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miR-142-5p promotes cervical cancer progression by targeting LMX1A through Wnt/β-catenin pathway
BACKGROUND: Previous work has shown that miR-142-5p in cervical cancer tissues increased significantly compared with adjacent normal tissues. However, the function and the mechanism of miR-142-5p in cervical cancer have not been reported. METHODS: Quantitative reverse transcription-polymerase chain...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
De Gruyter
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7862994/ https://www.ncbi.nlm.nih.gov/pubmed/33585699 http://dx.doi.org/10.1515/med-2021-0218 |
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author | Ke, Lijuan Chen, Yanping Li, Yiying Chen, Zheng He, Yihui Liu, Jiahua Zhuang, Yingfeng |
author_facet | Ke, Lijuan Chen, Yanping Li, Yiying Chen, Zheng He, Yihui Liu, Jiahua Zhuang, Yingfeng |
author_sort | Ke, Lijuan |
collection | PubMed |
description | BACKGROUND: Previous work has shown that miR-142-5p in cervical cancer tissues increased significantly compared with adjacent normal tissues. However, the function and the mechanism of miR-142-5p in cervical cancer have not been reported. METHODS: Quantitative reverse transcription-polymerase chain reaction (qRT-PCR) was used to determine the gene expression levels. MTT, flow cytometry, and transwell assays were performed to explore the functions of miR-142-5p in HeLa cells. The potential target gene of miR-142-5p was investigated via luciferase reporter assays. The protein expression levels were analyzed by Western blotting. RESULTS: We found that miR-142-5p expression was elevated but LIM homeobox transcription factor 1 alpha (LMX1A) was decreased in cervical cancer tissues and cells. Overexpression of miR-142-5p or knockdown of LMX1A inhibited cell apoptosis, promoted cell proliferation, migration, invasion abilities, and activated the Wnt/β-catenin pathway. However, knockdown of miR-142-5p or overexpression of LMX1A showed opposite results. LMX1A was identified as a direct target of miR-142-5p by luciferase reporter assays. Finally, rescue experiments demonstrated that LMX1A overexpression attenuated the carcinogenic effect of miR-142-5p mimic on HeLa cells. CONCLUSIONS: These findings suggested that miR-142-5p might be a cervical cancer oncogene and could serve as a potential therapeutic target for the treatment of cervical cancer. |
format | Online Article Text |
id | pubmed-7862994 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | De Gruyter |
record_format | MEDLINE/PubMed |
spelling | pubmed-78629942021-02-12 miR-142-5p promotes cervical cancer progression by targeting LMX1A through Wnt/β-catenin pathway Ke, Lijuan Chen, Yanping Li, Yiying Chen, Zheng He, Yihui Liu, Jiahua Zhuang, Yingfeng Open Med (Wars) Research Article BACKGROUND: Previous work has shown that miR-142-5p in cervical cancer tissues increased significantly compared with adjacent normal tissues. However, the function and the mechanism of miR-142-5p in cervical cancer have not been reported. METHODS: Quantitative reverse transcription-polymerase chain reaction (qRT-PCR) was used to determine the gene expression levels. MTT, flow cytometry, and transwell assays were performed to explore the functions of miR-142-5p in HeLa cells. The potential target gene of miR-142-5p was investigated via luciferase reporter assays. The protein expression levels were analyzed by Western blotting. RESULTS: We found that miR-142-5p expression was elevated but LIM homeobox transcription factor 1 alpha (LMX1A) was decreased in cervical cancer tissues and cells. Overexpression of miR-142-5p or knockdown of LMX1A inhibited cell apoptosis, promoted cell proliferation, migration, invasion abilities, and activated the Wnt/β-catenin pathway. However, knockdown of miR-142-5p or overexpression of LMX1A showed opposite results. LMX1A was identified as a direct target of miR-142-5p by luciferase reporter assays. Finally, rescue experiments demonstrated that LMX1A overexpression attenuated the carcinogenic effect of miR-142-5p mimic on HeLa cells. CONCLUSIONS: These findings suggested that miR-142-5p might be a cervical cancer oncogene and could serve as a potential therapeutic target for the treatment of cervical cancer. De Gruyter 2021-01-28 /pmc/articles/PMC7862994/ /pubmed/33585699 http://dx.doi.org/10.1515/med-2021-0218 Text en © 2021 Lijuan Ke et al., published by De Gruyter http://creativecommons.org/licenses/by/4.0 This work is licensed under the Creative Commons Attribution 4.0 International License. |
spellingShingle | Research Article Ke, Lijuan Chen, Yanping Li, Yiying Chen, Zheng He, Yihui Liu, Jiahua Zhuang, Yingfeng miR-142-5p promotes cervical cancer progression by targeting LMX1A through Wnt/β-catenin pathway |
title | miR-142-5p promotes cervical cancer progression by targeting LMX1A through Wnt/β-catenin pathway |
title_full | miR-142-5p promotes cervical cancer progression by targeting LMX1A through Wnt/β-catenin pathway |
title_fullStr | miR-142-5p promotes cervical cancer progression by targeting LMX1A through Wnt/β-catenin pathway |
title_full_unstemmed | miR-142-5p promotes cervical cancer progression by targeting LMX1A through Wnt/β-catenin pathway |
title_short | miR-142-5p promotes cervical cancer progression by targeting LMX1A through Wnt/β-catenin pathway |
title_sort | mir-142-5p promotes cervical cancer progression by targeting lmx1a through wnt/β-catenin pathway |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7862994/ https://www.ncbi.nlm.nih.gov/pubmed/33585699 http://dx.doi.org/10.1515/med-2021-0218 |
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