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A decellularized human corneal scaffold for anterior corneal surface reconstruction
Allogenic transplants of the cornea are prone to rejection, especially in repetitive transplantation and in scarred or highly vascularized recipient sites. Patients with these ailments would particularly benefit from the possibility to use non-immunogenic decellularized tissue scaffolds for transpla...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7862698/ https://www.ncbi.nlm.nih.gov/pubmed/33542377 http://dx.doi.org/10.1038/s41598-021-82678-3 |
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author | Polisetti, Naresh Schmid, Anke Schlötzer-Schrehardt, Ursula Maier, Philip Lang, Stefan J. Steinberg, Thorsten Schlunck, Günther Reinhard, Thomas |
author_facet | Polisetti, Naresh Schmid, Anke Schlötzer-Schrehardt, Ursula Maier, Philip Lang, Stefan J. Steinberg, Thorsten Schlunck, Günther Reinhard, Thomas |
author_sort | Polisetti, Naresh |
collection | PubMed |
description | Allogenic transplants of the cornea are prone to rejection, especially in repetitive transplantation and in scarred or highly vascularized recipient sites. Patients with these ailments would particularly benefit from the possibility to use non-immunogenic decellularized tissue scaffolds for transplantation, which may be repopulated by host cells in situ or in vitro. So, the aim of this study was to develop a fast and efficient decellularization method for creating a human corneal extracellular matrix scaffold suitable for repopulation with human cells from the corneal limbus. To decellularize human donor corneas, sodium deoxycholate, deoxyribonuclease I, and dextran were assessed to remove cells and nuclei and to control tissue swelling, respectively. We evaluated the decellularization effects on the ultrastructure, optical, mechanical, and biological properties of the human cornea. Scaffold recellularization was studied using primary human limbal epithelial cells, stromal cells, and melanocytes in vitro and a lamellar transplantation approach ex vivo. Our data strongly suggest that this approach allowed the effective removal of cellular and nuclear material in a very short period of time while preserving extracellular matrix proteins, glycosaminoglycans, tissue structure, and optical transmission properties. In vitro recellularization demonstrated good biocompatibility of the decellularized human cornea and ex vivo transplantation revealed complete epithelialization and stromal repopulation from the host tissue. Thus, the generated decellularized human corneal scaffold could be a promising biological material for anterior corneal reconstruction in the treatment of corneal defects. |
format | Online Article Text |
id | pubmed-7862698 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-78626982021-02-08 A decellularized human corneal scaffold for anterior corneal surface reconstruction Polisetti, Naresh Schmid, Anke Schlötzer-Schrehardt, Ursula Maier, Philip Lang, Stefan J. Steinberg, Thorsten Schlunck, Günther Reinhard, Thomas Sci Rep Article Allogenic transplants of the cornea are prone to rejection, especially in repetitive transplantation and in scarred or highly vascularized recipient sites. Patients with these ailments would particularly benefit from the possibility to use non-immunogenic decellularized tissue scaffolds for transplantation, which may be repopulated by host cells in situ or in vitro. So, the aim of this study was to develop a fast and efficient decellularization method for creating a human corneal extracellular matrix scaffold suitable for repopulation with human cells from the corneal limbus. To decellularize human donor corneas, sodium deoxycholate, deoxyribonuclease I, and dextran were assessed to remove cells and nuclei and to control tissue swelling, respectively. We evaluated the decellularization effects on the ultrastructure, optical, mechanical, and biological properties of the human cornea. Scaffold recellularization was studied using primary human limbal epithelial cells, stromal cells, and melanocytes in vitro and a lamellar transplantation approach ex vivo. Our data strongly suggest that this approach allowed the effective removal of cellular and nuclear material in a very short period of time while preserving extracellular matrix proteins, glycosaminoglycans, tissue structure, and optical transmission properties. In vitro recellularization demonstrated good biocompatibility of the decellularized human cornea and ex vivo transplantation revealed complete epithelialization and stromal repopulation from the host tissue. Thus, the generated decellularized human corneal scaffold could be a promising biological material for anterior corneal reconstruction in the treatment of corneal defects. Nature Publishing Group UK 2021-02-04 /pmc/articles/PMC7862698/ /pubmed/33542377 http://dx.doi.org/10.1038/s41598-021-82678-3 Text en © The Author(s) 2021 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Polisetti, Naresh Schmid, Anke Schlötzer-Schrehardt, Ursula Maier, Philip Lang, Stefan J. Steinberg, Thorsten Schlunck, Günther Reinhard, Thomas A decellularized human corneal scaffold for anterior corneal surface reconstruction |
title | A decellularized human corneal scaffold for anterior corneal surface reconstruction |
title_full | A decellularized human corneal scaffold for anterior corneal surface reconstruction |
title_fullStr | A decellularized human corneal scaffold for anterior corneal surface reconstruction |
title_full_unstemmed | A decellularized human corneal scaffold for anterior corneal surface reconstruction |
title_short | A decellularized human corneal scaffold for anterior corneal surface reconstruction |
title_sort | decellularized human corneal scaffold for anterior corneal surface reconstruction |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7862698/ https://www.ncbi.nlm.nih.gov/pubmed/33542377 http://dx.doi.org/10.1038/s41598-021-82678-3 |
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