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The long non-coding RNA GHSROS reprograms prostate cancer cell lines toward a more aggressive phenotype

It is now appreciated that long non-coding RNAs (lncRNAs) are important players in orchestrating cancer progression. In this study we characterized GHSROS, a human lncRNA gene on the opposite DNA strand (antisense) to the ghrelin receptor gene, in prostate cancer. The lncRNA was upregulated by prost...

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Autores principales: Thomas, Patrick B., Jeffery, Penny, Gahete, Manuel D., Whiteside, Eliza, Walpole, Carina, Maugham, Michelle, Jovanovic, Lidija, Gunter, Jennifer, Williams, Elizabeth, Nelson, Colleen, Herington, Adrian, Luque, Raul M., Veedu, Rakesh, Chopin, Lisa K., Seim, Inge
Formato: Online Artículo Texto
Lenguaje:English
Publicado: PeerJ Inc. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7860111/
https://www.ncbi.nlm.nih.gov/pubmed/33585078
http://dx.doi.org/10.7717/peerj.10280
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author Thomas, Patrick B.
Jeffery, Penny
Gahete, Manuel D.
Whiteside, Eliza
Walpole, Carina
Maugham, Michelle
Jovanovic, Lidija
Gunter, Jennifer
Williams, Elizabeth
Nelson, Colleen
Herington, Adrian
Luque, Raul M.
Veedu, Rakesh
Chopin, Lisa K.
Seim, Inge
author_facet Thomas, Patrick B.
Jeffery, Penny
Gahete, Manuel D.
Whiteside, Eliza
Walpole, Carina
Maugham, Michelle
Jovanovic, Lidija
Gunter, Jennifer
Williams, Elizabeth
Nelson, Colleen
Herington, Adrian
Luque, Raul M.
Veedu, Rakesh
Chopin, Lisa K.
Seim, Inge
author_sort Thomas, Patrick B.
collection PubMed
description It is now appreciated that long non-coding RNAs (lncRNAs) are important players in orchestrating cancer progression. In this study we characterized GHSROS, a human lncRNA gene on the opposite DNA strand (antisense) to the ghrelin receptor gene, in prostate cancer. The lncRNA was upregulated by prostate tumors from different clinical datasets. Transcriptome data revealed that GHSROS alters the expression of cancer-associated genes. Functional analyses in vitro showed that GHSROS mediates tumor growth, migration and survival, and resistance to the cytotoxic drug docetaxel. Increased cellular proliferation of GHSROS-overexpressing PC3, DU145, and LNCaP prostate cancer cell lines in vitro was recapitulated in a subcutaneous xenograft model. Conversely, in vitro antisense oligonucleotide inhibition of the lncRNA reciprocally regulated cell growth and migration, and gene expression. Notably, GHSROS modulates the expression of PPP2R2C, the loss of which may drive androgen receptor pathway-independent prostate tumor progression in a subset of prostate cancers. Collectively, our findings suggest that GHSROS can reprogram prostate cancer cells toward a more aggressive phenotype and that this lncRNA may represent a potential therapeutic target.
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spelling pubmed-78601112021-02-12 The long non-coding RNA GHSROS reprograms prostate cancer cell lines toward a more aggressive phenotype Thomas, Patrick B. Jeffery, Penny Gahete, Manuel D. Whiteside, Eliza Walpole, Carina Maugham, Michelle Jovanovic, Lidija Gunter, Jennifer Williams, Elizabeth Nelson, Colleen Herington, Adrian Luque, Raul M. Veedu, Rakesh Chopin, Lisa K. Seim, Inge PeerJ Cell Biology It is now appreciated that long non-coding RNAs (lncRNAs) are important players in orchestrating cancer progression. In this study we characterized GHSROS, a human lncRNA gene on the opposite DNA strand (antisense) to the ghrelin receptor gene, in prostate cancer. The lncRNA was upregulated by prostate tumors from different clinical datasets. Transcriptome data revealed that GHSROS alters the expression of cancer-associated genes. Functional analyses in vitro showed that GHSROS mediates tumor growth, migration and survival, and resistance to the cytotoxic drug docetaxel. Increased cellular proliferation of GHSROS-overexpressing PC3, DU145, and LNCaP prostate cancer cell lines in vitro was recapitulated in a subcutaneous xenograft model. Conversely, in vitro antisense oligonucleotide inhibition of the lncRNA reciprocally regulated cell growth and migration, and gene expression. Notably, GHSROS modulates the expression of PPP2R2C, the loss of which may drive androgen receptor pathway-independent prostate tumor progression in a subset of prostate cancers. Collectively, our findings suggest that GHSROS can reprogram prostate cancer cells toward a more aggressive phenotype and that this lncRNA may represent a potential therapeutic target. PeerJ Inc. 2021-02-01 /pmc/articles/PMC7860111/ /pubmed/33585078 http://dx.doi.org/10.7717/peerj.10280 Text en ©2021 Thomas et al. https://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, reproduction and adaptation in any medium and for any purpose provided that it is properly attributed. For attribution, the original author(s), title, publication source (PeerJ) and either DOI or URL of the article must be cited.
spellingShingle Cell Biology
Thomas, Patrick B.
Jeffery, Penny
Gahete, Manuel D.
Whiteside, Eliza
Walpole, Carina
Maugham, Michelle
Jovanovic, Lidija
Gunter, Jennifer
Williams, Elizabeth
Nelson, Colleen
Herington, Adrian
Luque, Raul M.
Veedu, Rakesh
Chopin, Lisa K.
Seim, Inge
The long non-coding RNA GHSROS reprograms prostate cancer cell lines toward a more aggressive phenotype
title The long non-coding RNA GHSROS reprograms prostate cancer cell lines toward a more aggressive phenotype
title_full The long non-coding RNA GHSROS reprograms prostate cancer cell lines toward a more aggressive phenotype
title_fullStr The long non-coding RNA GHSROS reprograms prostate cancer cell lines toward a more aggressive phenotype
title_full_unstemmed The long non-coding RNA GHSROS reprograms prostate cancer cell lines toward a more aggressive phenotype
title_short The long non-coding RNA GHSROS reprograms prostate cancer cell lines toward a more aggressive phenotype
title_sort long non-coding rna ghsros reprograms prostate cancer cell lines toward a more aggressive phenotype
topic Cell Biology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7860111/
https://www.ncbi.nlm.nih.gov/pubmed/33585078
http://dx.doi.org/10.7717/peerj.10280
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