpotato is an important food crop globally, serving as a rich source of carbohydrates, vitamins, fiber, and micronutrients. Sweetpotato yield depends on the modification of adventitious roots into storage roots. The underlying mechanism of this developmental switch is not fully understood. Inter...

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Autores principales: Singh, Vikram, Zemach, Hanita, Shabtai, Sara, Aloni, Roni, Yang, Jun, Zhang, Peng, Sergeeva, Lidiya, Ligterink, Wilco, Firon, Nurit
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7855870/
https://www.ncbi.nlm.nih.gov/pubmed/33552103
http://dx.doi.org/10.3389/fpls.2020.609923
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author Singh, Vikram
Zemach, Hanita
Shabtai, Sara
Aloni, Roni
Yang, Jun
Zhang, Peng
Sergeeva, Lidiya
Ligterink, Wilco
Firon, Nurit
author_facet Singh, Vikram
Zemach, Hanita
Shabtai, Sara
Aloni, Roni
Yang, Jun
Zhang, Peng
Sergeeva, Lidiya
Ligterink, Wilco
Firon, Nurit
author_sort Singh, Vikram
collection PubMed
description Sweetpotato is an important food crop globally, serving as a rich source of carbohydrates, vitamins, fiber, and micronutrients. Sweetpotato yield depends on the modification of adventitious roots into storage roots. The underlying mechanism of this developmental switch is not fully understood. Interestingly, storage-root formation is manifested by formation of starch-accumulating parenchyma cells and bulking of the distal part of the root, while the proximal part does not show bulking. This system, where two parts of the same adventitious root display different developmental fates, was used by us in order to better characterize the anatomical, physiological, and molecular mechanisms involved in sweetpotato storage-root formation. We show that, as early as 1 and 2 weeks after planting, the proximal part of the root exhibited enhanced xylem development together with increased/massive lignin deposition, while, at the same time, the distal root part exhibited significantly elevated starch accumulation. In accordance with these developmental differences, the proximal root part exhibited up-regulated transcript levels of sweetpotato orthologs of Arabidopsis vascular-development regulators and key genes of lignin biosynthesis, while the distal part showed up-regulation of genes encoding enzymes of starch biosynthesis. All these recorded differences between proximal and distal root parts were further enhanced at 5 weeks after planting, when storage roots were formed at the distal part. Our results point to down-regulation of fiber formation and lignification, together with up-regulation of starch biosynthesis, as the main events underlying storage-root formation, marking/highlighting several genes as potential regulators, providing a valuable database of genes for further research.
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spelling pubmed-78558702021-02-04 Proximal and Distal Parts of Sweetpotato Adventitious Roots Display Differences in Root Architecture, Lignin, and Starch Metabolism and Their Developmental Fates Singh, Vikram Zemach, Hanita Shabtai, Sara Aloni, Roni Yang, Jun Zhang, Peng Sergeeva, Lidiya Ligterink, Wilco Firon, Nurit Front Plant Sci Plant Science Sweetpotato is an important food crop globally, serving as a rich source of carbohydrates, vitamins, fiber, and micronutrients. Sweetpotato yield depends on the modification of adventitious roots into storage roots. The underlying mechanism of this developmental switch is not fully understood. Interestingly, storage-root formation is manifested by formation of starch-accumulating parenchyma cells and bulking of the distal part of the root, while the proximal part does not show bulking. This system, where two parts of the same adventitious root display different developmental fates, was used by us in order to better characterize the anatomical, physiological, and molecular mechanisms involved in sweetpotato storage-root formation. We show that, as early as 1 and 2 weeks after planting, the proximal part of the root exhibited enhanced xylem development together with increased/massive lignin deposition, while, at the same time, the distal root part exhibited significantly elevated starch accumulation. In accordance with these developmental differences, the proximal root part exhibited up-regulated transcript levels of sweetpotato orthologs of Arabidopsis vascular-development regulators and key genes of lignin biosynthesis, while the distal part showed up-regulation of genes encoding enzymes of starch biosynthesis. All these recorded differences between proximal and distal root parts were further enhanced at 5 weeks after planting, when storage roots were formed at the distal part. Our results point to down-regulation of fiber formation and lignification, together with up-regulation of starch biosynthesis, as the main events underlying storage-root formation, marking/highlighting several genes as potential regulators, providing a valuable database of genes for further research. Frontiers Media S.A. 2021-01-21 /pmc/articles/PMC7855870/ /pubmed/33552103 http://dx.doi.org/10.3389/fpls.2020.609923 Text en Copyright © 2021 Singh, Zemach, Shabtai, Aloni, Yang, Zhang, Sergeeva, Ligterink and Firon. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Plant Science
Singh, Vikram
Zemach, Hanita
Shabtai, Sara
Aloni, Roni
Yang, Jun
Zhang, Peng
Sergeeva, Lidiya
Ligterink, Wilco
Firon, Nurit
Proximal and Distal Parts of Sweetpotato Adventitious Roots Display Differences in Root Architecture, Lignin, and Starch Metabolism and Their Developmental Fates
title Proximal and Distal Parts of Sweetpotato Adventitious Roots Display Differences in Root Architecture, Lignin, and Starch Metabolism and Their Developmental Fates
title_full Proximal and Distal Parts of Sweetpotato Adventitious Roots Display Differences in Root Architecture, Lignin, and Starch Metabolism and Their Developmental Fates
title_fullStr Proximal and Distal Parts of Sweetpotato Adventitious Roots Display Differences in Root Architecture, Lignin, and Starch Metabolism and Their Developmental Fates
title_full_unstemmed Proximal and Distal Parts of Sweetpotato Adventitious Roots Display Differences in Root Architecture, Lignin, and Starch Metabolism and Their Developmental Fates
title_short Proximal and Distal Parts of Sweetpotato Adventitious Roots Display Differences in Root Architecture, Lignin, and Starch Metabolism and Their Developmental Fates
title_sort proximal and distal parts of sweetpotato adventitious roots display differences in root architecture, lignin, and starch metabolism and their developmental fates
topic Plant Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7855870/
https://www.ncbi.nlm.nih.gov/pubmed/33552103
http://dx.doi.org/10.3389/fpls.2020.609923
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