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The Clinical Significance and Potential Molecular Mechanism of PTTG1 in Esophageal Squamous Cell Carcinoma

Esophageal squamous cell carcinoma (ESCC) is the major histological type of esophageal cancers worldwide. Transcription factor PTTG1 was seen highly expressed in a variety of tumors and was related to the degree of tumor differentiation, invasion, and metastasis. However, the clinical significance o...

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Autores principales: Chen, Shang-Wei, Zhou, Hua-Fu, Zhang, Han-Jie, He, Rong-Quan, Huang, Zhi-Guang, Dang, Yi-Wu, Yang, Xia, Liu, Jun, Fu, Zong-Wang, Mo, Jun-Xian, Tang, Zhong-Qing, Li, Chang-Bo, Li, Rong, Yang, Li-Hua, Ma, Jie, Yang, Lin-Jie, Chen, Gang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7863988/
https://www.ncbi.nlm.nih.gov/pubmed/33552118
http://dx.doi.org/10.3389/fgene.2020.583085
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author Chen, Shang-Wei
Zhou, Hua-Fu
Zhang, Han-Jie
He, Rong-Quan
Huang, Zhi-Guang
Dang, Yi-Wu
Yang, Xia
Liu, Jun
Fu, Zong-Wang
Mo, Jun-Xian
Tang, Zhong-Qing
Li, Chang-Bo
Li, Rong
Yang, Li-Hua
Ma, Jie
Yang, Lin-Jie
Chen, Gang
author_facet Chen, Shang-Wei
Zhou, Hua-Fu
Zhang, Han-Jie
He, Rong-Quan
Huang, Zhi-Guang
Dang, Yi-Wu
Yang, Xia
Liu, Jun
Fu, Zong-Wang
Mo, Jun-Xian
Tang, Zhong-Qing
Li, Chang-Bo
Li, Rong
Yang, Li-Hua
Ma, Jie
Yang, Lin-Jie
Chen, Gang
author_sort Chen, Shang-Wei
collection PubMed
description Esophageal squamous cell carcinoma (ESCC) is the major histological type of esophageal cancers worldwide. Transcription factor PTTG1 was seen highly expressed in a variety of tumors and was related to the degree of tumor differentiation, invasion, and metastasis. However, the clinical significance of PTTG1 had yet to be verified, and the mechanism of abnormal PTTG1 expression in ESCC was not clear. In this study, the comprehensive analysis and evaluation of PTTG1 expression in ESCC were completed by synthesizing in-house immunohistochemistry (IHC), clinical sample tissue RNA-seq (in-house RNA-seq), public high-throughput data, and literature data. We also explored the possible signaling pathways and target genes of PTTG1 in ESCC by combining the target genes of PTTG1 (displayed by ChIP-seq), differentially expressed genes (DEGs) of ESCC, and PTTG1-related genes, revealing the potential molecular mechanism of PTTG1 in ESCC. In the present study, PTTG1 protein and mRNA expression levels in ESCC tissues were all significantly higher than in non-cancerous tissues. The pool standard mean difference (SMD) of the overall PTTG1 expression was 1.17 (95% CI: 0.72–1.62, P < 0.01), and the area under curve (AUC) of the summary receiver operating characteristic (SROC) was 0.86 (95% CI: 0.83–0.89). By combining the target genes displayed by ChIP-seq of PTTG1, DEGs of ESCC, and PTTG1-related genes, it was observed that PTTG1 may interact with these genes through chemokines and cytokine signaling pathways. By constructing a protein–protein interaction (PPI) network and combining ChIP-seq data, we obtained four PTTG1 potential target genes, SPTAN1, SLC25A17, IKBKB, and ERH. The gene expression of PTTG1 had a strong positive correlation with SLC25A17 and ERH, which suggested that PTTG1 might positively regulate the expression of these two genes. In summary, the high expression of PTTG1 may play an important role in the formation of ESCC. These roles may be completed by PTTG1 regulating the downstream target genes SLC25A17 and ERH.
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spelling pubmed-78639882021-02-06 The Clinical Significance and Potential Molecular Mechanism of PTTG1 in Esophageal Squamous Cell Carcinoma Chen, Shang-Wei Zhou, Hua-Fu Zhang, Han-Jie He, Rong-Quan Huang, Zhi-Guang Dang, Yi-Wu Yang, Xia Liu, Jun Fu, Zong-Wang Mo, Jun-Xian Tang, Zhong-Qing Li, Chang-Bo Li, Rong Yang, Li-Hua Ma, Jie Yang, Lin-Jie Chen, Gang Front Genet Genetics Esophageal squamous cell carcinoma (ESCC) is the major histological type of esophageal cancers worldwide. Transcription factor PTTG1 was seen highly expressed in a variety of tumors and was related to the degree of tumor differentiation, invasion, and metastasis. However, the clinical significance of PTTG1 had yet to be verified, and the mechanism of abnormal PTTG1 expression in ESCC was not clear. In this study, the comprehensive analysis and evaluation of PTTG1 expression in ESCC were completed by synthesizing in-house immunohistochemistry (IHC), clinical sample tissue RNA-seq (in-house RNA-seq), public high-throughput data, and literature data. We also explored the possible signaling pathways and target genes of PTTG1 in ESCC by combining the target genes of PTTG1 (displayed by ChIP-seq), differentially expressed genes (DEGs) of ESCC, and PTTG1-related genes, revealing the potential molecular mechanism of PTTG1 in ESCC. In the present study, PTTG1 protein and mRNA expression levels in ESCC tissues were all significantly higher than in non-cancerous tissues. The pool standard mean difference (SMD) of the overall PTTG1 expression was 1.17 (95% CI: 0.72–1.62, P < 0.01), and the area under curve (AUC) of the summary receiver operating characteristic (SROC) was 0.86 (95% CI: 0.83–0.89). By combining the target genes displayed by ChIP-seq of PTTG1, DEGs of ESCC, and PTTG1-related genes, it was observed that PTTG1 may interact with these genes through chemokines and cytokine signaling pathways. By constructing a protein–protein interaction (PPI) network and combining ChIP-seq data, we obtained four PTTG1 potential target genes, SPTAN1, SLC25A17, IKBKB, and ERH. The gene expression of PTTG1 had a strong positive correlation with SLC25A17 and ERH, which suggested that PTTG1 might positively regulate the expression of these two genes. In summary, the high expression of PTTG1 may play an important role in the formation of ESCC. These roles may be completed by PTTG1 regulating the downstream target genes SLC25A17 and ERH. Frontiers Media S.A. 2021-01-22 /pmc/articles/PMC7863988/ /pubmed/33552118 http://dx.doi.org/10.3389/fgene.2020.583085 Text en Copyright © 2021 Chen, Zhou, Zhang, He, Huang, Dang, Yang, Liu, Fu, Mo, Tang, Li, Li, Yang, Ma, Yang and Chen. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Genetics
Chen, Shang-Wei
Zhou, Hua-Fu
Zhang, Han-Jie
He, Rong-Quan
Huang, Zhi-Guang
Dang, Yi-Wu
Yang, Xia
Liu, Jun
Fu, Zong-Wang
Mo, Jun-Xian
Tang, Zhong-Qing
Li, Chang-Bo
Li, Rong
Yang, Li-Hua
Ma, Jie
Yang, Lin-Jie
Chen, Gang
The Clinical Significance and Potential Molecular Mechanism of PTTG1 in Esophageal Squamous Cell Carcinoma
title The Clinical Significance and Potential Molecular Mechanism of PTTG1 in Esophageal Squamous Cell Carcinoma
title_full The Clinical Significance and Potential Molecular Mechanism of PTTG1 in Esophageal Squamous Cell Carcinoma
title_fullStr The Clinical Significance and Potential Molecular Mechanism of PTTG1 in Esophageal Squamous Cell Carcinoma
title_full_unstemmed The Clinical Significance and Potential Molecular Mechanism of PTTG1 in Esophageal Squamous Cell Carcinoma
title_short The Clinical Significance and Potential Molecular Mechanism of PTTG1 in Esophageal Squamous Cell Carcinoma
title_sort clinical significance and potential molecular mechanism of pttg1 in esophageal squamous cell carcinoma
topic Genetics
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7863988/
https://www.ncbi.nlm.nih.gov/pubmed/33552118
http://dx.doi.org/10.3389/fgene.2020.583085
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