Cargando…

Building better polymerases: Engineering the replication of expanded genetic alphabets

DNA polymerases are today used throughout scientific research, biotechnology, and medicine, in part for their ability to interact with unnatural forms of DNA created by synthetic biologists. Here especially, natural DNA polymerases often do not have the “performance specifications” needed for transf...

Descripción completa

Detalles Bibliográficos
Autores principales:	Ouaray, Zahra, Benner, Steven A., Georgiadis, Millie M., Richards, Nigel G. J.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	American Society for Biochemistry and Molecular Biology 2020
Materias:	JBC Reviews
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7863901/ https://www.ncbi.nlm.nih.gov/pubmed/33004440 http://dx.doi.org/10.1074/jbc.REV120.013745

Ejemplares similares

Building better enzymes: Molecular basis of improved non‐natural nucleobase incorporation by an evolved DNA polymerase
por: Ouaray, Zahra, et al.
Publicado: (2019)

Recognition of an expanded genetic alphabet by type-II restriction endonucleases and their application to analyze polymerase fidelity
por: Chen, Fei, et al.
Publicado: (2011)

In vitro evolution of ribonucleases from expanded genetic alphabets
por: Jerome, Craig A., et al.
Publicado: (2022)

Multiplexed Genetic Analysis Using an Expanded Genetic Alphabet
por: Johnson, Scott C, et al.
Publicado: (2004)

Detecting respiratory viral RNA using expanded genetic alphabets and self-avoiding DNA
por: Glushakova, Lyudmyla G., et al.
Publicado: (2015)

Nucleotide addition and cleavage by RNA polymerase II: Coordination of two catalytic reactions using a single active site
por: Unarta, Ilona Christy, et al.
Publicado: (2022)

Replication of the coronavirus genome: A paradox among positive-strand RNA viruses
por: Grellet, Emeline, et al.
Publicado: (2022)

Engineering enzyme activity using an expanded amino acid alphabet
por: Birch-Price, Zachary, et al.
Publicado: (2022)

A Semi-Synthetic Organism with an Expanded Genetic Alphabet
por: Malyshev, Denis A., et al.
Publicado: (2014)

Engineering functional thermostable proteins using ancestral sequence reconstruction
por: Thomson, Raine E.S., et al.
Publicado: (2022)

Genetic glycoengineering in mammalian cells
por: Narimatsu, Yoshiki, et al.
Publicado: (2021)

Snapshots of an evolved DNA polymerase pre- and post-incorporation of an unnatural nucleotide
por: Singh, Isha, et al.
Publicado: (2018)

Phenotypic analysis with trans-recombination–based genetic mosaic models
por: Zhang, Yu, et al.
Publicado: (2023)

Limb blastema formation: How much do we know at a genetic and epigenetic level?
por: Min, Sangwon, et al.
Publicado: (2022)

Crystal structures of ‘ALternative Isoinformational ENgineered’ DNA in B-form
por: Shukla, Madhura S., et al.
Publicado: (2023)

A genetic approach for building different alphabets for peptide and protein classification
por: Nanni, Loris, et al.
Publicado: (2008)

Expanding the BLUP alphabet for genomic prediction adaptable to the genetic architectures of complex traits
por: Wang, Jiabo, et al.
Publicado: (2018)

Redundant electrostatic interactions between GATOR1 and the Rag GTPase heterodimer drive efficient amino acid sensing in human cells
por: Doxsey, Dylan D., et al.
Publicado: (2023)

Autonomous assembly of synthetic oligonucleotides built from an expanded DNA alphabet. Total synthesis of a gene encoding kanamycin resistance
por: Merritt, Kristen K, et al.
Publicado: (2014)

DNA Structure Design Is Improved Using an Artificially Expanded Alphabet of Base Pairs Including Loop and Mismatch Thermodynamic Parameters
por: Pham, Tuan M., et al.
Publicado: (2023)

DNA Structure Design Is Improved Using an Artificially Expanded Alphabet of Base Pairs Including Loop and Mismatch Thermodynamic Parameters
por: Pham, Tuan M., et al.
Publicado: (2023)

Fatty acid recognition in the Frizzled receptor family
por: Nile, Aaron H., et al.
Publicado: (2019)

Polyadenylation and nuclear export of mRNAs
por: Stewart, Murray
Publicado: (2019)

Role of the lipid bilayer in outer membrane protein folding in Gram-negative bacteria
por: Horne, Jim E., et al.
Publicado: (2020)

A molecular roadmap to the plant immune system
por: Bentham, Adam R., et al.
Publicado: (2020)

Regulatory effects of post-translational modifications on zDHHC S-acyltransferases
por: Zmuda, Filip, et al.
Publicado: (2020)

The molecular virology of coronaviruses
por: Hartenian, Ella, et al.
Publicado: (2020)

Biosynthesis of the modified tetrapyrroles—the pigments of life
por: Bryant, Donald A., et al.
Publicado: (2020)

Strategies for site-specific recombination with high efficiency and precise spatiotemporal resolution
por: Tian, Xueying, et al.
Publicado: (2021)

Is γ-secretase a beneficial inactivating enzyme of the toxic APP C-terminal fragment C99?
por: Checler, Frédéric, et al.
Publicado: (2021)

The PDB and the ribosome
por: Moore, Peter B.
Publicado: (2021)

The scrambled story between hyaluronan and glioblastoma
por: Pibuel, Matías Arturo, et al.
Publicado: (2021)

Evolution of standardization and dissemination of cryo-EM structures and data jointly by the community, PDB, and EMDB
por: Chiu, Wah, et al.
Publicado: (2021)

Recent advances in de novo protein design: Principles, methods, and applications
por: Pan, Xingjie, et al.
Publicado: (2021)

Impact of structural biologists and the Protein Data Bank on small-molecule drug discovery and development
por: Burley, Stephen K.
Publicado: (2021)

RHO to the DOCK for GDP disembarking: Structural insights into the DOCK GTPase nucleotide exchange factors
por: Thompson, Andrew P., et al.
Publicado: (2021)

Beyond the double helix: DNA structural diversity and the PDB
por: Neidle, Stephen
Publicado: (2021)

A perspective on the PDB’s impact on the field of glycobiology
por: Prestegard, James H.
Publicado: (2021)

Bacterial iron detoxification at the molecular level
por: Bradley, Justin M., et al.
Publicado: (2020)

Visualizing, quantifying, and manipulating mitochondrial DNA in vivo
por: Prole, David L., et al.
Publicado: (2020)

Cannot write session to /tmp/vufind_sessions/sess_381d9nh51slha456dinbn1ajah