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Silicon nitride: a potent solid-state bioceramic inactivator of ssRNA viruses
Surface inactivation of human microbial pathogens has a long history. The Smith Papyrus (2600 ~ 2200 B.C.) described the use of copper surfaces to sterilize chest wounds and drinking water. Brass and bronze on doorknobs can discourage microbial spread in hospitals, and metal-base surface coatings ar...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Nature Publishing Group UK
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7858580/ https://www.ncbi.nlm.nih.gov/pubmed/33536558 http://dx.doi.org/10.1038/s41598-021-82608-3 |
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author | Pezzotti, Giuseppe Boschetto, Francesco Ohgitani, Eriko Fujita, Yuki Zhu, Wenliang Marin, Elia McEntire, Bryan J. Bal, B. Sonny Mazda, Osam |
author_facet | Pezzotti, Giuseppe Boschetto, Francesco Ohgitani, Eriko Fujita, Yuki Zhu, Wenliang Marin, Elia McEntire, Bryan J. Bal, B. Sonny Mazda, Osam |
author_sort | Pezzotti, Giuseppe |
collection | PubMed |
description | Surface inactivation of human microbial pathogens has a long history. The Smith Papyrus (2600 ~ 2200 B.C.) described the use of copper surfaces to sterilize chest wounds and drinking water. Brass and bronze on doorknobs can discourage microbial spread in hospitals, and metal-base surface coatings are used in hygiene-sensitive environments, both as inactivators and modulators of cellular immunity. A limitation of these approaches is that the reactive oxygen radicals (ROS) generated at metal surfaces also damage human cells by oxidizing their proteins and lipids. Silicon nitride (Si(3)N(4)) is a non-oxide ceramic compound with known surface bacterial resistance. We show here that off-stoichiometric reactions at Si(3)N(4) surfaces are also capable of inactivating different types of single-stranded RNA (ssRNA) viruses independent of whether their structure presents an envelop or not. The antiviral property of Si(3)N(4) derives from a hydrolysis reaction at its surface and the subsequent formation of reactive nitrogen species (RNS) in doses that could be metabolized by mammalian cells but are lethal to pathogens. Real-time reverse transcription (RT)-polymerase chain reaction (PCR) tests of viral RNA and in situ Raman spectroscopy suggested that the products of Si(3)N(4) hydrolysis directly react with viral proteins and RNA. Si(3)N(4) may have a role in controlling human epidemics related to ssRNA mutant viruses. |
format | Online Article Text |
id | pubmed-7858580 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-78585802021-02-04 Silicon nitride: a potent solid-state bioceramic inactivator of ssRNA viruses Pezzotti, Giuseppe Boschetto, Francesco Ohgitani, Eriko Fujita, Yuki Zhu, Wenliang Marin, Elia McEntire, Bryan J. Bal, B. Sonny Mazda, Osam Sci Rep Article Surface inactivation of human microbial pathogens has a long history. The Smith Papyrus (2600 ~ 2200 B.C.) described the use of copper surfaces to sterilize chest wounds and drinking water. Brass and bronze on doorknobs can discourage microbial spread in hospitals, and metal-base surface coatings are used in hygiene-sensitive environments, both as inactivators and modulators of cellular immunity. A limitation of these approaches is that the reactive oxygen radicals (ROS) generated at metal surfaces also damage human cells by oxidizing their proteins and lipids. Silicon nitride (Si(3)N(4)) is a non-oxide ceramic compound with known surface bacterial resistance. We show here that off-stoichiometric reactions at Si(3)N(4) surfaces are also capable of inactivating different types of single-stranded RNA (ssRNA) viruses independent of whether their structure presents an envelop or not. The antiviral property of Si(3)N(4) derives from a hydrolysis reaction at its surface and the subsequent formation of reactive nitrogen species (RNS) in doses that could be metabolized by mammalian cells but are lethal to pathogens. Real-time reverse transcription (RT)-polymerase chain reaction (PCR) tests of viral RNA and in situ Raman spectroscopy suggested that the products of Si(3)N(4) hydrolysis directly react with viral proteins and RNA. Si(3)N(4) may have a role in controlling human epidemics related to ssRNA mutant viruses. Nature Publishing Group UK 2021-02-03 /pmc/articles/PMC7858580/ /pubmed/33536558 http://dx.doi.org/10.1038/s41598-021-82608-3 Text en © The Author(s) 2021 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Pezzotti, Giuseppe Boschetto, Francesco Ohgitani, Eriko Fujita, Yuki Zhu, Wenliang Marin, Elia McEntire, Bryan J. Bal, B. Sonny Mazda, Osam Silicon nitride: a potent solid-state bioceramic inactivator of ssRNA viruses |
title | Silicon nitride: a potent solid-state bioceramic inactivator of ssRNA viruses |
title_full | Silicon nitride: a potent solid-state bioceramic inactivator of ssRNA viruses |
title_fullStr | Silicon nitride: a potent solid-state bioceramic inactivator of ssRNA viruses |
title_full_unstemmed | Silicon nitride: a potent solid-state bioceramic inactivator of ssRNA viruses |
title_short | Silicon nitride: a potent solid-state bioceramic inactivator of ssRNA viruses |
title_sort | silicon nitride: a potent solid-state bioceramic inactivator of ssrna viruses |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7858580/ https://www.ncbi.nlm.nih.gov/pubmed/33536558 http://dx.doi.org/10.1038/s41598-021-82608-3 |
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