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The enhancer activity of long interspersed nuclear element derived microRNA 625 induced by NF-κB

Transposable elements (TEs) are DNA sequences that cut or introduced into the genome, and they represent a massive portion of the human genome. TEs generate a considerable number of microRNAs (miRNAs) are derived from TEs (MDTEs). Numerous miRNAs are related to cancer, and hsa-miRNA-625 is a well-kn...

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Autores principales: Lee, Hee-Eun, Park, Sang-Je, Huh, Jae-Won, Imai, Hiroo, Kim, Heui-Soo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7862687/
https://www.ncbi.nlm.nih.gov/pubmed/33542430
http://dx.doi.org/10.1038/s41598-021-82735-x
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author Lee, Hee-Eun
Park, Sang-Je
Huh, Jae-Won
Imai, Hiroo
Kim, Heui-Soo
author_facet Lee, Hee-Eun
Park, Sang-Je
Huh, Jae-Won
Imai, Hiroo
Kim, Heui-Soo
author_sort Lee, Hee-Eun
collection PubMed
description Transposable elements (TEs) are DNA sequences that cut or introduced into the genome, and they represent a massive portion of the human genome. TEs generate a considerable number of microRNAs (miRNAs) are derived from TEs (MDTEs). Numerous miRNAs are related to cancer, and hsa-miRNA-625 is a well-known oncomiR derived from long interspersed nuclear elements (LINEs). The relative expression of hsa-miRNA-625-5p differs in humans, chimpanzees, crab-eating monkeys, and mice, and four primers were designed against the 3′UTR of GATAD2B to analyze the different quantities of canonical binding sites and the location of miRNA binding sites. Luciferase assay was performed to score for the interaction between hsa-miRNA-625 and the 3′UTR of GATAD2B, while blocking NF-κB. In summary, the different numbers of canonical binding sites and the locations of miRNA binding sites affect gene expression, and NF-κB induces the enhancer activity of hsa-miRNA-625-5p by sharing the binding sites.
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spelling pubmed-78626872021-02-08 The enhancer activity of long interspersed nuclear element derived microRNA 625 induced by NF-κB Lee, Hee-Eun Park, Sang-Je Huh, Jae-Won Imai, Hiroo Kim, Heui-Soo Sci Rep Article Transposable elements (TEs) are DNA sequences that cut or introduced into the genome, and they represent a massive portion of the human genome. TEs generate a considerable number of microRNAs (miRNAs) are derived from TEs (MDTEs). Numerous miRNAs are related to cancer, and hsa-miRNA-625 is a well-known oncomiR derived from long interspersed nuclear elements (LINEs). The relative expression of hsa-miRNA-625-5p differs in humans, chimpanzees, crab-eating monkeys, and mice, and four primers were designed against the 3′UTR of GATAD2B to analyze the different quantities of canonical binding sites and the location of miRNA binding sites. Luciferase assay was performed to score for the interaction between hsa-miRNA-625 and the 3′UTR of GATAD2B, while blocking NF-κB. In summary, the different numbers of canonical binding sites and the locations of miRNA binding sites affect gene expression, and NF-κB induces the enhancer activity of hsa-miRNA-625-5p by sharing the binding sites. Nature Publishing Group UK 2021-02-04 /pmc/articles/PMC7862687/ /pubmed/33542430 http://dx.doi.org/10.1038/s41598-021-82735-x Text en © The Author(s) 2021 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Lee, Hee-Eun
Park, Sang-Je
Huh, Jae-Won
Imai, Hiroo
Kim, Heui-Soo
The enhancer activity of long interspersed nuclear element derived microRNA 625 induced by NF-κB
title The enhancer activity of long interspersed nuclear element derived microRNA 625 induced by NF-κB
title_full The enhancer activity of long interspersed nuclear element derived microRNA 625 induced by NF-κB
title_fullStr The enhancer activity of long interspersed nuclear element derived microRNA 625 induced by NF-κB
title_full_unstemmed The enhancer activity of long interspersed nuclear element derived microRNA 625 induced by NF-κB
title_short The enhancer activity of long interspersed nuclear element derived microRNA 625 induced by NF-κB
title_sort enhancer activity of long interspersed nuclear element derived microrna 625 induced by nf-κb
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7862687/
https://www.ncbi.nlm.nih.gov/pubmed/33542430
http://dx.doi.org/10.1038/s41598-021-82735-x
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