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Multispectral optoacoustic tomography of peripheral arterial disease based on muscle hemoglobin gradients—a pilot clinical study
BACKGROUND: Current imaging assessment of peripheral artery disease (PAD) relies on anatomical cross-sectional visualizations of the affected arteries. Multispectral optoacoustic tomography (MSOT) is a novel molecular imaging technique that provides direct and label-free visualizations of soft tissu...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
AME Publishing Company
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7859778/ https://www.ncbi.nlm.nih.gov/pubmed/33553329 http://dx.doi.org/10.21037/atm-20-3321 |
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author | Karlas, Angelos Masthoff, Max Kallmayer, Michael Helfen, Anne Bariotakis, Michail Fasoula, Nikolina Alexia Schäfers, Michael Seidensticker, Max Eckstein, Hans-Henning Ntziachristos, Vasilis Wildgruber, Moritz |
author_facet | Karlas, Angelos Masthoff, Max Kallmayer, Michael Helfen, Anne Bariotakis, Michail Fasoula, Nikolina Alexia Schäfers, Michael Seidensticker, Max Eckstein, Hans-Henning Ntziachristos, Vasilis Wildgruber, Moritz |
author_sort | Karlas, Angelos |
collection | PubMed |
description | BACKGROUND: Current imaging assessment of peripheral artery disease (PAD) relies on anatomical cross-sectional visualizations of the affected arteries. Multispectral optoacoustic tomography (MSOT) is a novel molecular imaging technique that provides direct and label-free visualizations of soft tissue perfusion and oxygenation. METHODS: MSOT was prospectively assessed in a pilot trial in healthy volunteers (group n(1)=4, mean age 31, 50% male and group n(3)=4, mean age 37.3, 75% male) and patients with intermittent claudication (group n(2)=4, mean age 72, 75% male, PAD stage IIb). We conducted cuff-induced ischemia (group n(1)) and resting state measurements (groups n(2) and n(3)) over the calf region. Spatially resolved mapping of oxygenated (HbO(2)), deoxygenated (Hb) and total (THb) hemoglobin, as well as oxygen saturation (SO(2)), were measured via hand-held hybrid MSOT-Ultrasound based purely on hemoglobin contrast. RESULTS: Calf measurements in healthy volunteers revealed distinct dynamics for HbO(2), Hb, THb and SO(2) under cuff-induced ischemia. HbO(2), THb and SO(2) levels were significantly impaired in PAD patients compared to healthy volunteers (P<0.05 for all parameters). Revascularization led to significant improvements in HbO(2) of the affected limb. CONCLUSIONS: Clinical MSOT allows for non-invasive, label-free and real-time imaging of muscle oxygenation in health and disease with implications for diagnostics and therapy assessment in PAD. |
format | Online Article Text |
id | pubmed-7859778 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | AME Publishing Company |
record_format | MEDLINE/PubMed |
spelling | pubmed-78597782021-02-05 Multispectral optoacoustic tomography of peripheral arterial disease based on muscle hemoglobin gradients—a pilot clinical study Karlas, Angelos Masthoff, Max Kallmayer, Michael Helfen, Anne Bariotakis, Michail Fasoula, Nikolina Alexia Schäfers, Michael Seidensticker, Max Eckstein, Hans-Henning Ntziachristos, Vasilis Wildgruber, Moritz Ann Transl Med Original Article BACKGROUND: Current imaging assessment of peripheral artery disease (PAD) relies on anatomical cross-sectional visualizations of the affected arteries. Multispectral optoacoustic tomography (MSOT) is a novel molecular imaging technique that provides direct and label-free visualizations of soft tissue perfusion and oxygenation. METHODS: MSOT was prospectively assessed in a pilot trial in healthy volunteers (group n(1)=4, mean age 31, 50% male and group n(3)=4, mean age 37.3, 75% male) and patients with intermittent claudication (group n(2)=4, mean age 72, 75% male, PAD stage IIb). We conducted cuff-induced ischemia (group n(1)) and resting state measurements (groups n(2) and n(3)) over the calf region. Spatially resolved mapping of oxygenated (HbO(2)), deoxygenated (Hb) and total (THb) hemoglobin, as well as oxygen saturation (SO(2)), were measured via hand-held hybrid MSOT-Ultrasound based purely on hemoglobin contrast. RESULTS: Calf measurements in healthy volunteers revealed distinct dynamics for HbO(2), Hb, THb and SO(2) under cuff-induced ischemia. HbO(2), THb and SO(2) levels were significantly impaired in PAD patients compared to healthy volunteers (P<0.05 for all parameters). Revascularization led to significant improvements in HbO(2) of the affected limb. CONCLUSIONS: Clinical MSOT allows for non-invasive, label-free and real-time imaging of muscle oxygenation in health and disease with implications for diagnostics and therapy assessment in PAD. AME Publishing Company 2021-01 /pmc/articles/PMC7859778/ /pubmed/33553329 http://dx.doi.org/10.21037/atm-20-3321 Text en 2021 Annals of Translational Medicine. All rights reserved. https://creativecommons.org/licenses/by-nc-nd/4.0/Open Access Statement: This is an Open Access article distributed in accordance with the Creative Commons Attribution-NonCommercial-NoDerivs 4.0 International License (CC BY-NC-ND 4.0), which permits the non-commercial replication and distribution of the article with the strict proviso that no changes or edits are made and the original work is properly cited (including links to both the formal publication through the relevant DOI and the license). See: https://creativecommons.org/licenses/by-nc-nd/4.0 (https://creativecommons.org/licenses/by-nc-nd/4.0/) . |
spellingShingle | Original Article Karlas, Angelos Masthoff, Max Kallmayer, Michael Helfen, Anne Bariotakis, Michail Fasoula, Nikolina Alexia Schäfers, Michael Seidensticker, Max Eckstein, Hans-Henning Ntziachristos, Vasilis Wildgruber, Moritz Multispectral optoacoustic tomography of peripheral arterial disease based on muscle hemoglobin gradients—a pilot clinical study |
title | Multispectral optoacoustic tomography of peripheral arterial disease based on muscle hemoglobin gradients—a pilot clinical study |
title_full | Multispectral optoacoustic tomography of peripheral arterial disease based on muscle hemoglobin gradients—a pilot clinical study |
title_fullStr | Multispectral optoacoustic tomography of peripheral arterial disease based on muscle hemoglobin gradients—a pilot clinical study |
title_full_unstemmed | Multispectral optoacoustic tomography of peripheral arterial disease based on muscle hemoglobin gradients—a pilot clinical study |
title_short | Multispectral optoacoustic tomography of peripheral arterial disease based on muscle hemoglobin gradients—a pilot clinical study |
title_sort | multispectral optoacoustic tomography of peripheral arterial disease based on muscle hemoglobin gradients—a pilot clinical study |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7859778/ https://www.ncbi.nlm.nih.gov/pubmed/33553329 http://dx.doi.org/10.21037/atm-20-3321 |
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