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Extracellular and intracellular intermittent magnetic-fluid hyperthermia treatment of SK-Hep1 hepatocellular carcinoma cells based on magnetic nanoparticles coated with polystyrene sulfonic acid

The use of magnetic nanoparticles (MNPs) magnetized on applying an alternating magnetic field (AMF) to stimulate the thermal characteristics and to induce tumor apoptosis is a currently active area of research in cancer treatment. In previous work, we developed biocompatible and superparamagnetic po...

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Autores principales: Chen, Bo-Wei, Chiu, Guo-Wei, He, Yun-Chi, Huang, Chih-Yu, Huang, Hao-Ting, Sung, Shian-Ying, Hsieh, Chia-Ling, Chang, Wei-Chieh, Hsu, Ming-Shinn, Wei, Zung-Hang, Yao, Da-Jeng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7864458/
https://www.ncbi.nlm.nih.gov/pubmed/33544751
http://dx.doi.org/10.1371/journal.pone.0245286
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author Chen, Bo-Wei
Chiu, Guo-Wei
He, Yun-Chi
Huang, Chih-Yu
Huang, Hao-Ting
Sung, Shian-Ying
Hsieh, Chia-Ling
Chang, Wei-Chieh
Hsu, Ming-Shinn
Wei, Zung-Hang
Yao, Da-Jeng
author_facet Chen, Bo-Wei
Chiu, Guo-Wei
He, Yun-Chi
Huang, Chih-Yu
Huang, Hao-Ting
Sung, Shian-Ying
Hsieh, Chia-Ling
Chang, Wei-Chieh
Hsu, Ming-Shinn
Wei, Zung-Hang
Yao, Da-Jeng
author_sort Chen, Bo-Wei
collection PubMed
description The use of magnetic nanoparticles (MNPs) magnetized on applying an alternating magnetic field (AMF) to stimulate the thermal characteristics and to induce tumor apoptosis is a currently active area of research in cancer treatment. In previous work, we developed biocompatible and superparamagnetic polystyrene-sulfonic-acid-coated magnetic nanoparticles (PSS-MNPs) as applications for magnetically labeled cell trapping, but without assessment of treatment effects on tumor diseases. In the present work, we examined PSS-MNP-induced magnetic fluid hyperthermia (MFH) on SK-Hep1 hepatocellular carcinoma (HCC) cells for lethal thermal effects with a self-made AMF system; an adjustable AMF frequency generated a variable intensity of magnetic field and induced MNP relaxation. The extracellular and intracellular MFH treatments on a SK-Hep1 cell line were implemented in vitro; the result indicates that the lethal effects were efficient and caused a significantly decreased cell viability of SK-Hep1 cells. As the PSS-MNP concentration decreased, especially in intracellular MFH treatments, the MFH effects on cells, however, largely decreased through heat spreading to the culture medium. On controlling and decreasing the volume of culture medium, the problem of heat spreading was solved. It can be consequently expected that PSS-MNPs would be a prospective agent for intracellular cancer magnetotherapy.
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spelling pubmed-78644582021-02-12 Extracellular and intracellular intermittent magnetic-fluid hyperthermia treatment of SK-Hep1 hepatocellular carcinoma cells based on magnetic nanoparticles coated with polystyrene sulfonic acid Chen, Bo-Wei Chiu, Guo-Wei He, Yun-Chi Huang, Chih-Yu Huang, Hao-Ting Sung, Shian-Ying Hsieh, Chia-Ling Chang, Wei-Chieh Hsu, Ming-Shinn Wei, Zung-Hang Yao, Da-Jeng PLoS One Research Article The use of magnetic nanoparticles (MNPs) magnetized on applying an alternating magnetic field (AMF) to stimulate the thermal characteristics and to induce tumor apoptosis is a currently active area of research in cancer treatment. In previous work, we developed biocompatible and superparamagnetic polystyrene-sulfonic-acid-coated magnetic nanoparticles (PSS-MNPs) as applications for magnetically labeled cell trapping, but without assessment of treatment effects on tumor diseases. In the present work, we examined PSS-MNP-induced magnetic fluid hyperthermia (MFH) on SK-Hep1 hepatocellular carcinoma (HCC) cells for lethal thermal effects with a self-made AMF system; an adjustable AMF frequency generated a variable intensity of magnetic field and induced MNP relaxation. The extracellular and intracellular MFH treatments on a SK-Hep1 cell line were implemented in vitro; the result indicates that the lethal effects were efficient and caused a significantly decreased cell viability of SK-Hep1 cells. As the PSS-MNP concentration decreased, especially in intracellular MFH treatments, the MFH effects on cells, however, largely decreased through heat spreading to the culture medium. On controlling and decreasing the volume of culture medium, the problem of heat spreading was solved. It can be consequently expected that PSS-MNPs would be a prospective agent for intracellular cancer magnetotherapy. Public Library of Science 2021-02-05 /pmc/articles/PMC7864458/ /pubmed/33544751 http://dx.doi.org/10.1371/journal.pone.0245286 Text en © 2021 Chen et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Chen, Bo-Wei
Chiu, Guo-Wei
He, Yun-Chi
Huang, Chih-Yu
Huang, Hao-Ting
Sung, Shian-Ying
Hsieh, Chia-Ling
Chang, Wei-Chieh
Hsu, Ming-Shinn
Wei, Zung-Hang
Yao, Da-Jeng
Extracellular and intracellular intermittent magnetic-fluid hyperthermia treatment of SK-Hep1 hepatocellular carcinoma cells based on magnetic nanoparticles coated with polystyrene sulfonic acid
title Extracellular and intracellular intermittent magnetic-fluid hyperthermia treatment of SK-Hep1 hepatocellular carcinoma cells based on magnetic nanoparticles coated with polystyrene sulfonic acid
title_full Extracellular and intracellular intermittent magnetic-fluid hyperthermia treatment of SK-Hep1 hepatocellular carcinoma cells based on magnetic nanoparticles coated with polystyrene sulfonic acid
title_fullStr Extracellular and intracellular intermittent magnetic-fluid hyperthermia treatment of SK-Hep1 hepatocellular carcinoma cells based on magnetic nanoparticles coated with polystyrene sulfonic acid
title_full_unstemmed Extracellular and intracellular intermittent magnetic-fluid hyperthermia treatment of SK-Hep1 hepatocellular carcinoma cells based on magnetic nanoparticles coated with polystyrene sulfonic acid
title_short Extracellular and intracellular intermittent magnetic-fluid hyperthermia treatment of SK-Hep1 hepatocellular carcinoma cells based on magnetic nanoparticles coated with polystyrene sulfonic acid
title_sort extracellular and intracellular intermittent magnetic-fluid hyperthermia treatment of sk-hep1 hepatocellular carcinoma cells based on magnetic nanoparticles coated with polystyrene sulfonic acid
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7864458/
https://www.ncbi.nlm.nih.gov/pubmed/33544751
http://dx.doi.org/10.1371/journal.pone.0245286
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