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Nephropathogenic Infectious Bronchitis Virus Infection Altered the Metabolome Profile and Immune Function of the Bursa of Fabricius in Chicken

Infectious bronchitis is a highly contagious, acute viral respiratory disease of chickens, regardless of the strain, and its infection may lead to considerable economic losses to the poultry industry. New nephropathogenic infectious bronchitis virus (NIBV) strains have increasingly emerged in recent...

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Autores principales: Kuang, Jun, Xu, Puzhi, Shi, Yan, Yang, Yitian, Liu, Ping, Chen, Shupeng, Zhou, Changming, Li, Guyue, Zhuang, Yu, Hu, Ruiming, Hu, Guoliang, Guo, Xiaoquan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7858655/
https://www.ncbi.nlm.nih.gov/pubmed/33553290
http://dx.doi.org/10.3389/fvets.2020.628270
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author Kuang, Jun
Xu, Puzhi
Shi, Yan
Yang, Yitian
Liu, Ping
Chen, Shupeng
Zhou, Changming
Li, Guyue
Zhuang, Yu
Hu, Ruiming
Hu, Guoliang
Guo, Xiaoquan
author_facet Kuang, Jun
Xu, Puzhi
Shi, Yan
Yang, Yitian
Liu, Ping
Chen, Shupeng
Zhou, Changming
Li, Guyue
Zhuang, Yu
Hu, Ruiming
Hu, Guoliang
Guo, Xiaoquan
author_sort Kuang, Jun
collection PubMed
description Infectious bronchitis is a highly contagious, acute viral respiratory disease of chickens, regardless of the strain, and its infection may lead to considerable economic losses to the poultry industry. New nephropathogenic infectious bronchitis virus (NIBV) strains have increasingly emerged in recent years; hence, evaluating their infection-influenced immune function changes and the alteration of metabolite profiling is important. Initially, chickens were randomly distributed into two groups: the control group (Con) and the disease group (Dis). Here, the partial cytokines were examined, and the metabolome alterations of the bursa of Fabricius (BF) in NIBV infections in chickens were profiled by gas chromatography time-of-flight/mass spectrometry (GC-TOF/MS). The results revealed that the NIBV infection promotes the mRNA expression of inflammatory cytokines. Metabolic profile analysis indicated that clustering differed between the two groups and there were 75 significantly different metabolites detected between the two groups, suggesting that the host metabolism was significantly changed by NIBV infection. Notably, the following 12 metabolites were identified as the potential biomarkers: 3-phenyllactic acid, 2-deoxytetronic acid, aminomalonic acid, malonamide 5, uric acid, arachidonic acid, 2-methylglutaric acid, linoleic acid, ethanolamine, stearic acid, N-alpha-acetyl-l-ornithine, and O-acetylserine. Furthermore, the results of the correlation analysis showed that a strong correlation existed between metabolic biomarkers and inflammatory cytokines. Our results describe an immune and metabolic profile for the BF of chickens when infected with NIBV and provide new biomarkers of NIBV infection as potential targets and indicators of indicating therapeutic efficacy.
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spelling pubmed-78586552021-02-05 Nephropathogenic Infectious Bronchitis Virus Infection Altered the Metabolome Profile and Immune Function of the Bursa of Fabricius in Chicken Kuang, Jun Xu, Puzhi Shi, Yan Yang, Yitian Liu, Ping Chen, Shupeng Zhou, Changming Li, Guyue Zhuang, Yu Hu, Ruiming Hu, Guoliang Guo, Xiaoquan Front Vet Sci Veterinary Science Infectious bronchitis is a highly contagious, acute viral respiratory disease of chickens, regardless of the strain, and its infection may lead to considerable economic losses to the poultry industry. New nephropathogenic infectious bronchitis virus (NIBV) strains have increasingly emerged in recent years; hence, evaluating their infection-influenced immune function changes and the alteration of metabolite profiling is important. Initially, chickens were randomly distributed into two groups: the control group (Con) and the disease group (Dis). Here, the partial cytokines were examined, and the metabolome alterations of the bursa of Fabricius (BF) in NIBV infections in chickens were profiled by gas chromatography time-of-flight/mass spectrometry (GC-TOF/MS). The results revealed that the NIBV infection promotes the mRNA expression of inflammatory cytokines. Metabolic profile analysis indicated that clustering differed between the two groups and there were 75 significantly different metabolites detected between the two groups, suggesting that the host metabolism was significantly changed by NIBV infection. Notably, the following 12 metabolites were identified as the potential biomarkers: 3-phenyllactic acid, 2-deoxytetronic acid, aminomalonic acid, malonamide 5, uric acid, arachidonic acid, 2-methylglutaric acid, linoleic acid, ethanolamine, stearic acid, N-alpha-acetyl-l-ornithine, and O-acetylserine. Furthermore, the results of the correlation analysis showed that a strong correlation existed between metabolic biomarkers and inflammatory cytokines. Our results describe an immune and metabolic profile for the BF of chickens when infected with NIBV and provide new biomarkers of NIBV infection as potential targets and indicators of indicating therapeutic efficacy. Frontiers Media S.A. 2021-01-21 /pmc/articles/PMC7858655/ /pubmed/33553290 http://dx.doi.org/10.3389/fvets.2020.628270 Text en Copyright © 2021 Kuang, Xu, Shi, Yang, Liu, Chen, Zhou, Li, Zhuang, Hu, Hu and Guo. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Veterinary Science
Kuang, Jun
Xu, Puzhi
Shi, Yan
Yang, Yitian
Liu, Ping
Chen, Shupeng
Zhou, Changming
Li, Guyue
Zhuang, Yu
Hu, Ruiming
Hu, Guoliang
Guo, Xiaoquan
Nephropathogenic Infectious Bronchitis Virus Infection Altered the Metabolome Profile and Immune Function of the Bursa of Fabricius in Chicken
title Nephropathogenic Infectious Bronchitis Virus Infection Altered the Metabolome Profile and Immune Function of the Bursa of Fabricius in Chicken
title_full Nephropathogenic Infectious Bronchitis Virus Infection Altered the Metabolome Profile and Immune Function of the Bursa of Fabricius in Chicken
title_fullStr Nephropathogenic Infectious Bronchitis Virus Infection Altered the Metabolome Profile and Immune Function of the Bursa of Fabricius in Chicken
title_full_unstemmed Nephropathogenic Infectious Bronchitis Virus Infection Altered the Metabolome Profile and Immune Function of the Bursa of Fabricius in Chicken
title_short Nephropathogenic Infectious Bronchitis Virus Infection Altered the Metabolome Profile and Immune Function of the Bursa of Fabricius in Chicken
title_sort nephropathogenic infectious bronchitis virus infection altered the metabolome profile and immune function of the bursa of fabricius in chicken
topic Veterinary Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7858655/
https://www.ncbi.nlm.nih.gov/pubmed/33553290
http://dx.doi.org/10.3389/fvets.2020.628270
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