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Percentage of Myeloid Dendritic Cells in Peripheral Venous Blood Is Negatively Related to Incidence of Graves' Orbitopathy
The aim of the study was to evaluate the distribution of blood dendritic cells (DCs) in patients with Graves' orbitopathy (GO) and to assess the influence of methylprednisolone therapy on subsets of peripheral blood mononuclear cells (PBMCs). Peripheral blood DC subsets were analyzed by flow cy...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Hindawi
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7857924/ https://www.ncbi.nlm.nih.gov/pubmed/33574732 http://dx.doi.org/10.1155/2021/8896055 |
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author | Wojciechowska-Durczynska, Katarzyna Wieczorek-Szukala, Katarzyna Stefanski, Borys Zygmunt, Arkadiusz Stepniak, Jan Karbownik-Lewinska, Małgorzata Lewinski, Andrzej |
author_facet | Wojciechowska-Durczynska, Katarzyna Wieczorek-Szukala, Katarzyna Stefanski, Borys Zygmunt, Arkadiusz Stepniak, Jan Karbownik-Lewinska, Małgorzata Lewinski, Andrzej |
author_sort | Wojciechowska-Durczynska, Katarzyna |
collection | PubMed |
description | The aim of the study was to evaluate the distribution of blood dendritic cells (DCs) in patients with Graves' orbitopathy (GO) and to assess the influence of methylprednisolone therapy on subsets of peripheral blood mononuclear cells (PBMCs). Peripheral blood DC subsets were analyzed by flow cytometry in patients with active GO (n = 17), inactive GO (n = 8), and Graves' disease (GD) without GO (n = 8) and controls (n = 15); additionally, in patients with active GO (n = 17), analyses were done at three time points, i.e., before methylprednisolone treatment and after 6 weeks and after 12 weeks of the treatment. Percentage of myeloid DCs (mDCs) in PBMC fraction was significantly lower in patients with both active and inactive GO, compared to patients with GD without GO and controls (p < 0.05). In addition, mDCs were also documented to be an independent factor negatively associated with GO, however without essential differences between active and inactive phases. On the other hand, we did not observe any changes in the percentage of DCs after methylprednisolone therapy (p > 0.05). In the present study, we have succeeded to firstly demonstrate—according to our knowledge—that blood mDCs are negatively related to GO incidence. |
format | Online Article Text |
id | pubmed-7857924 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Hindawi |
record_format | MEDLINE/PubMed |
spelling | pubmed-78579242021-02-10 Percentage of Myeloid Dendritic Cells in Peripheral Venous Blood Is Negatively Related to Incidence of Graves' Orbitopathy Wojciechowska-Durczynska, Katarzyna Wieczorek-Szukala, Katarzyna Stefanski, Borys Zygmunt, Arkadiusz Stepniak, Jan Karbownik-Lewinska, Małgorzata Lewinski, Andrzej Mediators Inflamm Research Article The aim of the study was to evaluate the distribution of blood dendritic cells (DCs) in patients with Graves' orbitopathy (GO) and to assess the influence of methylprednisolone therapy on subsets of peripheral blood mononuclear cells (PBMCs). Peripheral blood DC subsets were analyzed by flow cytometry in patients with active GO (n = 17), inactive GO (n = 8), and Graves' disease (GD) without GO (n = 8) and controls (n = 15); additionally, in patients with active GO (n = 17), analyses were done at three time points, i.e., before methylprednisolone treatment and after 6 weeks and after 12 weeks of the treatment. Percentage of myeloid DCs (mDCs) in PBMC fraction was significantly lower in patients with both active and inactive GO, compared to patients with GD without GO and controls (p < 0.05). In addition, mDCs were also documented to be an independent factor negatively associated with GO, however without essential differences between active and inactive phases. On the other hand, we did not observe any changes in the percentage of DCs after methylprednisolone therapy (p > 0.05). In the present study, we have succeeded to firstly demonstrate—according to our knowledge—that blood mDCs are negatively related to GO incidence. Hindawi 2021-01-26 /pmc/articles/PMC7857924/ /pubmed/33574732 http://dx.doi.org/10.1155/2021/8896055 Text en Copyright © 2021 Katarzyna Wojciechowska-Durczynska et al. https://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Wojciechowska-Durczynska, Katarzyna Wieczorek-Szukala, Katarzyna Stefanski, Borys Zygmunt, Arkadiusz Stepniak, Jan Karbownik-Lewinska, Małgorzata Lewinski, Andrzej Percentage of Myeloid Dendritic Cells in Peripheral Venous Blood Is Negatively Related to Incidence of Graves' Orbitopathy |
title | Percentage of Myeloid Dendritic Cells in Peripheral Venous Blood Is Negatively Related to Incidence of Graves' Orbitopathy |
title_full | Percentage of Myeloid Dendritic Cells in Peripheral Venous Blood Is Negatively Related to Incidence of Graves' Orbitopathy |
title_fullStr | Percentage of Myeloid Dendritic Cells in Peripheral Venous Blood Is Negatively Related to Incidence of Graves' Orbitopathy |
title_full_unstemmed | Percentage of Myeloid Dendritic Cells in Peripheral Venous Blood Is Negatively Related to Incidence of Graves' Orbitopathy |
title_short | Percentage of Myeloid Dendritic Cells in Peripheral Venous Blood Is Negatively Related to Incidence of Graves' Orbitopathy |
title_sort | percentage of myeloid dendritic cells in peripheral venous blood is negatively related to incidence of graves' orbitopathy |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7857924/ https://www.ncbi.nlm.nih.gov/pubmed/33574732 http://dx.doi.org/10.1155/2021/8896055 |
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