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Metagenomic Quantification of Genes with Internal Standards

We demonstrate that an assembly-independent and spike-in facilitated metagenomic quantification approach can be used to screen and quantify over 2,000 genes simultaneously, while delivering absolute gene concentrations comparable to those for quantitative PCR (qPCR). DNA extracted from dairy manure...

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Detalles Bibliográficos
Autores principales:	Crossette, Emily, Gumm, Jordan, Langenfeld, Kathryn, Raskin, Lutgarde, Duhaime, Melissa, Wigginton, Krista
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	American Society for Microbiology 2021
Materias:	Research Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7858063/ https://www.ncbi.nlm.nih.gov/pubmed/33531401 http://dx.doi.org/10.1128/mBio.03173-20

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