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Ph2 encodes the mismatch repair protein MSH7-3D that inhibits wheat homoeologous recombination
Meiotic recombination is a critical process for plant breeding, as it creates novel allele combinations that can be exploited for crop improvement. In wheat, a complex allohexaploid that has a diploid-like behaviour, meiotic recombination between homoeologous or alien chromosomes is suppressed throu...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7865012/ https://www.ncbi.nlm.nih.gov/pubmed/33547285 http://dx.doi.org/10.1038/s41467-021-21127-1 |
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author | Serra, Heïdi Svačina, Radim Baumann, Ute Whitford, Ryan Sutton, Tim Bartoš, Jan Sourdille, Pierre |
author_facet | Serra, Heïdi Svačina, Radim Baumann, Ute Whitford, Ryan Sutton, Tim Bartoš, Jan Sourdille, Pierre |
author_sort | Serra, Heïdi |
collection | PubMed |
description | Meiotic recombination is a critical process for plant breeding, as it creates novel allele combinations that can be exploited for crop improvement. In wheat, a complex allohexaploid that has a diploid-like behaviour, meiotic recombination between homoeologous or alien chromosomes is suppressed through the action of several loci. Here, we report positional cloning of Pairing homoeologous 2 (Ph2) and functional validation of the wheat DNA mismatch repair protein MSH7-3D as a key inhibitor of homoeologous recombination, thus solving a half-century-old question. Similar to ph2 mutant phenotype, we show that mutating MSH7-3D induces a substantial increase in homoeologous recombination (up to 5.5 fold) in wheat-wild relative hybrids, which is also associated with a reduction in homologous recombination. These data reveal a role for MSH7-3D in meiotic stabilisation of allopolyploidy and provides an opportunity to improve wheat’s genetic diversity through alien gene introgression, a major bottleneck facing crop improvement. |
format | Online Article Text |
id | pubmed-7865012 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-78650122021-02-16 Ph2 encodes the mismatch repair protein MSH7-3D that inhibits wheat homoeologous recombination Serra, Heïdi Svačina, Radim Baumann, Ute Whitford, Ryan Sutton, Tim Bartoš, Jan Sourdille, Pierre Nat Commun Article Meiotic recombination is a critical process for plant breeding, as it creates novel allele combinations that can be exploited for crop improvement. In wheat, a complex allohexaploid that has a diploid-like behaviour, meiotic recombination between homoeologous or alien chromosomes is suppressed through the action of several loci. Here, we report positional cloning of Pairing homoeologous 2 (Ph2) and functional validation of the wheat DNA mismatch repair protein MSH7-3D as a key inhibitor of homoeologous recombination, thus solving a half-century-old question. Similar to ph2 mutant phenotype, we show that mutating MSH7-3D induces a substantial increase in homoeologous recombination (up to 5.5 fold) in wheat-wild relative hybrids, which is also associated with a reduction in homologous recombination. These data reveal a role for MSH7-3D in meiotic stabilisation of allopolyploidy and provides an opportunity to improve wheat’s genetic diversity through alien gene introgression, a major bottleneck facing crop improvement. Nature Publishing Group UK 2021-02-05 /pmc/articles/PMC7865012/ /pubmed/33547285 http://dx.doi.org/10.1038/s41467-021-21127-1 Text en © The Author(s) 2021 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Serra, Heïdi Svačina, Radim Baumann, Ute Whitford, Ryan Sutton, Tim Bartoš, Jan Sourdille, Pierre Ph2 encodes the mismatch repair protein MSH7-3D that inhibits wheat homoeologous recombination |
title | Ph2 encodes the mismatch repair protein MSH7-3D that inhibits wheat homoeologous recombination |
title_full | Ph2 encodes the mismatch repair protein MSH7-3D that inhibits wheat homoeologous recombination |
title_fullStr | Ph2 encodes the mismatch repair protein MSH7-3D that inhibits wheat homoeologous recombination |
title_full_unstemmed | Ph2 encodes the mismatch repair protein MSH7-3D that inhibits wheat homoeologous recombination |
title_short | Ph2 encodes the mismatch repair protein MSH7-3D that inhibits wheat homoeologous recombination |
title_sort | ph2 encodes the mismatch repair protein msh7-3d that inhibits wheat homoeologous recombination |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7865012/ https://www.ncbi.nlm.nih.gov/pubmed/33547285 http://dx.doi.org/10.1038/s41467-021-21127-1 |
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