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Novel three-dimensional biochip pulmonary sarcoidosis model
Sarcoidosis is a multi-system disorder of granulomatous inflammation which most commonly affects the lungs. Its etiology and pathogenesis are not well defined in part due to the lack of reliable modeling. Here, we present the development of an in vitro three-dimensional lung-on-chip biochip designed...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7861546/ https://www.ncbi.nlm.nih.gov/pubmed/33539409 http://dx.doi.org/10.1371/journal.pone.0245805 |
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author | Calcagno, Tess M. Zhang, Chongxu Tian, Runxia Ebrahimi, Babak Mirsaeidi, Mehdi |
author_facet | Calcagno, Tess M. Zhang, Chongxu Tian, Runxia Ebrahimi, Babak Mirsaeidi, Mehdi |
author_sort | Calcagno, Tess M. |
collection | PubMed |
description | Sarcoidosis is a multi-system disorder of granulomatous inflammation which most commonly affects the lungs. Its etiology and pathogenesis are not well defined in part due to the lack of reliable modeling. Here, we present the development of an in vitro three-dimensional lung-on-chip biochip designed to mimic granuloma formation. A lung on chip fluidic macrodevice was developed and added to our previously developed a lung-on-membrane model (LOMM). Granulomas were cultured from blood samples of patients with sarcoidosis and then inserted in the air-lung-interface of the microchip to create a three-dimensional biochip pulmonary sarcoidosis model (3D BSGM). Cytokines were measured after 48 hours. ELISA testing was performed to measure cytokine response difference between LOMM with 3D BSGM. There were statistically significant differences in IL-1ß (P = 0.001953), IL-6 (P = 0.001953), GM-CSF (P = 0.001953), and INF-γ expressions (P = 0.09375) between two groups. The current model represents the first 3D biochip sarcoidosis model created by adding a microfluidics system to a dual-chambered lung on membrane model and introducing developed sarcoid-granuloma to its air-lung-interface. |
format | Online Article Text |
id | pubmed-7861546 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-78615462021-02-12 Novel three-dimensional biochip pulmonary sarcoidosis model Calcagno, Tess M. Zhang, Chongxu Tian, Runxia Ebrahimi, Babak Mirsaeidi, Mehdi PLoS One Research Article Sarcoidosis is a multi-system disorder of granulomatous inflammation which most commonly affects the lungs. Its etiology and pathogenesis are not well defined in part due to the lack of reliable modeling. Here, we present the development of an in vitro three-dimensional lung-on-chip biochip designed to mimic granuloma formation. A lung on chip fluidic macrodevice was developed and added to our previously developed a lung-on-membrane model (LOMM). Granulomas were cultured from blood samples of patients with sarcoidosis and then inserted in the air-lung-interface of the microchip to create a three-dimensional biochip pulmonary sarcoidosis model (3D BSGM). Cytokines were measured after 48 hours. ELISA testing was performed to measure cytokine response difference between LOMM with 3D BSGM. There were statistically significant differences in IL-1ß (P = 0.001953), IL-6 (P = 0.001953), GM-CSF (P = 0.001953), and INF-γ expressions (P = 0.09375) between two groups. The current model represents the first 3D biochip sarcoidosis model created by adding a microfluidics system to a dual-chambered lung on membrane model and introducing developed sarcoid-granuloma to its air-lung-interface. Public Library of Science 2021-02-04 /pmc/articles/PMC7861546/ /pubmed/33539409 http://dx.doi.org/10.1371/journal.pone.0245805 Text en © 2021 Calcagno et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Calcagno, Tess M. Zhang, Chongxu Tian, Runxia Ebrahimi, Babak Mirsaeidi, Mehdi Novel three-dimensional biochip pulmonary sarcoidosis model |
title | Novel three-dimensional biochip pulmonary sarcoidosis model |
title_full | Novel three-dimensional biochip pulmonary sarcoidosis model |
title_fullStr | Novel three-dimensional biochip pulmonary sarcoidosis model |
title_full_unstemmed | Novel three-dimensional biochip pulmonary sarcoidosis model |
title_short | Novel three-dimensional biochip pulmonary sarcoidosis model |
title_sort | novel three-dimensional biochip pulmonary sarcoidosis model |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7861546/ https://www.ncbi.nlm.nih.gov/pubmed/33539409 http://dx.doi.org/10.1371/journal.pone.0245805 |
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