DOCK11 and DENND2A play pivotal roles in the maintenance of hepatitis B virus in host cells

Human hepatitis B virus (HBV) infection remains a serious health problem worldwide. However, the mechanism for the maintenance of HBV in a latent state within host cells remains unclear. Here, using single-cell RNA sequencing analysis, we identified four genes linked to the maintenance of HBV in a l...

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Autores principales: Hashimoto, Shinichi, Shirasaki, Takayoshi, Yamashita, Taro, Iwabuchi, Sadahiro, Suzuki, Yutaka, Takamura, Yuzuru, Ukita, Yoshiaki, Deshimaru, Shungo, Okayama, Toshitugu, Ikeo, Kazuho, Kuroki, Kazuyuki, Kawaguchi, Kazunori, Mizukoshi, Eishiro, Matsushima, Kouji, Honda, Masao, Kaneko, Shuichi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2021
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7861363/
https://www.ncbi.nlm.nih.gov/pubmed/33539396
http://dx.doi.org/10.1371/journal.pone.0246313
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author Hashimoto, Shinichi
Shirasaki, Takayoshi
Yamashita, Taro
Iwabuchi, Sadahiro
Suzuki, Yutaka
Takamura, Yuzuru
Ukita, Yoshiaki
Deshimaru, Shungo
Okayama, Toshitugu
Ikeo, Kazuho
Kuroki, Kazuyuki
Kawaguchi, Kazunori
Mizukoshi, Eishiro
Matsushima, Kouji
Honda, Masao
Kaneko, Shuichi
author_facet Hashimoto, Shinichi
Shirasaki, Takayoshi
Yamashita, Taro
Iwabuchi, Sadahiro
Suzuki, Yutaka
Takamura, Yuzuru
Ukita, Yoshiaki
Deshimaru, Shungo
Okayama, Toshitugu
Ikeo, Kazuho
Kuroki, Kazuyuki
Kawaguchi, Kazunori
Mizukoshi, Eishiro
Matsushima, Kouji
Honda, Masao
Kaneko, Shuichi
author_sort Hashimoto, Shinichi
collection PubMed
description Human hepatitis B virus (HBV) infection remains a serious health problem worldwide. However, the mechanism for the maintenance of HBV in a latent state within host cells remains unclear. Here, using single-cell RNA sequencing analysis, we identified four genes linked to the maintenance of HBV in a liver cell line expressing HBV RNA at a low frequency. These genes included DOCK11 and DENND2A, which encode small GTPase regulators. In primary human hepatocytes infected with HBV, knockdown of these two genes decreased the amount of both HBV DNA and covalently closed circular DNA to below the limit of detection. Our findings reveal a role for DOCK11 and DENND2A in the maintenance of HBV.
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spelling pubmed-78613632021-02-12 DOCK11 and DENND2A play pivotal roles in the maintenance of hepatitis B virus in host cells Hashimoto, Shinichi Shirasaki, Takayoshi Yamashita, Taro Iwabuchi, Sadahiro Suzuki, Yutaka Takamura, Yuzuru Ukita, Yoshiaki Deshimaru, Shungo Okayama, Toshitugu Ikeo, Kazuho Kuroki, Kazuyuki Kawaguchi, Kazunori Mizukoshi, Eishiro Matsushima, Kouji Honda, Masao Kaneko, Shuichi PLoS One Research Article Human hepatitis B virus (HBV) infection remains a serious health problem worldwide. However, the mechanism for the maintenance of HBV in a latent state within host cells remains unclear. Here, using single-cell RNA sequencing analysis, we identified four genes linked to the maintenance of HBV in a liver cell line expressing HBV RNA at a low frequency. These genes included DOCK11 and DENND2A, which encode small GTPase regulators. In primary human hepatocytes infected with HBV, knockdown of these two genes decreased the amount of both HBV DNA and covalently closed circular DNA to below the limit of detection. Our findings reveal a role for DOCK11 and DENND2A in the maintenance of HBV. Public Library of Science 2021-02-04 /pmc/articles/PMC7861363/ /pubmed/33539396 http://dx.doi.org/10.1371/journal.pone.0246313 Text en © 2021 Hashimoto et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Hashimoto, Shinichi
Shirasaki, Takayoshi
Yamashita, Taro
Iwabuchi, Sadahiro
Suzuki, Yutaka
Takamura, Yuzuru
Ukita, Yoshiaki
Deshimaru, Shungo
Okayama, Toshitugu
Ikeo, Kazuho
Kuroki, Kazuyuki
Kawaguchi, Kazunori
Mizukoshi, Eishiro
Matsushima, Kouji
Honda, Masao
Kaneko, Shuichi
DOCK11 and DENND2A play pivotal roles in the maintenance of hepatitis B virus in host cells
title DOCK11 and DENND2A play pivotal roles in the maintenance of hepatitis B virus in host cells
title_full DOCK11 and DENND2A play pivotal roles in the maintenance of hepatitis B virus in host cells
title_fullStr DOCK11 and DENND2A play pivotal roles in the maintenance of hepatitis B virus in host cells
title_full_unstemmed DOCK11 and DENND2A play pivotal roles in the maintenance of hepatitis B virus in host cells
title_short DOCK11 and DENND2A play pivotal roles in the maintenance of hepatitis B virus in host cells
title_sort dock11 and dennd2a play pivotal roles in the maintenance of hepatitis b virus in host cells
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7861363/
https://www.ncbi.nlm.nih.gov/pubmed/33539396
http://dx.doi.org/10.1371/journal.pone.0246313
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