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The Traditional Chinese Medicine Fuyou Formula Alleviates Precocious Puberty by Inhibiting GPR54/GnRH in the Hypothalamus

The purpose of this study was to explore the effect of the traditional Chinese medicine Fuyou formula on precocious puberty (PP). The Fy formula may exert an effect in female rats with PP and GT-7 cells through the GPR54/GnRH signaling pathway. To confirm the effect of the Fy formula on PP through t...

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Autores principales: Bai, Guo-liang, Hu, Kai-li, Huan, Yi, Wang, Xing, Lei, Lei, Zhang, Meng, Guo, Chun-yan, Chang, Hong-sheng, Zhao, Li-bo, Liu, Jing, Shen, Zhu-fang, Wang, Xiao-ling, Ni, Xin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7859969/
https://www.ncbi.nlm.nih.gov/pubmed/33551803
http://dx.doi.org/10.3389/fphar.2020.596525
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author Bai, Guo-liang
Hu, Kai-li
Huan, Yi
Wang, Xing
Lei, Lei
Zhang, Meng
Guo, Chun-yan
Chang, Hong-sheng
Zhao, Li-bo
Liu, Jing
Shen, Zhu-fang
Wang, Xiao-ling
Ni, Xin
author_facet Bai, Guo-liang
Hu, Kai-li
Huan, Yi
Wang, Xing
Lei, Lei
Zhang, Meng
Guo, Chun-yan
Chang, Hong-sheng
Zhao, Li-bo
Liu, Jing
Shen, Zhu-fang
Wang, Xiao-ling
Ni, Xin
author_sort Bai, Guo-liang
collection PubMed
description The purpose of this study was to explore the effect of the traditional Chinese medicine Fuyou formula on precocious puberty (PP). The Fy formula may exert an effect in female rats with PP and GT-7 cells through the GPR54/GnRH signaling pathway. To confirm the effect of the Fy formula on PP through the GPR54/GnRH signaling pathway, we first treated GT1-7 cells with the Fy formula and observed changes in the expression of related genes and proteins and in GnRH secretion. Then, we randomly divided young female Sprague-Dawley rats into the control group, model group, leuprorelin group and the Fy formula group. A PP model was established by injection of danazol on postnatal day 5, and the Fy formula was administered on PND15. The time of vaginal opening, the wet weights of the ovary and uterus, serum hormone levels and the expression of hypothalamic-related genes were observed. We found that the Fy formula delayed vaginal opening, decreased the wet weights and coefficients of the ovary and uterus, decreased the levels of serum hormones (E2, follicle-stimulating hormone and luteinizing hormone) and the cellular GnRH level, and downregulated the gene expression of Kiss1, GPR54 and GnRH in the hypothalamus and the gene and protein expression of GPR54 and GnRH in GT1-7 cells. In conclusion, the Fy formula may alleviate PP via the GPR54/GnRH signaling pathway.
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spelling pubmed-78599692021-02-05 The Traditional Chinese Medicine Fuyou Formula Alleviates Precocious Puberty by Inhibiting GPR54/GnRH in the Hypothalamus Bai, Guo-liang Hu, Kai-li Huan, Yi Wang, Xing Lei, Lei Zhang, Meng Guo, Chun-yan Chang, Hong-sheng Zhao, Li-bo Liu, Jing Shen, Zhu-fang Wang, Xiao-ling Ni, Xin Front Pharmacol Pharmacology The purpose of this study was to explore the effect of the traditional Chinese medicine Fuyou formula on precocious puberty (PP). The Fy formula may exert an effect in female rats with PP and GT-7 cells through the GPR54/GnRH signaling pathway. To confirm the effect of the Fy formula on PP through the GPR54/GnRH signaling pathway, we first treated GT1-7 cells with the Fy formula and observed changes in the expression of related genes and proteins and in GnRH secretion. Then, we randomly divided young female Sprague-Dawley rats into the control group, model group, leuprorelin group and the Fy formula group. A PP model was established by injection of danazol on postnatal day 5, and the Fy formula was administered on PND15. The time of vaginal opening, the wet weights of the ovary and uterus, serum hormone levels and the expression of hypothalamic-related genes were observed. We found that the Fy formula delayed vaginal opening, decreased the wet weights and coefficients of the ovary and uterus, decreased the levels of serum hormones (E2, follicle-stimulating hormone and luteinizing hormone) and the cellular GnRH level, and downregulated the gene expression of Kiss1, GPR54 and GnRH in the hypothalamus and the gene and protein expression of GPR54 and GnRH in GT1-7 cells. In conclusion, the Fy formula may alleviate PP via the GPR54/GnRH signaling pathway. Frontiers Media S.A. 2021-01-21 /pmc/articles/PMC7859969/ /pubmed/33551803 http://dx.doi.org/10.3389/fphar.2020.596525 Text en Copyright © 2021 Bai, Hu, Huan, Wang, Lei, Zhang, Guo, Chang, Zhao, Liu, Shen, Wang and Ni. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Pharmacology
Bai, Guo-liang
Hu, Kai-li
Huan, Yi
Wang, Xing
Lei, Lei
Zhang, Meng
Guo, Chun-yan
Chang, Hong-sheng
Zhao, Li-bo
Liu, Jing
Shen, Zhu-fang
Wang, Xiao-ling
Ni, Xin
The Traditional Chinese Medicine Fuyou Formula Alleviates Precocious Puberty by Inhibiting GPR54/GnRH in the Hypothalamus
title The Traditional Chinese Medicine Fuyou Formula Alleviates Precocious Puberty by Inhibiting GPR54/GnRH in the Hypothalamus
title_full The Traditional Chinese Medicine Fuyou Formula Alleviates Precocious Puberty by Inhibiting GPR54/GnRH in the Hypothalamus
title_fullStr The Traditional Chinese Medicine Fuyou Formula Alleviates Precocious Puberty by Inhibiting GPR54/GnRH in the Hypothalamus
title_full_unstemmed The Traditional Chinese Medicine Fuyou Formula Alleviates Precocious Puberty by Inhibiting GPR54/GnRH in the Hypothalamus
title_short The Traditional Chinese Medicine Fuyou Formula Alleviates Precocious Puberty by Inhibiting GPR54/GnRH in the Hypothalamus
title_sort traditional chinese medicine fuyou formula alleviates precocious puberty by inhibiting gpr54/gnrh in the hypothalamus
topic Pharmacology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7859969/
https://www.ncbi.nlm.nih.gov/pubmed/33551803
http://dx.doi.org/10.3389/fphar.2020.596525
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