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Early detection of tumor cells in bone marrow and peripheral blood in a fast-progressing gastric cancer model

Helicobacter pylori (H. pylori) infection is a major risk factor for the development of gastric cancer. The authors previously demonstrated that in mice deficient in myeloid differentiation primary response 88 (Myd88(−/−)), infection with Helicobacter felis (H. felis) a close relative of H. pylori,...

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Autores principales: Bali, Prerna, Lozano-Pope, Ivonne, Pachow, Collin, Obonyo, Marygorret
Formato: Online Artículo Texto
Lenguaje:English
Publicado: D.A. Spandidos 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7864146/
https://www.ncbi.nlm.nih.gov/pubmed/33469673
http://dx.doi.org/10.3892/ijo.2021.5171
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author Bali, Prerna
Lozano-Pope, Ivonne
Pachow, Collin
Obonyo, Marygorret
author_facet Bali, Prerna
Lozano-Pope, Ivonne
Pachow, Collin
Obonyo, Marygorret
author_sort Bali, Prerna
collection PubMed
description Helicobacter pylori (H. pylori) infection is a major risk factor for the development of gastric cancer. The authors previously demonstrated that in mice deficient in myeloid differentiation primary response 88 (Myd88(−/−)), infection with Helicobacter felis (H. felis) a close relative of H. pylori, subsequently rapidly progressed to neoplasia. The present study examined circulating tumor cells (CTCs) by measuring the expression of cytokeratins, epithelial-to-mesenchymal transition (EMT)-related markers and cancer stem cell (CSC) markers in bone marrow and peripheral blood from Myd88(−/−) and wild-type (WT) mice. Cytokeratins CK8/18 were detected as early as 4 months post-infection in Myd88(−/−) mice. By contrast, cytokeratins were not detected in WT mice even after 7 months post-infection. The expression of Mucin-1 (MUC1) was observed in both bone marrow and peripheral blood at different time points, suggesting its role in gastric cancer metastasis. Snail, Twist and ZEB were expressed at different levels in bone marrow and peripheral blood. The expression of these EMT-related markers suggests the manifestation of cancer metastasis in the early stages of disease development. LGR5, CD44 and CD133 were the most prominent CSC markers detected. The detection of CSC and EMT markers along with cytokeratins does reinforce their use as biomarkers for gastric cancer metastasis. This early detection of markers suggests that CTCs leave primary site even before cancer is well established. Thus, cytokeratins, EMT, and CSCs could be used as biomarkers to detect aggressive forms of gastric cancers. This information may prove to be of significance in stratifying patients for treatment prior to the onset of severe disease-related characteristics.
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spelling pubmed-78641462021-02-27 Early detection of tumor cells in bone marrow and peripheral blood in a fast-progressing gastric cancer model Bali, Prerna Lozano-Pope, Ivonne Pachow, Collin Obonyo, Marygorret Int J Oncol Articles Helicobacter pylori (H. pylori) infection is a major risk factor for the development of gastric cancer. The authors previously demonstrated that in mice deficient in myeloid differentiation primary response 88 (Myd88(−/−)), infection with Helicobacter felis (H. felis) a close relative of H. pylori, subsequently rapidly progressed to neoplasia. The present study examined circulating tumor cells (CTCs) by measuring the expression of cytokeratins, epithelial-to-mesenchymal transition (EMT)-related markers and cancer stem cell (CSC) markers in bone marrow and peripheral blood from Myd88(−/−) and wild-type (WT) mice. Cytokeratins CK8/18 were detected as early as 4 months post-infection in Myd88(−/−) mice. By contrast, cytokeratins were not detected in WT mice even after 7 months post-infection. The expression of Mucin-1 (MUC1) was observed in both bone marrow and peripheral blood at different time points, suggesting its role in gastric cancer metastasis. Snail, Twist and ZEB were expressed at different levels in bone marrow and peripheral blood. The expression of these EMT-related markers suggests the manifestation of cancer metastasis in the early stages of disease development. LGR5, CD44 and CD133 were the most prominent CSC markers detected. The detection of CSC and EMT markers along with cytokeratins does reinforce their use as biomarkers for gastric cancer metastasis. This early detection of markers suggests that CTCs leave primary site even before cancer is well established. Thus, cytokeratins, EMT, and CSCs could be used as biomarkers to detect aggressive forms of gastric cancers. This information may prove to be of significance in stratifying patients for treatment prior to the onset of severe disease-related characteristics. D.A. Spandidos 2021-01-12 /pmc/articles/PMC7864146/ /pubmed/33469673 http://dx.doi.org/10.3892/ijo.2021.5171 Text en Copyright: © Bali et al. This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License (https://creativecommons.org/licenses/by-nc-nd/4.0/) , which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.
spellingShingle Articles
Bali, Prerna
Lozano-Pope, Ivonne
Pachow, Collin
Obonyo, Marygorret
Early detection of tumor cells in bone marrow and peripheral blood in a fast-progressing gastric cancer model
title Early detection of tumor cells in bone marrow and peripheral blood in a fast-progressing gastric cancer model
title_full Early detection of tumor cells in bone marrow and peripheral blood in a fast-progressing gastric cancer model
title_fullStr Early detection of tumor cells in bone marrow and peripheral blood in a fast-progressing gastric cancer model
title_full_unstemmed Early detection of tumor cells in bone marrow and peripheral blood in a fast-progressing gastric cancer model
title_short Early detection of tumor cells in bone marrow and peripheral blood in a fast-progressing gastric cancer model
title_sort early detection of tumor cells in bone marrow and peripheral blood in a fast-progressing gastric cancer model
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7864146/
https://www.ncbi.nlm.nih.gov/pubmed/33469673
http://dx.doi.org/10.3892/ijo.2021.5171
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