Cargando…
Nephropathogenic Infectious Bronchitis Virus Infection Altered the Metabolome Profile and Immune Function of the Bursa of Fabricius in Chicken
Infectious bronchitis is a highly contagious, acute viral respiratory disease of chickens, regardless of the strain, and its infection may lead to considerable economic losses to the poultry industry. New nephropathogenic infectious bronchitis virus (NIBV) strains have increasingly emerged in recent...
Autores principales: | , , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2021
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7858655/ https://www.ncbi.nlm.nih.gov/pubmed/33553290 http://dx.doi.org/10.3389/fvets.2020.628270 |
_version_ | 1783646645305475072 |
---|---|
author | Kuang, Jun Xu, Puzhi Shi, Yan Yang, Yitian Liu, Ping Chen, Shupeng Zhou, Changming Li, Guyue Zhuang, Yu Hu, Ruiming Hu, Guoliang Guo, Xiaoquan |
author_facet | Kuang, Jun Xu, Puzhi Shi, Yan Yang, Yitian Liu, Ping Chen, Shupeng Zhou, Changming Li, Guyue Zhuang, Yu Hu, Ruiming Hu, Guoliang Guo, Xiaoquan |
author_sort | Kuang, Jun |
collection | PubMed |
description | Infectious bronchitis is a highly contagious, acute viral respiratory disease of chickens, regardless of the strain, and its infection may lead to considerable economic losses to the poultry industry. New nephropathogenic infectious bronchitis virus (NIBV) strains have increasingly emerged in recent years; hence, evaluating their infection-influenced immune function changes and the alteration of metabolite profiling is important. Initially, chickens were randomly distributed into two groups: the control group (Con) and the disease group (Dis). Here, the partial cytokines were examined, and the metabolome alterations of the bursa of Fabricius (BF) in NIBV infections in chickens were profiled by gas chromatography time-of-flight/mass spectrometry (GC-TOF/MS). The results revealed that the NIBV infection promotes the mRNA expression of inflammatory cytokines. Metabolic profile analysis indicated that clustering differed between the two groups and there were 75 significantly different metabolites detected between the two groups, suggesting that the host metabolism was significantly changed by NIBV infection. Notably, the following 12 metabolites were identified as the potential biomarkers: 3-phenyllactic acid, 2-deoxytetronic acid, aminomalonic acid, malonamide 5, uric acid, arachidonic acid, 2-methylglutaric acid, linoleic acid, ethanolamine, stearic acid, N-alpha-acetyl-l-ornithine, and O-acetylserine. Furthermore, the results of the correlation analysis showed that a strong correlation existed between metabolic biomarkers and inflammatory cytokines. Our results describe an immune and metabolic profile for the BF of chickens when infected with NIBV and provide new biomarkers of NIBV infection as potential targets and indicators of indicating therapeutic efficacy. |
format | Online Article Text |
id | pubmed-7858655 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-78586552021-02-05 Nephropathogenic Infectious Bronchitis Virus Infection Altered the Metabolome Profile and Immune Function of the Bursa of Fabricius in Chicken Kuang, Jun Xu, Puzhi Shi, Yan Yang, Yitian Liu, Ping Chen, Shupeng Zhou, Changming Li, Guyue Zhuang, Yu Hu, Ruiming Hu, Guoliang Guo, Xiaoquan Front Vet Sci Veterinary Science Infectious bronchitis is a highly contagious, acute viral respiratory disease of chickens, regardless of the strain, and its infection may lead to considerable economic losses to the poultry industry. New nephropathogenic infectious bronchitis virus (NIBV) strains have increasingly emerged in recent years; hence, evaluating their infection-influenced immune function changes and the alteration of metabolite profiling is important. Initially, chickens were randomly distributed into two groups: the control group (Con) and the disease group (Dis). Here, the partial cytokines were examined, and the metabolome alterations of the bursa of Fabricius (BF) in NIBV infections in chickens were profiled by gas chromatography time-of-flight/mass spectrometry (GC-TOF/MS). The results revealed that the NIBV infection promotes the mRNA expression of inflammatory cytokines. Metabolic profile analysis indicated that clustering differed between the two groups and there were 75 significantly different metabolites detected between the two groups, suggesting that the host metabolism was significantly changed by NIBV infection. Notably, the following 12 metabolites were identified as the potential biomarkers: 3-phenyllactic acid, 2-deoxytetronic acid, aminomalonic acid, malonamide 5, uric acid, arachidonic acid, 2-methylglutaric acid, linoleic acid, ethanolamine, stearic acid, N-alpha-acetyl-l-ornithine, and O-acetylserine. Furthermore, the results of the correlation analysis showed that a strong correlation existed between metabolic biomarkers and inflammatory cytokines. Our results describe an immune and metabolic profile for the BF of chickens when infected with NIBV and provide new biomarkers of NIBV infection as potential targets and indicators of indicating therapeutic efficacy. Frontiers Media S.A. 2021-01-21 /pmc/articles/PMC7858655/ /pubmed/33553290 http://dx.doi.org/10.3389/fvets.2020.628270 Text en Copyright © 2021 Kuang, Xu, Shi, Yang, Liu, Chen, Zhou, Li, Zhuang, Hu, Hu and Guo. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Veterinary Science Kuang, Jun Xu, Puzhi Shi, Yan Yang, Yitian Liu, Ping Chen, Shupeng Zhou, Changming Li, Guyue Zhuang, Yu Hu, Ruiming Hu, Guoliang Guo, Xiaoquan Nephropathogenic Infectious Bronchitis Virus Infection Altered the Metabolome Profile and Immune Function of the Bursa of Fabricius in Chicken |
title | Nephropathogenic Infectious Bronchitis Virus Infection Altered the Metabolome Profile and Immune Function of the Bursa of Fabricius in Chicken |
title_full | Nephropathogenic Infectious Bronchitis Virus Infection Altered the Metabolome Profile and Immune Function of the Bursa of Fabricius in Chicken |
title_fullStr | Nephropathogenic Infectious Bronchitis Virus Infection Altered the Metabolome Profile and Immune Function of the Bursa of Fabricius in Chicken |
title_full_unstemmed | Nephropathogenic Infectious Bronchitis Virus Infection Altered the Metabolome Profile and Immune Function of the Bursa of Fabricius in Chicken |
title_short | Nephropathogenic Infectious Bronchitis Virus Infection Altered the Metabolome Profile and Immune Function of the Bursa of Fabricius in Chicken |
title_sort | nephropathogenic infectious bronchitis virus infection altered the metabolome profile and immune function of the bursa of fabricius in chicken |
topic | Veterinary Science |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7858655/ https://www.ncbi.nlm.nih.gov/pubmed/33553290 http://dx.doi.org/10.3389/fvets.2020.628270 |
work_keys_str_mv | AT kuangjun nephropathogenicinfectiousbronchitisvirusinfectionalteredthemetabolomeprofileandimmunefunctionofthebursaoffabriciusinchicken AT xupuzhi nephropathogenicinfectiousbronchitisvirusinfectionalteredthemetabolomeprofileandimmunefunctionofthebursaoffabriciusinchicken AT shiyan nephropathogenicinfectiousbronchitisvirusinfectionalteredthemetabolomeprofileandimmunefunctionofthebursaoffabriciusinchicken AT yangyitian nephropathogenicinfectiousbronchitisvirusinfectionalteredthemetabolomeprofileandimmunefunctionofthebursaoffabriciusinchicken AT liuping nephropathogenicinfectiousbronchitisvirusinfectionalteredthemetabolomeprofileandimmunefunctionofthebursaoffabriciusinchicken AT chenshupeng nephropathogenicinfectiousbronchitisvirusinfectionalteredthemetabolomeprofileandimmunefunctionofthebursaoffabriciusinchicken AT zhouchangming nephropathogenicinfectiousbronchitisvirusinfectionalteredthemetabolomeprofileandimmunefunctionofthebursaoffabriciusinchicken AT liguyue nephropathogenicinfectiousbronchitisvirusinfectionalteredthemetabolomeprofileandimmunefunctionofthebursaoffabriciusinchicken AT zhuangyu nephropathogenicinfectiousbronchitisvirusinfectionalteredthemetabolomeprofileandimmunefunctionofthebursaoffabriciusinchicken AT huruiming nephropathogenicinfectiousbronchitisvirusinfectionalteredthemetabolomeprofileandimmunefunctionofthebursaoffabriciusinchicken AT huguoliang nephropathogenicinfectiousbronchitisvirusinfectionalteredthemetabolomeprofileandimmunefunctionofthebursaoffabriciusinchicken AT guoxiaoquan nephropathogenicinfectiousbronchitisvirusinfectionalteredthemetabolomeprofileandimmunefunctionofthebursaoffabriciusinchicken |