Cargando…
SRSFs mediate the function of AR in the ovarian granulosa cells of patients with PCOS
Ovarian hyperandrogenism is one of the characteristics of polycystic ovary syndrome (PCOS) and androgen receptor (AR) in ovarian granulosa cells (GCs) functions as an important element to the accumulation of androgens. This study verified the existence of alternative splicing variant of AR (AR-SVs)...
Autores principales: | , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Chongqing Medical University
2019
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7859457/ https://www.ncbi.nlm.nih.gov/pubmed/33569518 http://dx.doi.org/10.1016/j.gendis.2019.09.005 |
_version_ | 1783646737859084288 |
---|---|
author | Luo, Jing Ye, Hong Hao, Lijuan Sun, Yixuan Li, Ruohan Li, Yanxi Yang, Zhu |
author_facet | Luo, Jing Ye, Hong Hao, Lijuan Sun, Yixuan Li, Ruohan Li, Yanxi Yang, Zhu |
author_sort | Luo, Jing |
collection | PubMed |
description | Ovarian hyperandrogenism is one of the characteristics of polycystic ovary syndrome (PCOS) and androgen receptor (AR) in ovarian granulosa cells (GCs) functions as an important element to the accumulation of androgens. This study verified the existence of alternative splicing variant of AR (AR-SVs) in the GCs of PCOS patients and found that the function of AR decreased significantly in the presence of AR-SVs. And compared to the normal individuals, the expression of Serine/arginine-rich splicing factor 2(SRSF2) was higher and the expression of SRSF3 was lower in the GCs of patients with AR-SVs. More importantly, we found that the expression of SRSF2 was inhibited and that the expression of AR was decreased after the successful upregulation of miRNA-183, and testostrone (T) concentrations in the culture medium were increased. The results also showed that the expression of SRSF3 decreased when miRNA-124 was successfully upregulated, while the expression of AR significantly increased; however, the function of AR was also inhibited when T concentration in the culture medium was increased. This study has proved that SRSFs are regulated by corresponding miRNAs, and the altered expression of SRSFs interferenced the alternative splicing process of AR and ultimately decreased the function of AR, leading to the accumulation of androgens in the ovary. |
format | Online Article Text |
id | pubmed-7859457 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Chongqing Medical University |
record_format | MEDLINE/PubMed |
spelling | pubmed-78594572021-02-09 SRSFs mediate the function of AR in the ovarian granulosa cells of patients with PCOS Luo, Jing Ye, Hong Hao, Lijuan Sun, Yixuan Li, Ruohan Li, Yanxi Yang, Zhu Genes Dis Full Length Article Ovarian hyperandrogenism is one of the characteristics of polycystic ovary syndrome (PCOS) and androgen receptor (AR) in ovarian granulosa cells (GCs) functions as an important element to the accumulation of androgens. This study verified the existence of alternative splicing variant of AR (AR-SVs) in the GCs of PCOS patients and found that the function of AR decreased significantly in the presence of AR-SVs. And compared to the normal individuals, the expression of Serine/arginine-rich splicing factor 2(SRSF2) was higher and the expression of SRSF3 was lower in the GCs of patients with AR-SVs. More importantly, we found that the expression of SRSF2 was inhibited and that the expression of AR was decreased after the successful upregulation of miRNA-183, and testostrone (T) concentrations in the culture medium were increased. The results also showed that the expression of SRSF3 decreased when miRNA-124 was successfully upregulated, while the expression of AR significantly increased; however, the function of AR was also inhibited when T concentration in the culture medium was increased. This study has proved that SRSFs are regulated by corresponding miRNAs, and the altered expression of SRSFs interferenced the alternative splicing process of AR and ultimately decreased the function of AR, leading to the accumulation of androgens in the ovary. Chongqing Medical University 2019-09-17 /pmc/articles/PMC7859457/ /pubmed/33569518 http://dx.doi.org/10.1016/j.gendis.2019.09.005 Text en © 2019 Chongqing Medical University. Production and hosting by Elsevier B.V. http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
spellingShingle | Full Length Article Luo, Jing Ye, Hong Hao, Lijuan Sun, Yixuan Li, Ruohan Li, Yanxi Yang, Zhu SRSFs mediate the function of AR in the ovarian granulosa cells of patients with PCOS |
title | SRSFs mediate the function of AR in the ovarian granulosa cells of patients with PCOS |
title_full | SRSFs mediate the function of AR in the ovarian granulosa cells of patients with PCOS |
title_fullStr | SRSFs mediate the function of AR in the ovarian granulosa cells of patients with PCOS |
title_full_unstemmed | SRSFs mediate the function of AR in the ovarian granulosa cells of patients with PCOS |
title_short | SRSFs mediate the function of AR in the ovarian granulosa cells of patients with PCOS |
title_sort | srsfs mediate the function of ar in the ovarian granulosa cells of patients with pcos |
topic | Full Length Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7859457/ https://www.ncbi.nlm.nih.gov/pubmed/33569518 http://dx.doi.org/10.1016/j.gendis.2019.09.005 |
work_keys_str_mv | AT luojing srsfsmediatethefunctionofarintheovariangranulosacellsofpatientswithpcos AT yehong srsfsmediatethefunctionofarintheovariangranulosacellsofpatientswithpcos AT haolijuan srsfsmediatethefunctionofarintheovariangranulosacellsofpatientswithpcos AT sunyixuan srsfsmediatethefunctionofarintheovariangranulosacellsofpatientswithpcos AT liruohan srsfsmediatethefunctionofarintheovariangranulosacellsofpatientswithpcos AT liyanxi srsfsmediatethefunctionofarintheovariangranulosacellsofpatientswithpcos AT yangzhu srsfsmediatethefunctionofarintheovariangranulosacellsofpatientswithpcos |