Cargando…

A decellularized human corneal scaffold for anterior corneal surface reconstruction

Allogenic transplants of the cornea are prone to rejection, especially in repetitive transplantation and in scarred or highly vascularized recipient sites. Patients with these ailments would particularly benefit from the possibility to use non-immunogenic decellularized tissue scaffolds for transpla...

Descripción completa

Detalles Bibliográficos
Autores principales: Polisetti, Naresh, Schmid, Anke, Schlötzer-Schrehardt, Ursula, Maier, Philip, Lang, Stefan J., Steinberg, Thorsten, Schlunck, Günther, Reinhard, Thomas
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7862698/
https://www.ncbi.nlm.nih.gov/pubmed/33542377
http://dx.doi.org/10.1038/s41598-021-82678-3
_version_ 1783647343949643776
author Polisetti, Naresh
Schmid, Anke
Schlötzer-Schrehardt, Ursula
Maier, Philip
Lang, Stefan J.
Steinberg, Thorsten
Schlunck, Günther
Reinhard, Thomas
author_facet Polisetti, Naresh
Schmid, Anke
Schlötzer-Schrehardt, Ursula
Maier, Philip
Lang, Stefan J.
Steinberg, Thorsten
Schlunck, Günther
Reinhard, Thomas
author_sort Polisetti, Naresh
collection PubMed
description Allogenic transplants of the cornea are prone to rejection, especially in repetitive transplantation and in scarred or highly vascularized recipient sites. Patients with these ailments would particularly benefit from the possibility to use non-immunogenic decellularized tissue scaffolds for transplantation, which may be repopulated by host cells in situ or in vitro. So, the aim of this study was to develop a fast and efficient decellularization method for creating a human corneal extracellular matrix scaffold suitable for repopulation with human cells from the corneal limbus. To decellularize human donor corneas, sodium deoxycholate, deoxyribonuclease I, and dextran were assessed to remove cells and nuclei and to control tissue swelling, respectively. We evaluated the decellularization effects on the ultrastructure, optical, mechanical, and biological properties of the human cornea. Scaffold recellularization was studied using primary human limbal epithelial cells, stromal cells, and melanocytes in vitro and a lamellar transplantation approach ex vivo. Our data strongly suggest that this approach allowed the effective removal of cellular and nuclear material in a very short period of time while preserving extracellular matrix proteins, glycosaminoglycans, tissue structure, and optical transmission properties. In vitro recellularization demonstrated good biocompatibility of the decellularized human cornea and ex vivo transplantation revealed complete epithelialization and stromal repopulation from the host tissue. Thus, the generated decellularized human corneal scaffold could be a promising biological material for anterior corneal reconstruction in the treatment of corneal defects.
format Online
Article
Text
id pubmed-7862698
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher Nature Publishing Group UK
record_format MEDLINE/PubMed
spelling pubmed-78626982021-02-08 A decellularized human corneal scaffold for anterior corneal surface reconstruction Polisetti, Naresh Schmid, Anke Schlötzer-Schrehardt, Ursula Maier, Philip Lang, Stefan J. Steinberg, Thorsten Schlunck, Günther Reinhard, Thomas Sci Rep Article Allogenic transplants of the cornea are prone to rejection, especially in repetitive transplantation and in scarred or highly vascularized recipient sites. Patients with these ailments would particularly benefit from the possibility to use non-immunogenic decellularized tissue scaffolds for transplantation, which may be repopulated by host cells in situ or in vitro. So, the aim of this study was to develop a fast and efficient decellularization method for creating a human corneal extracellular matrix scaffold suitable for repopulation with human cells from the corneal limbus. To decellularize human donor corneas, sodium deoxycholate, deoxyribonuclease I, and dextran were assessed to remove cells and nuclei and to control tissue swelling, respectively. We evaluated the decellularization effects on the ultrastructure, optical, mechanical, and biological properties of the human cornea. Scaffold recellularization was studied using primary human limbal epithelial cells, stromal cells, and melanocytes in vitro and a lamellar transplantation approach ex vivo. Our data strongly suggest that this approach allowed the effective removal of cellular and nuclear material in a very short period of time while preserving extracellular matrix proteins, glycosaminoglycans, tissue structure, and optical transmission properties. In vitro recellularization demonstrated good biocompatibility of the decellularized human cornea and ex vivo transplantation revealed complete epithelialization and stromal repopulation from the host tissue. Thus, the generated decellularized human corneal scaffold could be a promising biological material for anterior corneal reconstruction in the treatment of corneal defects. Nature Publishing Group UK 2021-02-04 /pmc/articles/PMC7862698/ /pubmed/33542377 http://dx.doi.org/10.1038/s41598-021-82678-3 Text en © The Author(s) 2021 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Polisetti, Naresh
Schmid, Anke
Schlötzer-Schrehardt, Ursula
Maier, Philip
Lang, Stefan J.
Steinberg, Thorsten
Schlunck, Günther
Reinhard, Thomas
A decellularized human corneal scaffold for anterior corneal surface reconstruction
title A decellularized human corneal scaffold for anterior corneal surface reconstruction
title_full A decellularized human corneal scaffold for anterior corneal surface reconstruction
title_fullStr A decellularized human corneal scaffold for anterior corneal surface reconstruction
title_full_unstemmed A decellularized human corneal scaffold for anterior corneal surface reconstruction
title_short A decellularized human corneal scaffold for anterior corneal surface reconstruction
title_sort decellularized human corneal scaffold for anterior corneal surface reconstruction
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7862698/
https://www.ncbi.nlm.nih.gov/pubmed/33542377
http://dx.doi.org/10.1038/s41598-021-82678-3
work_keys_str_mv AT polisettinaresh adecellularizedhumancornealscaffoldforanteriorcornealsurfacereconstruction
AT schmidanke adecellularizedhumancornealscaffoldforanteriorcornealsurfacereconstruction
AT schlotzerschrehardtursula adecellularizedhumancornealscaffoldforanteriorcornealsurfacereconstruction
AT maierphilip adecellularizedhumancornealscaffoldforanteriorcornealsurfacereconstruction
AT langstefanj adecellularizedhumancornealscaffoldforanteriorcornealsurfacereconstruction
AT steinbergthorsten adecellularizedhumancornealscaffoldforanteriorcornealsurfacereconstruction
AT schlunckgunther adecellularizedhumancornealscaffoldforanteriorcornealsurfacereconstruction
AT reinhardthomas adecellularizedhumancornealscaffoldforanteriorcornealsurfacereconstruction
AT polisettinaresh decellularizedhumancornealscaffoldforanteriorcornealsurfacereconstruction
AT schmidanke decellularizedhumancornealscaffoldforanteriorcornealsurfacereconstruction
AT schlotzerschrehardtursula decellularizedhumancornealscaffoldforanteriorcornealsurfacereconstruction
AT maierphilip decellularizedhumancornealscaffoldforanteriorcornealsurfacereconstruction
AT langstefanj decellularizedhumancornealscaffoldforanteriorcornealsurfacereconstruction
AT steinbergthorsten decellularizedhumancornealscaffoldforanteriorcornealsurfacereconstruction
AT schlunckgunther decellularizedhumancornealscaffoldforanteriorcornealsurfacereconstruction
AT reinhardthomas decellularizedhumancornealscaffoldforanteriorcornealsurfacereconstruction