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Development of a robust protocol for the characterization of the pulmonary microbiota
The lack of methodological standardization diminishes the validity of results obtained and the conclusions drawn when studying the lung microbiota. We report the validation of a complete 16S rRNA gene amplicon sequencing workflow, from patient recruitment to bioinformatics, tailored to the constrain...
Autores principales: | Dumont-Leblond, Nathan, Veillette, Marc, Racine, Christine, Joubert, Philippe, Duchaine, Caroline |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7864980/ https://www.ncbi.nlm.nih.gov/pubmed/33547364 http://dx.doi.org/10.1038/s42003-021-01690-5 |
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