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Chinese Traditional Medicine NiuBeiXiaoHe (NBXH) Extracts Have the Function of Antituberculosis and Immune Recovery in BALB/c Mice

BACKGROUND: The Traditional Chinese Medicine NiuBeiXiaoHe (NBXH) is a valid antituberculosis (TB) prescription from the experience of clinical practice. However, the mechanism of NBXH extracts' immunotherapy has been poorly understood. Herein, the immunotherapeutic efficacy and the differential...

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Autores principales: Liang, Yan, Gong, Wenping, Wang, Xiaomei, Zhang, Junxian, Ling, Yanbo, Song, Jinying, Wang, Lan, Liu, Xiao, Wang, Jie, Yang, Yourong, Chen, Shibing, Liu, Jun, Yang, Chunwei, Luo, Huafeng, Wu, Xueqiong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7857905/
https://www.ncbi.nlm.nih.gov/pubmed/33575361
http://dx.doi.org/10.1155/2021/6234560
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author Liang, Yan
Gong, Wenping
Wang, Xiaomei
Zhang, Junxian
Ling, Yanbo
Song, Jinying
Wang, Lan
Liu, Xiao
Wang, Jie
Yang, Yourong
Chen, Shibing
Liu, Jun
Yang, Chunwei
Luo, Huafeng
Wu, Xueqiong
author_facet Liang, Yan
Gong, Wenping
Wang, Xiaomei
Zhang, Junxian
Ling, Yanbo
Song, Jinying
Wang, Lan
Liu, Xiao
Wang, Jie
Yang, Yourong
Chen, Shibing
Liu, Jun
Yang, Chunwei
Luo, Huafeng
Wu, Xueqiong
author_sort Liang, Yan
collection PubMed
description BACKGROUND: The Traditional Chinese Medicine NiuBeiXiaoHe (NBXH) is a valid antituberculosis (TB) prescription from the experience of clinical practice. However, the mechanism of NBXH extracts' immunotherapy has been poorly understood. Herein, the immunotherapeutic efficacy and the differentially expressed (DE) genes of NBXH extracts were evaluated and identified in BALB/c mice. METHODS: The total RNA was extracted from peripheral blood mononuclear cells, and the DE genes were identified by gene chip. The enrichment and signaling pathway analyses were performed using Gene Ontology (GO) and KEGG database. RESULTS: It was shown that the treatment of NBXH extracts (high dose) significantly reduced mycobacteria loads and histopathological lesions in mice infected by Mycobacterium tuberculosis and resulted in 3,454 DE upregulated genes and 3,594 downregulated DE genes. Furthermore, NBXH extracts killed mycobacteria by inhibiting the supply of necessary ingredients for their growth and proliferation. They restored the disordered immune microenvironments by up- or downregulating immune and inflammation-related pathways. CONCLUSIONS: Taken together, NBXH extracts not only efficiently decreased the mycobacteria loads but also balanced the immune disorders in mice. These new findings provide a fresh perspective for elucidating the immunotherapeutic mechanism of NBXH extracts and pointed out the direction for improving the treatment efficacy of NBXH extracts.
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spelling pubmed-78579052021-02-10 Chinese Traditional Medicine NiuBeiXiaoHe (NBXH) Extracts Have the Function of Antituberculosis and Immune Recovery in BALB/c Mice Liang, Yan Gong, Wenping Wang, Xiaomei Zhang, Junxian Ling, Yanbo Song, Jinying Wang, Lan Liu, Xiao Wang, Jie Yang, Yourong Chen, Shibing Liu, Jun Yang, Chunwei Luo, Huafeng Wu, Xueqiong J Immunol Res Research Article BACKGROUND: The Traditional Chinese Medicine NiuBeiXiaoHe (NBXH) is a valid antituberculosis (TB) prescription from the experience of clinical practice. However, the mechanism of NBXH extracts' immunotherapy has been poorly understood. Herein, the immunotherapeutic efficacy and the differentially expressed (DE) genes of NBXH extracts were evaluated and identified in BALB/c mice. METHODS: The total RNA was extracted from peripheral blood mononuclear cells, and the DE genes were identified by gene chip. The enrichment and signaling pathway analyses were performed using Gene Ontology (GO) and KEGG database. RESULTS: It was shown that the treatment of NBXH extracts (high dose) significantly reduced mycobacteria loads and histopathological lesions in mice infected by Mycobacterium tuberculosis and resulted in 3,454 DE upregulated genes and 3,594 downregulated DE genes. Furthermore, NBXH extracts killed mycobacteria by inhibiting the supply of necessary ingredients for their growth and proliferation. They restored the disordered immune microenvironments by up- or downregulating immune and inflammation-related pathways. CONCLUSIONS: Taken together, NBXH extracts not only efficiently decreased the mycobacteria loads but also balanced the immune disorders in mice. These new findings provide a fresh perspective for elucidating the immunotherapeutic mechanism of NBXH extracts and pointed out the direction for improving the treatment efficacy of NBXH extracts. Hindawi 2021-01-18 /pmc/articles/PMC7857905/ /pubmed/33575361 http://dx.doi.org/10.1155/2021/6234560 Text en Copyright © 2021 Yan Liang et al. https://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Liang, Yan
Gong, Wenping
Wang, Xiaomei
Zhang, Junxian
Ling, Yanbo
Song, Jinying
Wang, Lan
Liu, Xiao
Wang, Jie
Yang, Yourong
Chen, Shibing
Liu, Jun
Yang, Chunwei
Luo, Huafeng
Wu, Xueqiong
Chinese Traditional Medicine NiuBeiXiaoHe (NBXH) Extracts Have the Function of Antituberculosis and Immune Recovery in BALB/c Mice
title Chinese Traditional Medicine NiuBeiXiaoHe (NBXH) Extracts Have the Function of Antituberculosis and Immune Recovery in BALB/c Mice
title_full Chinese Traditional Medicine NiuBeiXiaoHe (NBXH) Extracts Have the Function of Antituberculosis and Immune Recovery in BALB/c Mice
title_fullStr Chinese Traditional Medicine NiuBeiXiaoHe (NBXH) Extracts Have the Function of Antituberculosis and Immune Recovery in BALB/c Mice
title_full_unstemmed Chinese Traditional Medicine NiuBeiXiaoHe (NBXH) Extracts Have the Function of Antituberculosis and Immune Recovery in BALB/c Mice
title_short Chinese Traditional Medicine NiuBeiXiaoHe (NBXH) Extracts Have the Function of Antituberculosis and Immune Recovery in BALB/c Mice
title_sort chinese traditional medicine niubeixiaohe (nbxh) extracts have the function of antituberculosis and immune recovery in balb/c mice
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7857905/
https://www.ncbi.nlm.nih.gov/pubmed/33575361
http://dx.doi.org/10.1155/2021/6234560
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