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Parvimonas micra, Peptostreptococcus stomatis, Fusobacterium nucleatum and Akkermansia muciniphila as a four-bacteria biomarker panel of colorectal cancer

Dysbiosis of the gut microbiome has been associated with the pathogenesis of colorectal cancer (CRC). We profiled the microbiome of gut mucosal tissues from 18 CRC patients and 18 non-CRC controls of the UKM Medical Centre (UKMMC), Kuala Lumpur, Malaysia. The results were then validated using a spec...

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Autores principales: Osman, Muhammad Afiq, Neoh, Hui-min, Ab Mutalib, Nurul-Syakima, Chin, Siok-Fong, Mazlan, Luqman, Raja Ali, Raja Affendi, Zakaria, Andee Dzulkarnaen, Ngiu, Chai Soon, Ang, Mia Yang, Jamal, Rahman
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7859180/
https://www.ncbi.nlm.nih.gov/pubmed/33536501
http://dx.doi.org/10.1038/s41598-021-82465-0
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author Osman, Muhammad Afiq
Neoh, Hui-min
Ab Mutalib, Nurul-Syakima
Chin, Siok-Fong
Mazlan, Luqman
Raja Ali, Raja Affendi
Zakaria, Andee Dzulkarnaen
Ngiu, Chai Soon
Ang, Mia Yang
Jamal, Rahman
author_facet Osman, Muhammad Afiq
Neoh, Hui-min
Ab Mutalib, Nurul-Syakima
Chin, Siok-Fong
Mazlan, Luqman
Raja Ali, Raja Affendi
Zakaria, Andee Dzulkarnaen
Ngiu, Chai Soon
Ang, Mia Yang
Jamal, Rahman
author_sort Osman, Muhammad Afiq
collection PubMed
description Dysbiosis of the gut microbiome has been associated with the pathogenesis of colorectal cancer (CRC). We profiled the microbiome of gut mucosal tissues from 18 CRC patients and 18 non-CRC controls of the UKM Medical Centre (UKMMC), Kuala Lumpur, Malaysia. The results were then validated using a species-specific quantitative PCR in 40 CRC and 20 non-CRC tissues samples from the UMBI-UKMMC Biobank. Parvimonas micra, Fusobacterium nucleatum, Peptostreptococcus stomatis and Akkermansia muciniphila were found to be over-represented in our CRC patients compared to non-CRC controls. These four bacteria markers distinguished CRC from controls (AUROC = 0.925) in our validation cohort. We identified bacteria species significantly associated (cut-off value of > 5 fold abundance) with various CRC demographics such as ethnicity, gender and CRC staging; however, due to small sample size of the discovery cohort, these results could not be further verified in our validation cohort. In summary, Parvimonas micra, Fusobacterium nucleatum, Peptostreptococcus stomatis and Akkermansia muciniphila were enriched in our local CRC patients. Nevertheless, the roles of these bacteria in CRC initiation and progression remains to be investigated.
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spelling pubmed-78591802021-02-04 Parvimonas micra, Peptostreptococcus stomatis, Fusobacterium nucleatum and Akkermansia muciniphila as a four-bacteria biomarker panel of colorectal cancer Osman, Muhammad Afiq Neoh, Hui-min Ab Mutalib, Nurul-Syakima Chin, Siok-Fong Mazlan, Luqman Raja Ali, Raja Affendi Zakaria, Andee Dzulkarnaen Ngiu, Chai Soon Ang, Mia Yang Jamal, Rahman Sci Rep Article Dysbiosis of the gut microbiome has been associated with the pathogenesis of colorectal cancer (CRC). We profiled the microbiome of gut mucosal tissues from 18 CRC patients and 18 non-CRC controls of the UKM Medical Centre (UKMMC), Kuala Lumpur, Malaysia. The results were then validated using a species-specific quantitative PCR in 40 CRC and 20 non-CRC tissues samples from the UMBI-UKMMC Biobank. Parvimonas micra, Fusobacterium nucleatum, Peptostreptococcus stomatis and Akkermansia muciniphila were found to be over-represented in our CRC patients compared to non-CRC controls. These four bacteria markers distinguished CRC from controls (AUROC = 0.925) in our validation cohort. We identified bacteria species significantly associated (cut-off value of > 5 fold abundance) with various CRC demographics such as ethnicity, gender and CRC staging; however, due to small sample size of the discovery cohort, these results could not be further verified in our validation cohort. In summary, Parvimonas micra, Fusobacterium nucleatum, Peptostreptococcus stomatis and Akkermansia muciniphila were enriched in our local CRC patients. Nevertheless, the roles of these bacteria in CRC initiation and progression remains to be investigated. Nature Publishing Group UK 2021-02-03 /pmc/articles/PMC7859180/ /pubmed/33536501 http://dx.doi.org/10.1038/s41598-021-82465-0 Text en © The Author(s) 2021 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Osman, Muhammad Afiq
Neoh, Hui-min
Ab Mutalib, Nurul-Syakima
Chin, Siok-Fong
Mazlan, Luqman
Raja Ali, Raja Affendi
Zakaria, Andee Dzulkarnaen
Ngiu, Chai Soon
Ang, Mia Yang
Jamal, Rahman
Parvimonas micra, Peptostreptococcus stomatis, Fusobacterium nucleatum and Akkermansia muciniphila as a four-bacteria biomarker panel of colorectal cancer
title Parvimonas micra, Peptostreptococcus stomatis, Fusobacterium nucleatum and Akkermansia muciniphila as a four-bacteria biomarker panel of colorectal cancer
title_full Parvimonas micra, Peptostreptococcus stomatis, Fusobacterium nucleatum and Akkermansia muciniphila as a four-bacteria biomarker panel of colorectal cancer
title_fullStr Parvimonas micra, Peptostreptococcus stomatis, Fusobacterium nucleatum and Akkermansia muciniphila as a four-bacteria biomarker panel of colorectal cancer
title_full_unstemmed Parvimonas micra, Peptostreptococcus stomatis, Fusobacterium nucleatum and Akkermansia muciniphila as a four-bacteria biomarker panel of colorectal cancer
title_short Parvimonas micra, Peptostreptococcus stomatis, Fusobacterium nucleatum and Akkermansia muciniphila as a four-bacteria biomarker panel of colorectal cancer
title_sort parvimonas micra, peptostreptococcus stomatis, fusobacterium nucleatum and akkermansia muciniphila as a four-bacteria biomarker panel of colorectal cancer
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7859180/
https://www.ncbi.nlm.nih.gov/pubmed/33536501
http://dx.doi.org/10.1038/s41598-021-82465-0
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