In vivo regeneration of neo-nephrons in rodents by renal progenitor cell transplantation

Renal progenitor cells induced from pluripotent stem cells have attracted attention as a cell source for organ regeneration. Here, we report an in vivo protocol for the regeneration of urine-producing nephrons, i.e., neo-nephrons, in mice. We outline steps to transplant exogenous renal progenitor ce...

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Detalles Bibliográficos
Autores principales: Yamanaka, Shuichiro, Matsui, Kenji, Fujimoto, Toshinari, Takamura, Tsuyoshi, Saito, Yatsumu, Matsumoto, Naoto, Tajiri, Susumu, Matsumoto, Kei, Yokoo, Takashi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7856474/
https://www.ncbi.nlm.nih.gov/pubmed/33554147
http://dx.doi.org/10.1016/j.xpro.2021.100314
Descripción
Sumario:Renal progenitor cells induced from pluripotent stem cells have attracted attention as a cell source for organ regeneration. Here, we report an in vivo protocol for the regeneration of urine-producing nephrons, i.e., neo-nephrons, in mice. We outline steps to transplant exogenous renal progenitor cells into the nephrogenic zone of transgenic mice and subsequently analyze these neo-nephrons. For complete details on the use and execution of this protocol, please refer to Fujimoto et al. (2020).

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