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Third generation radioimmunoassay (RIA) for TSH receptor autoantibodies (TRAb) – one step less, similar results?

Aim  TSH-receptor (TSHR)-autoantibody (TRAb) is the serological hallmark of Graves’ disease (GD). Recently, 3 (rd) -generation radioimmunoassays (RIA) employing monoclonal TRAb such as M22 or T7 instead of TSH for the inhibition of human TRAb binding with solid-phase TSHR (coated tubes) have been in...

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Autores principales: Roggenbuck, Johannes J., Zarske, Grit, Schierack, Peter, Wunderlich, Gerd, Conrad, Karsten, Kotzerke, Joerg, Roggenbuck, Dirk, Zöphel, Klaus
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Georg Thieme Verlag KG 2021
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7857932/
https://www.ncbi.nlm.nih.gov/pubmed/33535269
http://dx.doi.org/10.1055/a-1277-5972
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author Roggenbuck, Johannes J.
Zarske, Grit
Schierack, Peter
Wunderlich, Gerd
Conrad, Karsten
Kotzerke, Joerg
Roggenbuck, Dirk
Zöphel, Klaus
author_facet Roggenbuck, Johannes J.
Zarske, Grit
Schierack, Peter
Wunderlich, Gerd
Conrad, Karsten
Kotzerke, Joerg
Roggenbuck, Dirk
Zöphel, Klaus
author_sort Roggenbuck, Johannes J.
collection PubMed
description Aim  TSH-receptor (TSHR)-autoantibody (TRAb) is the serological hallmark of Graves’ disease (GD). Recently, 3 (rd) -generation radioimmunoassays (RIA) employing monoclonal TRAb such as M22 or T7 instead of TSH for the inhibition of human TRAb binding with solid-phase TSHR (coated tubes) have been introduced into laboratory routine. Methods  As current assays typically employ a consecutive incubation of patient serum and labelled monoclonal TRAb, automation of TRAb RIA is a challenge. Thus, the assay procedure using human TSHR-coated tubes and the mouse monoclonal TRAb T7 was modified by combining both steps. The novel one-step method was compared with its corresponding consecutive 3 (rd) -generation RIA by investigating 304 individuals encompassing 102 patients with active GD (GD (a) ), 43 patients with GD after successful therapy (GD (t) ), 31 with Hashimoto’s disease (HD), 28 with non-autoimmune thyroid diseases (NAITD) and 100 healthy subjects (HS). Results  With the new method, the incubation time was shortened by approximately one hour. Both 3 (rd) -generation RIAs did not reveal a significantly different assay performance by comparing areas under the curve (AUC) with receiver operating characteristics curve analysis (AUC one-step: 0.94, AUC two-step: 0.96, p > 0.05, respectively). The two-step TRAb RIA demonstrated sensitivity and specificity values of 87.5 % and 96.2 %, respectively, whereas the one-step revealed 84.6 % and 96.2 %, respectively. Conclusion  One-step 3 (rd) -generation RIA may be used for the reliable detection of TRAb. The shorter and easier assay design may improve its use and enable automation in routine nuclear medicine laboratories.
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spelling pubmed-78579322021-02-05 Third generation radioimmunoassay (RIA) for TSH receptor autoantibodies (TRAb) – one step less, similar results? Roggenbuck, Johannes J. Zarske, Grit Schierack, Peter Wunderlich, Gerd Conrad, Karsten Kotzerke, Joerg Roggenbuck, Dirk Zöphel, Klaus Nuklearmedizin Aim  TSH-receptor (TSHR)-autoantibody (TRAb) is the serological hallmark of Graves’ disease (GD). Recently, 3 (rd) -generation radioimmunoassays (RIA) employing monoclonal TRAb such as M22 or T7 instead of TSH for the inhibition of human TRAb binding with solid-phase TSHR (coated tubes) have been introduced into laboratory routine. Methods  As current assays typically employ a consecutive incubation of patient serum and labelled monoclonal TRAb, automation of TRAb RIA is a challenge. Thus, the assay procedure using human TSHR-coated tubes and the mouse monoclonal TRAb T7 was modified by combining both steps. The novel one-step method was compared with its corresponding consecutive 3 (rd) -generation RIA by investigating 304 individuals encompassing 102 patients with active GD (GD (a) ), 43 patients with GD after successful therapy (GD (t) ), 31 with Hashimoto’s disease (HD), 28 with non-autoimmune thyroid diseases (NAITD) and 100 healthy subjects (HS). Results  With the new method, the incubation time was shortened by approximately one hour. Both 3 (rd) -generation RIAs did not reveal a significantly different assay performance by comparing areas under the curve (AUC) with receiver operating characteristics curve analysis (AUC one-step: 0.94, AUC two-step: 0.96, p > 0.05, respectively). The two-step TRAb RIA demonstrated sensitivity and specificity values of 87.5 % and 96.2 %, respectively, whereas the one-step revealed 84.6 % and 96.2 %, respectively. Conclusion  One-step 3 (rd) -generation RIA may be used for the reliable detection of TRAb. The shorter and easier assay design may improve its use and enable automation in routine nuclear medicine laboratories. Georg Thieme Verlag KG 2021-02 2021-02-03 /pmc/articles/PMC7857932/ /pubmed/33535269 http://dx.doi.org/10.1055/a-1277-5972 Text en The Author(s). This is an open access article published by Thieme under the terms of the Creative Commons Attribution-NonDerivative-NonCommercial License, permitting copying and reproduction so long as the original work is given appropriate credit. Contents may not be used for commecial purposes, or adapted, remixed, transformed or built upon. (https://creativecommons.org/licenses/by-nc-nd/4.0/) https://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivatives License, which permits unrestricted reproduction and distribution, for non-commercial purposes only; and use and reproduction, but not distribution, of adapted material for non-commercial purposes only, provided the original work is properly cited.
spellingShingle Roggenbuck, Johannes J.
Zarske, Grit
Schierack, Peter
Wunderlich, Gerd
Conrad, Karsten
Kotzerke, Joerg
Roggenbuck, Dirk
Zöphel, Klaus
Third generation radioimmunoassay (RIA) for TSH receptor autoantibodies (TRAb) – one step less, similar results?
title Third generation radioimmunoassay (RIA) for TSH receptor autoantibodies (TRAb) – one step less, similar results?
title_full Third generation radioimmunoassay (RIA) for TSH receptor autoantibodies (TRAb) – one step less, similar results?
title_fullStr Third generation radioimmunoassay (RIA) for TSH receptor autoantibodies (TRAb) – one step less, similar results?
title_full_unstemmed Third generation radioimmunoassay (RIA) for TSH receptor autoantibodies (TRAb) – one step less, similar results?
title_short Third generation radioimmunoassay (RIA) for TSH receptor autoantibodies (TRAb) – one step less, similar results?
title_sort third generation radioimmunoassay (ria) for tsh receptor autoantibodies (trab) – one step less, similar results?
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7857932/
https://www.ncbi.nlm.nih.gov/pubmed/33535269
http://dx.doi.org/10.1055/a-1277-5972
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