Cargando…

Novel three-dimensional biochip pulmonary sarcoidosis model

Sarcoidosis is a multi-system disorder of granulomatous inflammation which most commonly affects the lungs. Its etiology and pathogenesis are not well defined in part due to the lack of reliable modeling. Here, we present the development of an in vitro three-dimensional lung-on-chip biochip designed...

Descripción completa

Detalles Bibliográficos
Autores principales: Calcagno, Tess M., Zhang, Chongxu, Tian, Runxia, Ebrahimi, Babak, Mirsaeidi, Mehdi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7861546/
https://www.ncbi.nlm.nih.gov/pubmed/33539409
http://dx.doi.org/10.1371/journal.pone.0245805
_version_ 1783647106187132928
author Calcagno, Tess M.
Zhang, Chongxu
Tian, Runxia
Ebrahimi, Babak
Mirsaeidi, Mehdi
author_facet Calcagno, Tess M.
Zhang, Chongxu
Tian, Runxia
Ebrahimi, Babak
Mirsaeidi, Mehdi
author_sort Calcagno, Tess M.
collection PubMed
description Sarcoidosis is a multi-system disorder of granulomatous inflammation which most commonly affects the lungs. Its etiology and pathogenesis are not well defined in part due to the lack of reliable modeling. Here, we present the development of an in vitro three-dimensional lung-on-chip biochip designed to mimic granuloma formation. A lung on chip fluidic macrodevice was developed and added to our previously developed a lung-on-membrane model (LOMM). Granulomas were cultured from blood samples of patients with sarcoidosis and then inserted in the air-lung-interface of the microchip to create a three-dimensional biochip pulmonary sarcoidosis model (3D BSGM). Cytokines were measured after 48 hours. ELISA testing was performed to measure cytokine response difference between LOMM with 3D BSGM. There were statistically significant differences in IL-1ß (P = 0.001953), IL-6 (P = 0.001953), GM-CSF (P = 0.001953), and INF-γ expressions (P = 0.09375) between two groups. The current model represents the first 3D biochip sarcoidosis model created by adding a microfluidics system to a dual-chambered lung on membrane model and introducing developed sarcoid-granuloma to its air-lung-interface.
format Online
Article
Text
id pubmed-7861546
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher Public Library of Science
record_format MEDLINE/PubMed
spelling pubmed-78615462021-02-12 Novel three-dimensional biochip pulmonary sarcoidosis model Calcagno, Tess M. Zhang, Chongxu Tian, Runxia Ebrahimi, Babak Mirsaeidi, Mehdi PLoS One Research Article Sarcoidosis is a multi-system disorder of granulomatous inflammation which most commonly affects the lungs. Its etiology and pathogenesis are not well defined in part due to the lack of reliable modeling. Here, we present the development of an in vitro three-dimensional lung-on-chip biochip designed to mimic granuloma formation. A lung on chip fluidic macrodevice was developed and added to our previously developed a lung-on-membrane model (LOMM). Granulomas were cultured from blood samples of patients with sarcoidosis and then inserted in the air-lung-interface of the microchip to create a three-dimensional biochip pulmonary sarcoidosis model (3D BSGM). Cytokines were measured after 48 hours. ELISA testing was performed to measure cytokine response difference between LOMM with 3D BSGM. There were statistically significant differences in IL-1ß (P = 0.001953), IL-6 (P = 0.001953), GM-CSF (P = 0.001953), and INF-γ expressions (P = 0.09375) between two groups. The current model represents the first 3D biochip sarcoidosis model created by adding a microfluidics system to a dual-chambered lung on membrane model and introducing developed sarcoid-granuloma to its air-lung-interface. Public Library of Science 2021-02-04 /pmc/articles/PMC7861546/ /pubmed/33539409 http://dx.doi.org/10.1371/journal.pone.0245805 Text en © 2021 Calcagno et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Calcagno, Tess M.
Zhang, Chongxu
Tian, Runxia
Ebrahimi, Babak
Mirsaeidi, Mehdi
Novel three-dimensional biochip pulmonary sarcoidosis model
title Novel three-dimensional biochip pulmonary sarcoidosis model
title_full Novel three-dimensional biochip pulmonary sarcoidosis model
title_fullStr Novel three-dimensional biochip pulmonary sarcoidosis model
title_full_unstemmed Novel three-dimensional biochip pulmonary sarcoidosis model
title_short Novel three-dimensional biochip pulmonary sarcoidosis model
title_sort novel three-dimensional biochip pulmonary sarcoidosis model
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7861546/
https://www.ncbi.nlm.nih.gov/pubmed/33539409
http://dx.doi.org/10.1371/journal.pone.0245805
work_keys_str_mv AT calcagnotessm novelthreedimensionalbiochippulmonarysarcoidosismodel
AT zhangchongxu novelthreedimensionalbiochippulmonarysarcoidosismodel
AT tianrunxia novelthreedimensionalbiochippulmonarysarcoidosismodel
AT ebrahimibabak novelthreedimensionalbiochippulmonarysarcoidosismodel
AT mirsaeidimehdi novelthreedimensionalbiochippulmonarysarcoidosismodel