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Telomere Dysfunction in Oocytes and Embryos From Obese Mice
Maternal obesity impairs oocyte quality and embryo development. However, the potential molecular pathways remain to be explored. In the present study, we examined the effects of obesity on telomere status in oocytes and embryos obtained from mice fed with high-fat diet (HFD). Of note, telomere short...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7858262/ https://www.ncbi.nlm.nih.gov/pubmed/33553179 http://dx.doi.org/10.3389/fcell.2021.617225 |
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author | Ge, Juan Li, Congyang Sun, Hongzheng Xin, Yongan Zhu, Shuai Liu, Yuan Tang, Shoubin Han, Longsen Huang, Zhenyue Wang, Qiang |
author_facet | Ge, Juan Li, Congyang Sun, Hongzheng Xin, Yongan Zhu, Shuai Liu, Yuan Tang, Shoubin Han, Longsen Huang, Zhenyue Wang, Qiang |
author_sort | Ge, Juan |
collection | PubMed |
description | Maternal obesity impairs oocyte quality and embryo development. However, the potential molecular pathways remain to be explored. In the present study, we examined the effects of obesity on telomere status in oocytes and embryos obtained from mice fed with high-fat diet (HFD). Of note, telomere shortening was observed in both oocytes and early embryos from obese mice, as evidenced by the reduced expression of telomerase reverse transcriptase and activity of telomerase. Moreover, quantitative analysis of telomere dysfunction-induced foci (TIFs) revealed that maternal obesity induces the defective telomeres in oocytes and embryos. Meanwhile, the high frequency of aneuploidy was detected in HFD oocytes and embryos as compared to controls, accompanying with the increased incidence of apoptotic blastocysts. In conclusion, these results indicate that telomere dysfunction might be a molecular pathway mediating the effects of maternal obesity on oocyte quality and embryo development. |
format | Online Article Text |
id | pubmed-7858262 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-78582622021-02-05 Telomere Dysfunction in Oocytes and Embryos From Obese Mice Ge, Juan Li, Congyang Sun, Hongzheng Xin, Yongan Zhu, Shuai Liu, Yuan Tang, Shoubin Han, Longsen Huang, Zhenyue Wang, Qiang Front Cell Dev Biol Cell and Developmental Biology Maternal obesity impairs oocyte quality and embryo development. However, the potential molecular pathways remain to be explored. In the present study, we examined the effects of obesity on telomere status in oocytes and embryos obtained from mice fed with high-fat diet (HFD). Of note, telomere shortening was observed in both oocytes and early embryos from obese mice, as evidenced by the reduced expression of telomerase reverse transcriptase and activity of telomerase. Moreover, quantitative analysis of telomere dysfunction-induced foci (TIFs) revealed that maternal obesity induces the defective telomeres in oocytes and embryos. Meanwhile, the high frequency of aneuploidy was detected in HFD oocytes and embryos as compared to controls, accompanying with the increased incidence of apoptotic blastocysts. In conclusion, these results indicate that telomere dysfunction might be a molecular pathway mediating the effects of maternal obesity on oocyte quality and embryo development. Frontiers Media S.A. 2021-01-21 /pmc/articles/PMC7858262/ /pubmed/33553179 http://dx.doi.org/10.3389/fcell.2021.617225 Text en Copyright © 2021 Ge, Li, Sun, Xin, Zhu, Liu, Tang, Han, Huang and Wang. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Cell and Developmental Biology Ge, Juan Li, Congyang Sun, Hongzheng Xin, Yongan Zhu, Shuai Liu, Yuan Tang, Shoubin Han, Longsen Huang, Zhenyue Wang, Qiang Telomere Dysfunction in Oocytes and Embryos From Obese Mice |
title | Telomere Dysfunction in Oocytes and Embryos From Obese Mice |
title_full | Telomere Dysfunction in Oocytes and Embryos From Obese Mice |
title_fullStr | Telomere Dysfunction in Oocytes and Embryos From Obese Mice |
title_full_unstemmed | Telomere Dysfunction in Oocytes and Embryos From Obese Mice |
title_short | Telomere Dysfunction in Oocytes and Embryos From Obese Mice |
title_sort | telomere dysfunction in oocytes and embryos from obese mice |
topic | Cell and Developmental Biology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7858262/ https://www.ncbi.nlm.nih.gov/pubmed/33553179 http://dx.doi.org/10.3389/fcell.2021.617225 |
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