Structure and binding properties of Pangolin-CoV spike glycoprotein inform the evolution of SARS-CoV-2

Coronaviruses of bats and pangolins have been implicated in the origin and evolution of the pandemic SARS-CoV-2. We show that spikes from Guangdong Pangolin-CoVs, closely related to SARS-CoV-2, bind strongly to human and pangolin ACE2 receptors. We also report the cryo-EM structure of a Pangolin-CoV...

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Autores principales: Wrobel, Antoni G., Benton, Donald J., Xu, Pengqi, Calder, Lesley J., Borg, Annabel, Roustan, Chloë, Martin, Stephen R., Rosenthal, Peter B., Skehel, John J., Gamblin, Steven J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2021
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7864994/
https://www.ncbi.nlm.nih.gov/pubmed/33547281
http://dx.doi.org/10.1038/s41467-021-21006-9
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author Wrobel, Antoni G.
Benton, Donald J.
Xu, Pengqi
Calder, Lesley J.
Borg, Annabel
Roustan, Chloë
Martin, Stephen R.
Rosenthal, Peter B.
Skehel, John J.
Gamblin, Steven J.
author_facet Wrobel, Antoni G.
Benton, Donald J.
Xu, Pengqi
Calder, Lesley J.
Borg, Annabel
Roustan, Chloë
Martin, Stephen R.
Rosenthal, Peter B.
Skehel, John J.
Gamblin, Steven J.
author_sort Wrobel, Antoni G.
collection PubMed
description Coronaviruses of bats and pangolins have been implicated in the origin and evolution of the pandemic SARS-CoV-2. We show that spikes from Guangdong Pangolin-CoVs, closely related to SARS-CoV-2, bind strongly to human and pangolin ACE2 receptors. We also report the cryo-EM structure of a Pangolin-CoV spike protein and show it adopts a fully-closed conformation and that, aside from the Receptor-Binding Domain, it resembles the spike of a bat coronavirus RaTG13 more than that of SARS-CoV-2.
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spelling pubmed-78649942021-02-16 Structure and binding properties of Pangolin-CoV spike glycoprotein inform the evolution of SARS-CoV-2 Wrobel, Antoni G. Benton, Donald J. Xu, Pengqi Calder, Lesley J. Borg, Annabel Roustan, Chloë Martin, Stephen R. Rosenthal, Peter B. Skehel, John J. Gamblin, Steven J. Nat Commun Article Coronaviruses of bats and pangolins have been implicated in the origin and evolution of the pandemic SARS-CoV-2. We show that spikes from Guangdong Pangolin-CoVs, closely related to SARS-CoV-2, bind strongly to human and pangolin ACE2 receptors. We also report the cryo-EM structure of a Pangolin-CoV spike protein and show it adopts a fully-closed conformation and that, aside from the Receptor-Binding Domain, it resembles the spike of a bat coronavirus RaTG13 more than that of SARS-CoV-2. Nature Publishing Group UK 2021-02-05 /pmc/articles/PMC7864994/ /pubmed/33547281 http://dx.doi.org/10.1038/s41467-021-21006-9 Text en © The Author(s) 2021 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Wrobel, Antoni G.
Benton, Donald J.
Xu, Pengqi
Calder, Lesley J.
Borg, Annabel
Roustan, Chloë
Martin, Stephen R.
Rosenthal, Peter B.
Skehel, John J.
Gamblin, Steven J.
Structure and binding properties of Pangolin-CoV spike glycoprotein inform the evolution of SARS-CoV-2
title Structure and binding properties of Pangolin-CoV spike glycoprotein inform the evolution of SARS-CoV-2
title_full Structure and binding properties of Pangolin-CoV spike glycoprotein inform the evolution of SARS-CoV-2
title_fullStr Structure and binding properties of Pangolin-CoV spike glycoprotein inform the evolution of SARS-CoV-2
title_full_unstemmed Structure and binding properties of Pangolin-CoV spike glycoprotein inform the evolution of SARS-CoV-2
title_short Structure and binding properties of Pangolin-CoV spike glycoprotein inform the evolution of SARS-CoV-2
title_sort structure and binding properties of pangolin-cov spike glycoprotein inform the evolution of sars-cov-2
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7864994/
https://www.ncbi.nlm.nih.gov/pubmed/33547281
http://dx.doi.org/10.1038/s41467-021-21006-9
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