Cargando…

Multispectral optoacoustic tomography of peripheral arterial disease based on muscle hemoglobin gradients—a pilot clinical study

BACKGROUND: Current imaging assessment of peripheral artery disease (PAD) relies on anatomical cross-sectional visualizations of the affected arteries. Multispectral optoacoustic tomography (MSOT) is a novel molecular imaging technique that provides direct and label-free visualizations of soft tissu...

Descripción completa

Detalles Bibliográficos
Autores principales: Karlas, Angelos, Masthoff, Max, Kallmayer, Michael, Helfen, Anne, Bariotakis, Michail, Fasoula, Nikolina Alexia, Schäfers, Michael, Seidensticker, Max, Eckstein, Hans-Henning, Ntziachristos, Vasilis, Wildgruber, Moritz
Formato: Online Artículo Texto
Lenguaje:English
Publicado: AME Publishing Company 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7859778/
https://www.ncbi.nlm.nih.gov/pubmed/33553329
http://dx.doi.org/10.21037/atm-20-3321
_version_ 1783646809689686016
author Karlas, Angelos
Masthoff, Max
Kallmayer, Michael
Helfen, Anne
Bariotakis, Michail
Fasoula, Nikolina Alexia
Schäfers, Michael
Seidensticker, Max
Eckstein, Hans-Henning
Ntziachristos, Vasilis
Wildgruber, Moritz
author_facet Karlas, Angelos
Masthoff, Max
Kallmayer, Michael
Helfen, Anne
Bariotakis, Michail
Fasoula, Nikolina Alexia
Schäfers, Michael
Seidensticker, Max
Eckstein, Hans-Henning
Ntziachristos, Vasilis
Wildgruber, Moritz
author_sort Karlas, Angelos
collection PubMed
description BACKGROUND: Current imaging assessment of peripheral artery disease (PAD) relies on anatomical cross-sectional visualizations of the affected arteries. Multispectral optoacoustic tomography (MSOT) is a novel molecular imaging technique that provides direct and label-free visualizations of soft tissue perfusion and oxygenation. METHODS: MSOT was prospectively assessed in a pilot trial in healthy volunteers (group n(1)=4, mean age 31, 50% male and group n(3)=4, mean age 37.3, 75% male) and patients with intermittent claudication (group n(2)=4, mean age 72, 75% male, PAD stage IIb). We conducted cuff-induced ischemia (group n(1)) and resting state measurements (groups n(2) and n(3)) over the calf region. Spatially resolved mapping of oxygenated (HbO(2)), deoxygenated (Hb) and total (THb) hemoglobin, as well as oxygen saturation (SO(2)), were measured via hand-held hybrid MSOT-Ultrasound based purely on hemoglobin contrast. RESULTS: Calf measurements in healthy volunteers revealed distinct dynamics for HbO(2), Hb, THb and SO(2) under cuff-induced ischemia. HbO(2), THb and SO(2) levels were significantly impaired in PAD patients compared to healthy volunteers (P<0.05 for all parameters). Revascularization led to significant improvements in HbO(2) of the affected limb. CONCLUSIONS: Clinical MSOT allows for non-invasive, label-free and real-time imaging of muscle oxygenation in health and disease with implications for diagnostics and therapy assessment in PAD.
format Online
Article
Text
id pubmed-7859778
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher AME Publishing Company
record_format MEDLINE/PubMed
spelling pubmed-78597782021-02-05 Multispectral optoacoustic tomography of peripheral arterial disease based on muscle hemoglobin gradients—a pilot clinical study Karlas, Angelos Masthoff, Max Kallmayer, Michael Helfen, Anne Bariotakis, Michail Fasoula, Nikolina Alexia Schäfers, Michael Seidensticker, Max Eckstein, Hans-Henning Ntziachristos, Vasilis Wildgruber, Moritz Ann Transl Med Original Article BACKGROUND: Current imaging assessment of peripheral artery disease (PAD) relies on anatomical cross-sectional visualizations of the affected arteries. Multispectral optoacoustic tomography (MSOT) is a novel molecular imaging technique that provides direct and label-free visualizations of soft tissue perfusion and oxygenation. METHODS: MSOT was prospectively assessed in a pilot trial in healthy volunteers (group n(1)=4, mean age 31, 50% male and group n(3)=4, mean age 37.3, 75% male) and patients with intermittent claudication (group n(2)=4, mean age 72, 75% male, PAD stage IIb). We conducted cuff-induced ischemia (group n(1)) and resting state measurements (groups n(2) and n(3)) over the calf region. Spatially resolved mapping of oxygenated (HbO(2)), deoxygenated (Hb) and total (THb) hemoglobin, as well as oxygen saturation (SO(2)), were measured via hand-held hybrid MSOT-Ultrasound based purely on hemoglobin contrast. RESULTS: Calf measurements in healthy volunteers revealed distinct dynamics for HbO(2), Hb, THb and SO(2) under cuff-induced ischemia. HbO(2), THb and SO(2) levels were significantly impaired in PAD patients compared to healthy volunteers (P<0.05 for all parameters). Revascularization led to significant improvements in HbO(2) of the affected limb. CONCLUSIONS: Clinical MSOT allows for non-invasive, label-free and real-time imaging of muscle oxygenation in health and disease with implications for diagnostics and therapy assessment in PAD. AME Publishing Company 2021-01 /pmc/articles/PMC7859778/ /pubmed/33553329 http://dx.doi.org/10.21037/atm-20-3321 Text en 2021 Annals of Translational Medicine. All rights reserved. https://creativecommons.org/licenses/by-nc-nd/4.0/Open Access Statement: This is an Open Access article distributed in accordance with the Creative Commons Attribution-NonCommercial-NoDerivs 4.0 International License (CC BY-NC-ND 4.0), which permits the non-commercial replication and distribution of the article with the strict proviso that no changes or edits are made and the original work is properly cited (including links to both the formal publication through the relevant DOI and the license). See: https://creativecommons.org/licenses/by-nc-nd/4.0 (https://creativecommons.org/licenses/by-nc-nd/4.0/) .
spellingShingle Original Article
Karlas, Angelos
Masthoff, Max
Kallmayer, Michael
Helfen, Anne
Bariotakis, Michail
Fasoula, Nikolina Alexia
Schäfers, Michael
Seidensticker, Max
Eckstein, Hans-Henning
Ntziachristos, Vasilis
Wildgruber, Moritz
Multispectral optoacoustic tomography of peripheral arterial disease based on muscle hemoglobin gradients—a pilot clinical study
title Multispectral optoacoustic tomography of peripheral arterial disease based on muscle hemoglobin gradients—a pilot clinical study
title_full Multispectral optoacoustic tomography of peripheral arterial disease based on muscle hemoglobin gradients—a pilot clinical study
title_fullStr Multispectral optoacoustic tomography of peripheral arterial disease based on muscle hemoglobin gradients—a pilot clinical study
title_full_unstemmed Multispectral optoacoustic tomography of peripheral arterial disease based on muscle hemoglobin gradients—a pilot clinical study
title_short Multispectral optoacoustic tomography of peripheral arterial disease based on muscle hemoglobin gradients—a pilot clinical study
title_sort multispectral optoacoustic tomography of peripheral arterial disease based on muscle hemoglobin gradients—a pilot clinical study
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7859778/
https://www.ncbi.nlm.nih.gov/pubmed/33553329
http://dx.doi.org/10.21037/atm-20-3321
work_keys_str_mv AT karlasangelos multispectraloptoacoustictomographyofperipheralarterialdiseasebasedonmusclehemoglobingradientsapilotclinicalstudy
AT masthoffmax multispectraloptoacoustictomographyofperipheralarterialdiseasebasedonmusclehemoglobingradientsapilotclinicalstudy
AT kallmayermichael multispectraloptoacoustictomographyofperipheralarterialdiseasebasedonmusclehemoglobingradientsapilotclinicalstudy
AT helfenanne multispectraloptoacoustictomographyofperipheralarterialdiseasebasedonmusclehemoglobingradientsapilotclinicalstudy
AT bariotakismichail multispectraloptoacoustictomographyofperipheralarterialdiseasebasedonmusclehemoglobingradientsapilotclinicalstudy
AT fasoulanikolinaalexia multispectraloptoacoustictomographyofperipheralarterialdiseasebasedonmusclehemoglobingradientsapilotclinicalstudy
AT schafersmichael multispectraloptoacoustictomographyofperipheralarterialdiseasebasedonmusclehemoglobingradientsapilotclinicalstudy
AT seidenstickermax multispectraloptoacoustictomographyofperipheralarterialdiseasebasedonmusclehemoglobingradientsapilotclinicalstudy
AT ecksteinhanshenning multispectraloptoacoustictomographyofperipheralarterialdiseasebasedonmusclehemoglobingradientsapilotclinicalstudy
AT ntziachristosvasilis multispectraloptoacoustictomographyofperipheralarterialdiseasebasedonmusclehemoglobingradientsapilotclinicalstudy
AT wildgrubermoritz multispectraloptoacoustictomographyofperipheralarterialdiseasebasedonmusclehemoglobingradientsapilotclinicalstudy