Preexisting and de novo humoral immunity to SARS-CoV-2 in humans

Zoonotic introduction of novel coronaviruses may encounter preexisting immunity in humans. Using diverse assays for antibodies recognizing SARS-CoV-2 proteins, we detected preexisting humoral immunity. SARS-CoV-2 spike glycoprotein (S)–reactive antibodies were detectable using a flow cytometry–based...

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Detalles Bibliográficos
Autores principales: Ng, Kevin W., Faulkner, Nikhil, Cornish, Georgina H., Rosa, Annachiara, Harvey, Ruth, Hussain, Saira, Ulferts, Rachel, Earl, Christopher, Wrobel, Antoni G., Benton, Donald J., Roustan, Chloe, Bolland, William, Thompson, Rachael, Agua-Doce, Ana, Hobson, Philip, Heaney, Judith, Rickman, Hannah, Paraskevopoulou, Stavroula, Houlihan, Catherine F., Thomson, Kirsty, Sanchez, Emilie, Shin, Gee Yen, Spyer, Moira J., Joshi, Dhira, O’Reilly, Nicola, Walker, Philip A., Kjaer, Svend, Riddell, Andrew, Moore, Catherine, Jebson, Bethany R., Wilkinson, Meredyth, Marshall, Lucy R., Rosser, Elizabeth C., Radziszewska, Anna, Peckham, Hannah, Ciurtin, Coziana, Wedderburn, Lucy R., Beale, Rupert, Swanton, Charles, Gandhi, Sonia, Stockinger, Brigitta, McCauley, John, Gamblin, Steve J., McCoy, Laura E., Cherepanov, Peter, Nastouli, Eleni, Kassiotis, George
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Association for the Advancement of Science 2020
Materias:
Reports
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7857411/
https://www.ncbi.nlm.nih.gov/pubmed/33159009
http://dx.doi.org/10.1126/science.abe1107
Descripción
Sumario:Zoonotic introduction of novel coronaviruses may encounter preexisting immunity in humans. Using diverse assays for antibodies recognizing SARS-CoV-2 proteins, we detected preexisting humoral immunity. SARS-CoV-2 spike glycoprotein (S)–reactive antibodies were detectable using a flow cytometry–based method in SARS-CoV-2–uninfected individuals and were particularly prevalent in children and adolescents. They were predominantly of the immunoglobulin G (IgG) class and targeted the S2 subunit. By contrast, SARS-CoV-2 infection induced higher titers of SARS-CoV-2 S–reactive IgG antibodies targeting both the S1 and S2 subunits, and concomitant IgM and IgA antibodies, lasting throughout the observation period. SARS-CoV-2–uninfected donor sera exhibited specific neutralizing activity against SARS-CoV-2 and SARS-CoV-2 S pseudotypes. Distinguishing preexisting and de novo immunity will be critical for our understanding of susceptibility to and the natural course of SARS-CoV-2 infection.

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