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Anti-Foc RT4 Activity of a Newly Isolated Streptomyces sp. 5–10 From a Medicinal Plant (Curculigo capitulata)

Fusarium wilt of banana caused by Fusarium oxysporum f. sp. cubense (Foc) is a disastrous soil-borne fungal disease. Foc tropical race 4 (Foc TR4) can infect almost all banana cultivars. Until now, there is a shortage of safety and effective control methods and commercial banana cultivars with a res...

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Autores principales: Yun, Tianyan, Zhang, Miaoyi, Zhou, Dengbo, Jing, Tao, Zang, Xiaoping, Qi, Dengfeng, Chen, Yufeng, Li, Kai, Zhao, Yankun, Tang, Wen, Huang, Jiaquan, Wang, Wei, Xie, Jianghui
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7862724/
https://www.ncbi.nlm.nih.gov/pubmed/33552022
http://dx.doi.org/10.3389/fmicb.2020.610698
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author Yun, Tianyan
Zhang, Miaoyi
Zhou, Dengbo
Jing, Tao
Zang, Xiaoping
Qi, Dengfeng
Chen, Yufeng
Li, Kai
Zhao, Yankun
Tang, Wen
Huang, Jiaquan
Wang, Wei
Xie, Jianghui
author_facet Yun, Tianyan
Zhang, Miaoyi
Zhou, Dengbo
Jing, Tao
Zang, Xiaoping
Qi, Dengfeng
Chen, Yufeng
Li, Kai
Zhao, Yankun
Tang, Wen
Huang, Jiaquan
Wang, Wei
Xie, Jianghui
author_sort Yun, Tianyan
collection PubMed
description Fusarium wilt of banana caused by Fusarium oxysporum f. sp. cubense (Foc) is a disastrous soil-borne fungal disease. Foc tropical race 4 (Foc TR4) can infect almost all banana cultivars. Until now, there is a shortage of safety and effective control methods and commercial banana cultivars with a resistance against Foc TR4. Biocontrol using environmentally friendly microbes is a promising strategy for the management of Foc TR4. Here, a strain 5–10, newly isolated from a medicinal plant (Curculigo capitulata), exhibited a high antifungal activity against Foc TR4. Combing the morphological characteristics and molecular identification, strain 5–10 was classified as a Streptomyces genus. The sequenced genome revealed that more than 39 gene clusters were involved in the biosynthesis of secondary metabolites. Some multidrug resistance gene clusters were also identified such as mdtD, vatB, and vgaE. To improve the anti-Foc TR4 activity of the strain 5–10 extracts, an optimization method of fermentation broth was established. Antifungal activity increased by 72.13% under the fermentation system containing 2.86 g/L of NaCl and 11.57% of inoculation amount. After being treated with the strain 5–10 extracts, the Foc TR4 hyphae shrinked, deformed, and ruptured. The membrane integrity and cell ultrastructure incurred irreversible damage. Streptomyces sp. 5–10 extracts play a fungicidal role in Foc TR4. Hence, Streptomyces sp. 5–10 will be a potential biocontrol agent to manage fungal diseases by exploring the microbial fertilizer.
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spelling pubmed-78627242021-02-06 Anti-Foc RT4 Activity of a Newly Isolated Streptomyces sp. 5–10 From a Medicinal Plant (Curculigo capitulata) Yun, Tianyan Zhang, Miaoyi Zhou, Dengbo Jing, Tao Zang, Xiaoping Qi, Dengfeng Chen, Yufeng Li, Kai Zhao, Yankun Tang, Wen Huang, Jiaquan Wang, Wei Xie, Jianghui Front Microbiol Microbiology Fusarium wilt of banana caused by Fusarium oxysporum f. sp. cubense (Foc) is a disastrous soil-borne fungal disease. Foc tropical race 4 (Foc TR4) can infect almost all banana cultivars. Until now, there is a shortage of safety and effective control methods and commercial banana cultivars with a resistance against Foc TR4. Biocontrol using environmentally friendly microbes is a promising strategy for the management of Foc TR4. Here, a strain 5–10, newly isolated from a medicinal plant (Curculigo capitulata), exhibited a high antifungal activity against Foc TR4. Combing the morphological characteristics and molecular identification, strain 5–10 was classified as a Streptomyces genus. The sequenced genome revealed that more than 39 gene clusters were involved in the biosynthesis of secondary metabolites. Some multidrug resistance gene clusters were also identified such as mdtD, vatB, and vgaE. To improve the anti-Foc TR4 activity of the strain 5–10 extracts, an optimization method of fermentation broth was established. Antifungal activity increased by 72.13% under the fermentation system containing 2.86 g/L of NaCl and 11.57% of inoculation amount. After being treated with the strain 5–10 extracts, the Foc TR4 hyphae shrinked, deformed, and ruptured. The membrane integrity and cell ultrastructure incurred irreversible damage. Streptomyces sp. 5–10 extracts play a fungicidal role in Foc TR4. Hence, Streptomyces sp. 5–10 will be a potential biocontrol agent to manage fungal diseases by exploring the microbial fertilizer. Frontiers Media S.A. 2021-01-22 /pmc/articles/PMC7862724/ /pubmed/33552022 http://dx.doi.org/10.3389/fmicb.2020.610698 Text en Copyright © 2021 Yun, Zhang, Zhou, Jing, Zang, Qi, Chen, Li, Zhao, Tang, Huang, Wang and Xie. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Yun, Tianyan
Zhang, Miaoyi
Zhou, Dengbo
Jing, Tao
Zang, Xiaoping
Qi, Dengfeng
Chen, Yufeng
Li, Kai
Zhao, Yankun
Tang, Wen
Huang, Jiaquan
Wang, Wei
Xie, Jianghui
Anti-Foc RT4 Activity of a Newly Isolated Streptomyces sp. 5–10 From a Medicinal Plant (Curculigo capitulata)
title Anti-Foc RT4 Activity of a Newly Isolated Streptomyces sp. 5–10 From a Medicinal Plant (Curculigo capitulata)
title_full Anti-Foc RT4 Activity of a Newly Isolated Streptomyces sp. 5–10 From a Medicinal Plant (Curculigo capitulata)
title_fullStr Anti-Foc RT4 Activity of a Newly Isolated Streptomyces sp. 5–10 From a Medicinal Plant (Curculigo capitulata)
title_full_unstemmed Anti-Foc RT4 Activity of a Newly Isolated Streptomyces sp. 5–10 From a Medicinal Plant (Curculigo capitulata)
title_short Anti-Foc RT4 Activity of a Newly Isolated Streptomyces sp. 5–10 From a Medicinal Plant (Curculigo capitulata)
title_sort anti-foc rt4 activity of a newly isolated streptomyces sp. 5–10 from a medicinal plant (curculigo capitulata)
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7862724/
https://www.ncbi.nlm.nih.gov/pubmed/33552022
http://dx.doi.org/10.3389/fmicb.2020.610698
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