Cargando…

Effects of rutin on osteoblast MC3T3-E1 differentiation, ALP activity and Runx2 protein expression

As a flavonoid, rutin has been found to have a wide range of biological functions, such as resisting inflammation and oxidation, and preventing cerebral hemorrhage and hypertension. It has been found to play an important role in osteoporosis and other orthopedic diseases in recent years. MC3T3-E1 ce...

Descripción completa

Detalles Bibliográficos
Autores principales: Liu, Xin-Wei, Ma, Bin, Zi, Ying, Xiang, Liang-Bi, Han, Tian-Yu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: PAGEPress Publications, Pavia, Italy 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7856824/
https://www.ncbi.nlm.nih.gov/pubmed/33478200
http://dx.doi.org/10.4081/ejh.2021.3195
_version_ 1783646321803001856
author Liu, Xin-Wei
Ma, Bin
Zi, Ying
Xiang, Liang-Bi
Han, Tian-Yu
author_facet Liu, Xin-Wei
Ma, Bin
Zi, Ying
Xiang, Liang-Bi
Han, Tian-Yu
author_sort Liu, Xin-Wei
collection PubMed
description As a flavonoid, rutin has been found to have a wide range of biological functions, such as resisting inflammation and oxidation, and preventing cerebral hemorrhage and hypertension. It has been found to play an important role in osteoporosis and other orthopedic diseases in recent years. MC3T3-E1 cells were randomly divided into a control group, a rutin-1 group (0.01 mmol/L), a rutin-2 group (0.05 mmol/L) and a rutin-3 group (0.1 mmol/L). Osteogenic differentiation of cells was induced by osteogenic induction fluid. The control group was treated with the maximum dose of drug solvent. 2~3 days later, the solvent was replaced with fresh osteogenic induction fluid containing rutin. After a certain period of routine culture, the cells were collected for subsequent experiments. The expression of Runx2 gene in cells in all groups was detected by Real-time PCR; the expression of Runx2 protein was detected by Western blot and immunocytochemistry (IHC); the activity of ALP was detected by reagent kit method; osteogenic differentiation was analyzed by alizarin red staining. The results of Real-time PCR showed that, compared with the control group, the treatment of cells with rutin can significantly increase the expression of Runx2 gene (p<0.05); the higher the concentration, the higher the expression of Runx2 gene, and significant differences were found among groups in which different concentrations were used (p<0.05); the results of Western blot and IHC showed that the expression trend of Runx2 protein in each group was consistent with PCR results. In drug treatment groups, the activity of ALP was significantly higher than that in the control group (p<0.05); there were significant differences among groups in which different concentrations were used (p<0.05). The results of alizarin red staining showed that calcified nodules were formed in all groups and that the area of calcified nodules formed in groups treated with rutin was greater than that in the control group; the greater the concentration, the larger the area. Rutin can promote osteoblastic differentiation; and the greater the concentration, the more effective it is.
format Online
Article
Text
id pubmed-7856824
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher PAGEPress Publications, Pavia, Italy
record_format MEDLINE/PubMed
spelling pubmed-78568242021-02-04 Effects of rutin on osteoblast MC3T3-E1 differentiation, ALP activity and Runx2 protein expression Liu, Xin-Wei Ma, Bin Zi, Ying Xiang, Liang-Bi Han, Tian-Yu Eur J Histochem Article As a flavonoid, rutin has been found to have a wide range of biological functions, such as resisting inflammation and oxidation, and preventing cerebral hemorrhage and hypertension. It has been found to play an important role in osteoporosis and other orthopedic diseases in recent years. MC3T3-E1 cells were randomly divided into a control group, a rutin-1 group (0.01 mmol/L), a rutin-2 group (0.05 mmol/L) and a rutin-3 group (0.1 mmol/L). Osteogenic differentiation of cells was induced by osteogenic induction fluid. The control group was treated with the maximum dose of drug solvent. 2~3 days later, the solvent was replaced with fresh osteogenic induction fluid containing rutin. After a certain period of routine culture, the cells were collected for subsequent experiments. The expression of Runx2 gene in cells in all groups was detected by Real-time PCR; the expression of Runx2 protein was detected by Western blot and immunocytochemistry (IHC); the activity of ALP was detected by reagent kit method; osteogenic differentiation was analyzed by alizarin red staining. The results of Real-time PCR showed that, compared with the control group, the treatment of cells with rutin can significantly increase the expression of Runx2 gene (p<0.05); the higher the concentration, the higher the expression of Runx2 gene, and significant differences were found among groups in which different concentrations were used (p<0.05); the results of Western blot and IHC showed that the expression trend of Runx2 protein in each group was consistent with PCR results. In drug treatment groups, the activity of ALP was significantly higher than that in the control group (p<0.05); there were significant differences among groups in which different concentrations were used (p<0.05). The results of alizarin red staining showed that calcified nodules were formed in all groups and that the area of calcified nodules formed in groups treated with rutin was greater than that in the control group; the greater the concentration, the larger the area. Rutin can promote osteoblastic differentiation; and the greater the concentration, the more effective it is. PAGEPress Publications, Pavia, Italy 2021-01-20 /pmc/articles/PMC7856824/ /pubmed/33478200 http://dx.doi.org/10.4081/ejh.2021.3195 Text en ©Copyright: the Author(s) http://creativecommons.org/licenses/by-nc/4.0/ This work is licensed under a Creative Commons Attribution NonCommercial 4.0 License (CC BY-NC 4.0).
spellingShingle Article
Liu, Xin-Wei
Ma, Bin
Zi, Ying
Xiang, Liang-Bi
Han, Tian-Yu
Effects of rutin on osteoblast MC3T3-E1 differentiation, ALP activity and Runx2 protein expression
title Effects of rutin on osteoblast MC3T3-E1 differentiation, ALP activity and Runx2 protein expression
title_full Effects of rutin on osteoblast MC3T3-E1 differentiation, ALP activity and Runx2 protein expression
title_fullStr Effects of rutin on osteoblast MC3T3-E1 differentiation, ALP activity and Runx2 protein expression
title_full_unstemmed Effects of rutin on osteoblast MC3T3-E1 differentiation, ALP activity and Runx2 protein expression
title_short Effects of rutin on osteoblast MC3T3-E1 differentiation, ALP activity and Runx2 protein expression
title_sort effects of rutin on osteoblast mc3t3-e1 differentiation, alp activity and runx2 protein expression
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7856824/
https://www.ncbi.nlm.nih.gov/pubmed/33478200
http://dx.doi.org/10.4081/ejh.2021.3195
work_keys_str_mv AT liuxinwei effectsofrutinonosteoblastmc3t3e1differentiationalpactivityandrunx2proteinexpression
AT mabin effectsofrutinonosteoblastmc3t3e1differentiationalpactivityandrunx2proteinexpression
AT ziying effectsofrutinonosteoblastmc3t3e1differentiationalpactivityandrunx2proteinexpression
AT xiangliangbi effectsofrutinonosteoblastmc3t3e1differentiationalpactivityandrunx2proteinexpression
AT hantianyu effectsofrutinonosteoblastmc3t3e1differentiationalpactivityandrunx2proteinexpression