DOCK11 and DENND2A play pivotal roles in the maintenance of hepatitis B virus in host cells

Human hepatitis B virus (HBV) infection remains a serious health problem worldwide. However, the mechanism for the maintenance of HBV in a latent state within host cells remains unclear. Here, using single-cell RNA sequencing analysis, we identified four genes linked to the maintenance of HBV in a l...

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Detalles Bibliográficos
Autores principales: Hashimoto, Shinichi, Shirasaki, Takayoshi, Yamashita, Taro, Iwabuchi, Sadahiro, Suzuki, Yutaka, Takamura, Yuzuru, Ukita, Yoshiaki, Deshimaru, Shungo, Okayama, Toshitugu, Ikeo, Kazuho, Kuroki, Kazuyuki, Kawaguchi, Kazunori, Mizukoshi, Eishiro, Matsushima, Kouji, Honda, Masao, Kaneko, Shuichi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2021
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7861363/
https://www.ncbi.nlm.nih.gov/pubmed/33539396
http://dx.doi.org/10.1371/journal.pone.0246313
Descripción
Sumario:Human hepatitis B virus (HBV) infection remains a serious health problem worldwide. However, the mechanism for the maintenance of HBV in a latent state within host cells remains unclear. Here, using single-cell RNA sequencing analysis, we identified four genes linked to the maintenance of HBV in a liver cell line expressing HBV RNA at a low frequency. These genes included DOCK11 and DENND2A, which encode small GTPase regulators. In primary human hepatocytes infected with HBV, knockdown of these two genes decreased the amount of both HBV DNA and covalently closed circular DNA to below the limit of detection. Our findings reveal a role for DOCK11 and DENND2A in the maintenance of HBV.

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