LncRNA LINC01116 sponges miR-93-5p to promote cell invasion and migration in small cell lung cancer

BACKGROUND: LINC01116 is a recently identified oncogenic lncRNA in glioma. Differential expression analysis using the public gene expression analysis tool GEPIA revealed the upregulation of LINC01116 in lung cancer. We studied the functions of LINC01116 in small cell lung cancer (SCLC). METHODS: The...

Descripción completa

Detalles Bibliográficos
Autores principales: Liu, Wenzhou, Liang, Feihai, Yang, Guangyu, Xian, Lei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2021
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7860012/
https://www.ncbi.nlm.nih.gov/pubmed/33535997
http://dx.doi.org/10.1186/s12890-020-01369-3
_version_ 1783646853759238144
author Liu, Wenzhou
Liang, Feihai
Yang, Guangyu
Xian, Lei
author_facet Liu, Wenzhou
Liang, Feihai
Yang, Guangyu
Xian, Lei
author_sort Liu, Wenzhou
collection PubMed
description BACKGROUND: LINC01116 is a recently identified oncogenic lncRNA in glioma. Differential expression analysis using the public gene expression analysis tool GEPIA revealed the upregulation of LINC01116 in lung cancer. We studied the functions of LINC01116 in small cell lung cancer (SCLC). METHODS: The expression of LINC01116 in several types of cancer tissue and the paired non-tumor tissues was evaluated by GEPIA. The effects of the overexpression of LINC01116 and miR-93-5p on the expression of STAT3 were evaluated. The effects of the overexpression of LINC01116, miR-93-5p and STAT3 on SHP-77 cell behaviors were evaluated by Transwell assays. RESULTS: LINC01116 was highly expressed in SCLC and predicted poor survival. In SCLC tissues, the expression of LINC01116 was positively correlated with STAT3. Bioinformatics analysis revealed that miR-93-5p may target LINC01116. Overexpression of LINC01116 increased STAT3 but did not affect the expression of miR-93-5p. Transwell assay showed that LINC01116 and STAT3 increased cell invasion and migration rates. MiR-93-5p played an suppressed cell behaviors and suppressed the role of LINC01116. CONCLUSION: Therefore, LINC01116 might upregulate STA3 by sponging miR-93-5p, thereby promoting cell invasion and migration in SCLC.
format Online
Article
Text
id pubmed-7860012
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher BioMed Central
record_format MEDLINE/PubMed
spelling pubmed-78600122021-02-04 LncRNA LINC01116 sponges miR-93-5p to promote cell invasion and migration in small cell lung cancer Liu, Wenzhou Liang, Feihai Yang, Guangyu Xian, Lei BMC Pulm Med Research Article BACKGROUND: LINC01116 is a recently identified oncogenic lncRNA in glioma. Differential expression analysis using the public gene expression analysis tool GEPIA revealed the upregulation of LINC01116 in lung cancer. We studied the functions of LINC01116 in small cell lung cancer (SCLC). METHODS: The expression of LINC01116 in several types of cancer tissue and the paired non-tumor tissues was evaluated by GEPIA. The effects of the overexpression of LINC01116 and miR-93-5p on the expression of STAT3 were evaluated. The effects of the overexpression of LINC01116, miR-93-5p and STAT3 on SHP-77 cell behaviors were evaluated by Transwell assays. RESULTS: LINC01116 was highly expressed in SCLC and predicted poor survival. In SCLC tissues, the expression of LINC01116 was positively correlated with STAT3. Bioinformatics analysis revealed that miR-93-5p may target LINC01116. Overexpression of LINC01116 increased STAT3 but did not affect the expression of miR-93-5p. Transwell assay showed that LINC01116 and STAT3 increased cell invasion and migration rates. MiR-93-5p played an suppressed cell behaviors and suppressed the role of LINC01116. CONCLUSION: Therefore, LINC01116 might upregulate STA3 by sponging miR-93-5p, thereby promoting cell invasion and migration in SCLC. BioMed Central 2021-02-03 /pmc/articles/PMC7860012/ /pubmed/33535997 http://dx.doi.org/10.1186/s12890-020-01369-3 Text en © The Author(s) 2021 Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Research Article
Liu, Wenzhou
Liang, Feihai
Yang, Guangyu
Xian, Lei
LncRNA LINC01116 sponges miR-93-5p to promote cell invasion and migration in small cell lung cancer
title LncRNA LINC01116 sponges miR-93-5p to promote cell invasion and migration in small cell lung cancer
title_full LncRNA LINC01116 sponges miR-93-5p to promote cell invasion and migration in small cell lung cancer
title_fullStr LncRNA LINC01116 sponges miR-93-5p to promote cell invasion and migration in small cell lung cancer
title_full_unstemmed LncRNA LINC01116 sponges miR-93-5p to promote cell invasion and migration in small cell lung cancer
title_short LncRNA LINC01116 sponges miR-93-5p to promote cell invasion and migration in small cell lung cancer
title_sort lncrna linc01116 sponges mir-93-5p to promote cell invasion and migration in small cell lung cancer
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7860012/
https://www.ncbi.nlm.nih.gov/pubmed/33535997
http://dx.doi.org/10.1186/s12890-020-01369-3
work_keys_str_mv AT liuwenzhou lncrnalinc01116spongesmir935ptopromotecellinvasionandmigrationinsmallcelllungcancer
AT liangfeihai lncrnalinc01116spongesmir935ptopromotecellinvasionandmigrationinsmallcelllungcancer
AT yangguangyu lncrnalinc01116spongesmir935ptopromotecellinvasionandmigrationinsmallcelllungcancer
AT xianlei lncrnalinc01116spongesmir935ptopromotecellinvasionandmigrationinsmallcelllungcancer

Ejemplares similares