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B-cells expressing NgR1 and NgR3 are localized to EAE-induced inflammatory infiltrates and are stimulated by BAFF

We have previously reported evidence that Nogo-A activation of Nogo-receptor 1 (NgR1) can drive axonal dystrophy during the neurological progression of experimental autoimmune encephalomyelitis (EAE). However, the B-cell activating factor (BAFF/BlyS) may also be an important ligand of NgR during neu...

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Autores principales: Bakhuraysah, Maha M., Theotokis, Paschalis, Lee, Jae Young, Alrehaili, Amani A., Aui, Pei-Mun, Figgett, William A., Azari, Michael F., Abou-Afech, John-Paul, Mackay, Fabienne, Siatskas, Christopher, Alderuccio, Frank, Strittmatter, Stephen M., Grigoriadis, Nikolaos, Petratos, Steven
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7858582/
https://www.ncbi.nlm.nih.gov/pubmed/33536561
http://dx.doi.org/10.1038/s41598-021-82346-6
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author Bakhuraysah, Maha M.
Theotokis, Paschalis
Lee, Jae Young
Alrehaili, Amani A.
Aui, Pei-Mun
Figgett, William A.
Azari, Michael F.
Abou-Afech, John-Paul
Mackay, Fabienne
Siatskas, Christopher
Alderuccio, Frank
Strittmatter, Stephen M.
Grigoriadis, Nikolaos
Petratos, Steven
author_facet Bakhuraysah, Maha M.
Theotokis, Paschalis
Lee, Jae Young
Alrehaili, Amani A.
Aui, Pei-Mun
Figgett, William A.
Azari, Michael F.
Abou-Afech, John-Paul
Mackay, Fabienne
Siatskas, Christopher
Alderuccio, Frank
Strittmatter, Stephen M.
Grigoriadis, Nikolaos
Petratos, Steven
author_sort Bakhuraysah, Maha M.
collection PubMed
description We have previously reported evidence that Nogo-A activation of Nogo-receptor 1 (NgR1) can drive axonal dystrophy during the neurological progression of experimental autoimmune encephalomyelitis (EAE). However, the B-cell activating factor (BAFF/BlyS) may also be an important ligand of NgR during neuroinflammation. In the current study we define that NgR1 and its homologs may contribute to immune cell signaling during EAE. Meningeal B-cells expressing NgR1 and NgR3 were identified within the lumbosacral spinal cords of ngr1(+/+) EAE-induced mice at clinical score 1. Furthermore, increased secretion of immunoglobulins that bound to central nervous system myelin were shown to be generated from isolated NgR1- and NgR3-expressing B-cells of ngr1(+/+) EAE-induced mice. In vitro BAFF stimulation of NgR1- and NgR3-expressing B cells, directed them into the cell cycle DNA synthesis phase. However, when we antagonized BAFF signaling by co-incubation with recombinant BAFF-R, NgR1-Fc, or NgR3 peptides, the B cells remained in the G0/G1 phase. The data suggest that B cells express NgR1 and NgR3 during EAE, being localized to infiltrates of the meninges and that their regulation is governed by BAFF signaling.
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spelling pubmed-78585822021-02-04 B-cells expressing NgR1 and NgR3 are localized to EAE-induced inflammatory infiltrates and are stimulated by BAFF Bakhuraysah, Maha M. Theotokis, Paschalis Lee, Jae Young Alrehaili, Amani A. Aui, Pei-Mun Figgett, William A. Azari, Michael F. Abou-Afech, John-Paul Mackay, Fabienne Siatskas, Christopher Alderuccio, Frank Strittmatter, Stephen M. Grigoriadis, Nikolaos Petratos, Steven Sci Rep Article We have previously reported evidence that Nogo-A activation of Nogo-receptor 1 (NgR1) can drive axonal dystrophy during the neurological progression of experimental autoimmune encephalomyelitis (EAE). However, the B-cell activating factor (BAFF/BlyS) may also be an important ligand of NgR during neuroinflammation. In the current study we define that NgR1 and its homologs may contribute to immune cell signaling during EAE. Meningeal B-cells expressing NgR1 and NgR3 were identified within the lumbosacral spinal cords of ngr1(+/+) EAE-induced mice at clinical score 1. Furthermore, increased secretion of immunoglobulins that bound to central nervous system myelin were shown to be generated from isolated NgR1- and NgR3-expressing B-cells of ngr1(+/+) EAE-induced mice. In vitro BAFF stimulation of NgR1- and NgR3-expressing B cells, directed them into the cell cycle DNA synthesis phase. However, when we antagonized BAFF signaling by co-incubation with recombinant BAFF-R, NgR1-Fc, or NgR3 peptides, the B cells remained in the G0/G1 phase. The data suggest that B cells express NgR1 and NgR3 during EAE, being localized to infiltrates of the meninges and that their regulation is governed by BAFF signaling. Nature Publishing Group UK 2021-02-03 /pmc/articles/PMC7858582/ /pubmed/33536561 http://dx.doi.org/10.1038/s41598-021-82346-6 Text en © Crown 2021 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Bakhuraysah, Maha M.
Theotokis, Paschalis
Lee, Jae Young
Alrehaili, Amani A.
Aui, Pei-Mun
Figgett, William A.
Azari, Michael F.
Abou-Afech, John-Paul
Mackay, Fabienne
Siatskas, Christopher
Alderuccio, Frank
Strittmatter, Stephen M.
Grigoriadis, Nikolaos
Petratos, Steven
B-cells expressing NgR1 and NgR3 are localized to EAE-induced inflammatory infiltrates and are stimulated by BAFF
title B-cells expressing NgR1 and NgR3 are localized to EAE-induced inflammatory infiltrates and are stimulated by BAFF
title_full B-cells expressing NgR1 and NgR3 are localized to EAE-induced inflammatory infiltrates and are stimulated by BAFF
title_fullStr B-cells expressing NgR1 and NgR3 are localized to EAE-induced inflammatory infiltrates and are stimulated by BAFF
title_full_unstemmed B-cells expressing NgR1 and NgR3 are localized to EAE-induced inflammatory infiltrates and are stimulated by BAFF
title_short B-cells expressing NgR1 and NgR3 are localized to EAE-induced inflammatory infiltrates and are stimulated by BAFF
title_sort b-cells expressing ngr1 and ngr3 are localized to eae-induced inflammatory infiltrates and are stimulated by baff
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7858582/
https://www.ncbi.nlm.nih.gov/pubmed/33536561
http://dx.doi.org/10.1038/s41598-021-82346-6
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