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Characterization and In Vitro Evaluations of Injectable Calcium Phosphate Cement Doped with Magnesium and Strontium

[Image: see text] Injectable calcium phosphate cement is a promising biomaterial for hard tissue repair due to its osteoinductivity, biocompatibility properties, and its use to correct defect areas involving narrow cavities with limited accessibility by the minimally invasive technique. Microwave-sy...

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Autores principales: Arkin, Vetharaj HephzibahRajam, Narendrakumar, Uttamchand, Madhyastha, Harishkumar, Manjubala, Inderchand
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Chemical Society 2021
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7859950/
https://www.ncbi.nlm.nih.gov/pubmed/33553866
http://dx.doi.org/10.1021/acsomega.0c03927
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author Arkin, Vetharaj HephzibahRajam
Narendrakumar, Uttamchand
Madhyastha, Harishkumar
Manjubala, Inderchand
author_facet Arkin, Vetharaj HephzibahRajam
Narendrakumar, Uttamchand
Madhyastha, Harishkumar
Manjubala, Inderchand
author_sort Arkin, Vetharaj HephzibahRajam
collection PubMed
description [Image: see text] Injectable calcium phosphate cement is a promising biomaterial for hard tissue repair due to its osteoinductivity, biocompatibility properties, and its use to correct defect areas involving narrow cavities with limited accessibility by the minimally invasive technique. Microwave-synthesized hydroxyapatite (HA) was used for the preparation of cement. In recent years, both magnesium and strontium calcium phosphate cements have exhibited rapid setting, improved mechanical strength, and a good resorption rate. A big step still remains to develop injectable magnesium and strontium phosphate cements with ideal self-setting properties, adequate mechanical strength, and good biocompatibility for clinical applications. In this study, both magnesium and strontium were doped with synthesized semiamorphous and crystalline hydroxyapatite (HA). The powder mixture was mixed with Na(2)HPO(4), NaH(2)PO(4), and a carboxymethyl cellulose (CMC) solution to develop the novel magnesium and strontium calcium phosphate cement. The setting time, physiochemical properties of hardened cement, microstructure, mechanical strength, and injectability of the prepared cement were studied. The toxicity evaluation and cell adhesion, which are necessary to identify the suitability of the material for different applications, were quantified and investigated using fibroblast cells. The setting time of cement was reduced substantially for magnesium- or strontium-doped cement by 2 min. The phase composition of the hardened cement expresses the semiamorphous or crystalline phase of HA with additives. Smooth and complete injection of cement paste was observed in semiamorphous HA-based cement. The intercellular reactive oxygen stress (ROS) of the Sr(2+)-doped cement sample showed varied degrees of toxicity to cells in terms of different concentrations. The Mg(2+)-doped cement showed significant attachment of cells after treatment at varying incubation times.
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spelling pubmed-78599502021-02-05 Characterization and In Vitro Evaluations of Injectable Calcium Phosphate Cement Doped with Magnesium and Strontium Arkin, Vetharaj HephzibahRajam Narendrakumar, Uttamchand Madhyastha, Harishkumar Manjubala, Inderchand ACS Omega [Image: see text] Injectable calcium phosphate cement is a promising biomaterial for hard tissue repair due to its osteoinductivity, biocompatibility properties, and its use to correct defect areas involving narrow cavities with limited accessibility by the minimally invasive technique. Microwave-synthesized hydroxyapatite (HA) was used for the preparation of cement. In recent years, both magnesium and strontium calcium phosphate cements have exhibited rapid setting, improved mechanical strength, and a good resorption rate. A big step still remains to develop injectable magnesium and strontium phosphate cements with ideal self-setting properties, adequate mechanical strength, and good biocompatibility for clinical applications. In this study, both magnesium and strontium were doped with synthesized semiamorphous and crystalline hydroxyapatite (HA). The powder mixture was mixed with Na(2)HPO(4), NaH(2)PO(4), and a carboxymethyl cellulose (CMC) solution to develop the novel magnesium and strontium calcium phosphate cement. The setting time, physiochemical properties of hardened cement, microstructure, mechanical strength, and injectability of the prepared cement were studied. The toxicity evaluation and cell adhesion, which are necessary to identify the suitability of the material for different applications, were quantified and investigated using fibroblast cells. The setting time of cement was reduced substantially for magnesium- or strontium-doped cement by 2 min. The phase composition of the hardened cement expresses the semiamorphous or crystalline phase of HA with additives. Smooth and complete injection of cement paste was observed in semiamorphous HA-based cement. The intercellular reactive oxygen stress (ROS) of the Sr(2+)-doped cement sample showed varied degrees of toxicity to cells in terms of different concentrations. The Mg(2+)-doped cement showed significant attachment of cells after treatment at varying incubation times. American Chemical Society 2021-01-20 /pmc/articles/PMC7859950/ /pubmed/33553866 http://dx.doi.org/10.1021/acsomega.0c03927 Text en © 2021 The Authors. Published by American Chemical Society This is an open access article published under a Creative Commons Non-Commercial No Derivative Works (CC-BY-NC-ND) Attribution License (http://pubs.acs.org/page/policy/authorchoice_ccbyncnd_termsofuse.html) , which permits copying and redistribution of the article, and creation of adaptations, all for non-commercial purposes.
spellingShingle Arkin, Vetharaj HephzibahRajam
Narendrakumar, Uttamchand
Madhyastha, Harishkumar
Manjubala, Inderchand
Characterization and In Vitro Evaluations of Injectable Calcium Phosphate Cement Doped with Magnesium and Strontium
title Characterization and In Vitro Evaluations of Injectable Calcium Phosphate Cement Doped with Magnesium and Strontium
title_full Characterization and In Vitro Evaluations of Injectable Calcium Phosphate Cement Doped with Magnesium and Strontium
title_fullStr Characterization and In Vitro Evaluations of Injectable Calcium Phosphate Cement Doped with Magnesium and Strontium
title_full_unstemmed Characterization and In Vitro Evaluations of Injectable Calcium Phosphate Cement Doped with Magnesium and Strontium
title_short Characterization and In Vitro Evaluations of Injectable Calcium Phosphate Cement Doped with Magnesium and Strontium
title_sort characterization and in vitro evaluations of injectable calcium phosphate cement doped with magnesium and strontium
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7859950/
https://www.ncbi.nlm.nih.gov/pubmed/33553866
http://dx.doi.org/10.1021/acsomega.0c03927
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