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Persistence of Human Bocavirus 1 in Tonsillar Germinal Centers and Antibody-Dependent Enhancement of Infection

Human bocavirus 1 (HBoV1), a nonenveloped single-stranded DNA parvovirus, causes mild to life-threatening respiratory tract infections, acute otitis media, and encephalitis in young children. HBoV1 often persists in nasopharyngeal secretions for months, hampering diagnosis. It has also been shown to...

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Autores principales: Xu, Man, Perdomo, Maria Fernanda, Mattola, Salla, Pyöriä, Lari, Toppinen, Mari, Qiu, Jianming, Vihinen-Ranta, Maija, Hedman, Klaus, Nokso-Koivisto, Johanna, Aaltonen, Leena-Maija, Söderlund-Venermo, Maria
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Society for Microbiology 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7858059/
https://www.ncbi.nlm.nih.gov/pubmed/33531399
http://dx.doi.org/10.1128/mBio.03132-20
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author Xu, Man
Perdomo, Maria Fernanda
Mattola, Salla
Pyöriä, Lari
Toppinen, Mari
Qiu, Jianming
Vihinen-Ranta, Maija
Hedman, Klaus
Nokso-Koivisto, Johanna
Aaltonen, Leena-Maija
Söderlund-Venermo, Maria
author_facet Xu, Man
Perdomo, Maria Fernanda
Mattola, Salla
Pyöriä, Lari
Toppinen, Mari
Qiu, Jianming
Vihinen-Ranta, Maija
Hedman, Klaus
Nokso-Koivisto, Johanna
Aaltonen, Leena-Maija
Söderlund-Venermo, Maria
author_sort Xu, Man
collection PubMed
description Human bocavirus 1 (HBoV1), a nonenveloped single-stranded DNA parvovirus, causes mild to life-threatening respiratory tract infections, acute otitis media, and encephalitis in young children. HBoV1 often persists in nasopharyngeal secretions for months, hampering diagnosis. It has also been shown to persist in pediatric palatine and adenoid tonsils, which suggests that lymphoid organs are reservoirs for virus spread; however, the tissue site and host cells remain unknown. Our aim was to determine, in healthy nonviremic children with preexisting HBoV1 immunity, the adenotonsillar persistence site(s), host cell types, and virus activity. We discovered that HBoV1 DNA persists in lymphoid germinal centers (GCs), but not in the corresponding tonsillar epithelium, and that the cell types harboring the virus are mainly naive, activated, and memory B cells and monocytes. Both viral DNA strands and both sides of the genome were detected, as well as infrequent mRNA. Moreover, we showed, in B-cell and monocyte cultures and ex vivo tonsillar B cells, that the cellular uptake of HBoV1 occurs via the Fc receptor (FcγRII) through antibody-dependent enhancement (ADE). This resulted in viral mRNA transcription, known to occur exclusively from double-stranded DNA in the nucleus, however, with no detectable productive replication. Confocal imaging with fluorescent virus-like particles moreover disclosed endocytosis. To which extent the active HBoV1 GC persistence has a role in chronic inflammation or B-cell maturation disturbances, and whether the virus can be reactivated, will be interesting topics for forthcoming studies.
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spelling pubmed-78580592021-02-05 Persistence of Human Bocavirus 1 in Tonsillar Germinal Centers and Antibody-Dependent Enhancement of Infection Xu, Man Perdomo, Maria Fernanda Mattola, Salla Pyöriä, Lari Toppinen, Mari Qiu, Jianming Vihinen-Ranta, Maija Hedman, Klaus Nokso-Koivisto, Johanna Aaltonen, Leena-Maija Söderlund-Venermo, Maria mBio Research Article Human bocavirus 1 (HBoV1), a nonenveloped single-stranded DNA parvovirus, causes mild to life-threatening respiratory tract infections, acute otitis media, and encephalitis in young children. HBoV1 often persists in nasopharyngeal secretions for months, hampering diagnosis. It has also been shown to persist in pediatric palatine and adenoid tonsils, which suggests that lymphoid organs are reservoirs for virus spread; however, the tissue site and host cells remain unknown. Our aim was to determine, in healthy nonviremic children with preexisting HBoV1 immunity, the adenotonsillar persistence site(s), host cell types, and virus activity. We discovered that HBoV1 DNA persists in lymphoid germinal centers (GCs), but not in the corresponding tonsillar epithelium, and that the cell types harboring the virus are mainly naive, activated, and memory B cells and monocytes. Both viral DNA strands and both sides of the genome were detected, as well as infrequent mRNA. Moreover, we showed, in B-cell and monocyte cultures and ex vivo tonsillar B cells, that the cellular uptake of HBoV1 occurs via the Fc receptor (FcγRII) through antibody-dependent enhancement (ADE). This resulted in viral mRNA transcription, known to occur exclusively from double-stranded DNA in the nucleus, however, with no detectable productive replication. Confocal imaging with fluorescent virus-like particles moreover disclosed endocytosis. To which extent the active HBoV1 GC persistence has a role in chronic inflammation or B-cell maturation disturbances, and whether the virus can be reactivated, will be interesting topics for forthcoming studies. American Society for Microbiology 2021-02-02 /pmc/articles/PMC7858059/ /pubmed/33531399 http://dx.doi.org/10.1128/mBio.03132-20 Text en Copyright © 2021 Xu et al. https://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Research Article
Xu, Man
Perdomo, Maria Fernanda
Mattola, Salla
Pyöriä, Lari
Toppinen, Mari
Qiu, Jianming
Vihinen-Ranta, Maija
Hedman, Klaus
Nokso-Koivisto, Johanna
Aaltonen, Leena-Maija
Söderlund-Venermo, Maria
Persistence of Human Bocavirus 1 in Tonsillar Germinal Centers and Antibody-Dependent Enhancement of Infection
title Persistence of Human Bocavirus 1 in Tonsillar Germinal Centers and Antibody-Dependent Enhancement of Infection
title_full Persistence of Human Bocavirus 1 in Tonsillar Germinal Centers and Antibody-Dependent Enhancement of Infection
title_fullStr Persistence of Human Bocavirus 1 in Tonsillar Germinal Centers and Antibody-Dependent Enhancement of Infection
title_full_unstemmed Persistence of Human Bocavirus 1 in Tonsillar Germinal Centers and Antibody-Dependent Enhancement of Infection
title_short Persistence of Human Bocavirus 1 in Tonsillar Germinal Centers and Antibody-Dependent Enhancement of Infection
title_sort persistence of human bocavirus 1 in tonsillar germinal centers and antibody-dependent enhancement of infection
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7858059/
https://www.ncbi.nlm.nih.gov/pubmed/33531399
http://dx.doi.org/10.1128/mBio.03132-20
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