In vivo regeneration of neo-nephrons in rodents by renal progenitor cell transplantation

Renal progenitor cells induced from pluripotent stem cells have attracted attention as a cell source for organ regeneration. Here, we report an in vivo protocol for the regeneration of urine-producing nephrons, i.e., neo-nephrons, in mice. We outline steps to transplant exogenous renal progenitor ce...

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Autores principales: Yamanaka, Shuichiro, Matsui, Kenji, Fujimoto, Toshinari, Takamura, Tsuyoshi, Saito, Yatsumu, Matsumoto, Naoto, Tajiri, Susumu, Matsumoto, Kei, Yokoo, Takashi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7856474/
https://www.ncbi.nlm.nih.gov/pubmed/33554147
http://dx.doi.org/10.1016/j.xpro.2021.100314
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author Yamanaka, Shuichiro
Matsui, Kenji
Fujimoto, Toshinari
Takamura, Tsuyoshi
Saito, Yatsumu
Matsumoto, Naoto
Tajiri, Susumu
Matsumoto, Kei
Yokoo, Takashi
author_facet Yamanaka, Shuichiro
Matsui, Kenji
Fujimoto, Toshinari
Takamura, Tsuyoshi
Saito, Yatsumu
Matsumoto, Naoto
Tajiri, Susumu
Matsumoto, Kei
Yokoo, Takashi
author_sort Yamanaka, Shuichiro
collection PubMed
description Renal progenitor cells induced from pluripotent stem cells have attracted attention as a cell source for organ regeneration. Here, we report an in vivo protocol for the regeneration of urine-producing nephrons, i.e., neo-nephrons, in mice. We outline steps to transplant exogenous renal progenitor cells into the nephrogenic zone of transgenic mice and subsequently analyze these neo-nephrons. For complete details on the use and execution of this protocol, please refer to Fujimoto et al. (2020).
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spelling pubmed-78564742021-02-05 In vivo regeneration of neo-nephrons in rodents by renal progenitor cell transplantation Yamanaka, Shuichiro Matsui, Kenji Fujimoto, Toshinari Takamura, Tsuyoshi Saito, Yatsumu Matsumoto, Naoto Tajiri, Susumu Matsumoto, Kei Yokoo, Takashi STAR Protoc Protocol Renal progenitor cells induced from pluripotent stem cells have attracted attention as a cell source for organ regeneration. Here, we report an in vivo protocol for the regeneration of urine-producing nephrons, i.e., neo-nephrons, in mice. We outline steps to transplant exogenous renal progenitor cells into the nephrogenic zone of transgenic mice and subsequently analyze these neo-nephrons. For complete details on the use and execution of this protocol, please refer to Fujimoto et al. (2020). Elsevier 2021-02-01 /pmc/articles/PMC7856474/ /pubmed/33554147 http://dx.doi.org/10.1016/j.xpro.2021.100314 Text en © 2021 The Author(s) http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Protocol
Yamanaka, Shuichiro
Matsui, Kenji
Fujimoto, Toshinari
Takamura, Tsuyoshi
Saito, Yatsumu
Matsumoto, Naoto
Tajiri, Susumu
Matsumoto, Kei
Yokoo, Takashi
In vivo regeneration of neo-nephrons in rodents by renal progenitor cell transplantation
title In vivo regeneration of neo-nephrons in rodents by renal progenitor cell transplantation
title_full In vivo regeneration of neo-nephrons in rodents by renal progenitor cell transplantation
title_fullStr In vivo regeneration of neo-nephrons in rodents by renal progenitor cell transplantation
title_full_unstemmed In vivo regeneration of neo-nephrons in rodents by renal progenitor cell transplantation
title_short In vivo regeneration of neo-nephrons in rodents by renal progenitor cell transplantation
title_sort in vivo regeneration of neo-nephrons in rodents by renal progenitor cell transplantation
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7856474/
https://www.ncbi.nlm.nih.gov/pubmed/33554147
http://dx.doi.org/10.1016/j.xpro.2021.100314
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